NKX3.1 Antibody - #AF0221

(2)  
Product: NKX3.1 Antibody
Catalog: AF0221
Description: Rabbit polyclonal antibody to NKX3.1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 38kDa; 26kD(Calculated).
Uniprot: Q99801
RRID: AB_2834156

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Horse(82%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
NKX3.1 Antibody detects endogenous levels of total NKX3.1.
RRID:
AB_2834156
Cite Format: Affinity Biosciences Cat# AF0221, RRID:AB_2834156.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BAPX 2; BAPX2; Homeobox protein NK-3 homolog A; Homeobox protein Nkx 3.1; Homeobox protein Nkx-3.1; Homeobox protein Nkx3.1; NK homeobox (Drosophila) family 3 A; NK homeobox family 3 A; NK homeobox, family 3, member A; NK3 homeobox 1; NK3 transcription factor homolog A; NK3 transcription factor related locus 1; NKX 3; Nkx 3.1; NKX 3A; NKX3 1; NKX3; Nkx3-1; NKX3.1; Nkx3.1, mouse, homolog of; NKX31_HUMAN; NKX3A;

Immunogens

Immunogen:
Uniprot:

Q99801(NKX31_HUMAN) >>Visit HPA database.

Gene(ID):
NKX3-1(4824)
Expression:
Q99801 NKX31_HUMAN:

Highly expressed in the prostate and, at a lower level, in the testis.

Description:
NKX3-1 Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Interacts with serum response factor (SRF).
Sequence:
MLRVPEPRPGEAKAEGAAPPTPSKPLTSFLIQDILRDGAQRQGGRTSSQRQRDPEPEPEPEPEGGRSRAGAQNDQLSTGPRAAPEEAETLAETEPERHLGSYLLDSENTSGALPRLPQTPKQPQKRSRAAFSHTQVIELERKFSHQKYLSAPERAHLAKNLKLTETQVKIWFQNRRYKTKRKQLSSELGDLEKHSSLPALKEEAFSRASLVSVYNSYPYYPYLYCVGSWSPAFW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Dog
91
Rabbit
91
Horse
82
Bovine
73
Sheep
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99801 As Substrate

Site PTM Type Enzyme
T21 Phosphorylation
S48 Phosphorylation P17252 (PRKCA)
T89 Phosphorylation P11309 (PIM1) , P19784 (CSNK2A2)
T93 Phosphorylation P19784 (CSNK2A2)
T134 Phosphorylation Q13315 (ATM)
K159 Ubiquitination
K162 Ubiquitination
T166 Phosphorylation Q13315 (ATM)
K182 Ubiquitination
S185 Phosphorylation P11309 (PIM1) , Q9Y463 (DYRK1B)
S186 Phosphorylation P11309 (PIM1)
S195 Phosphorylation P11309 (PIM1)
S196 Phosphorylation P11309 (PIM1)
Y222 Phosphorylation

Research Backgrounds

Function:

Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.

PTMs:

Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in the prostate and, at a lower level, in the testis.

Subunit Structure:

Interacts with serum response factor (SRF) (By similarity). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation.

Family&Domains:

Belongs to the NK-3 homeobox family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

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