GRP94 Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/3

GRP94 Antibody - AF0735 at 1/100 staining human brain tissue sections by IHC-P.

GRP94 Antibody - AF0735 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC.

GRP94 Antibody - Figure 5 Validation of potential protein targets.

Product:	GRP94 Antibody
Catalog:	AF0735
Description:	Rabbit polyclonal antibody to GRP94
Application:	WB IHC IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	92kDa; 92kD(Calculated).
Uniprot:	P14625
RRID:	AB_2834142

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

GRP94 Antibody detects endogenous levels of total GRP94.

RRID:

AB_2834142
Cite Format: Affinity Biosciences Cat# AF0735, RRID:AB_2834142.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

94 kDa glucose regulated protein; 94 kDa glucose-regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; ENPL_HUMAN; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; Heat shock protein, 90 kDa, beta, 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; Tumor rejection antigen-1 (gp96);

Immunogens

Immunogen:

A synthesized peptide derived from human GRP94, corresponding to a region within C-terminal amino acids.

Uniprot:

P14625(ENPL_HUMAN) >>Visit HPA database.

Gene(ID):

HSP90B1(7184)

Description:

GRP94 Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5.

Sequence:

P14625 ENPL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTAEKDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Sheep

Dog

Rabbit

Horse

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P14625_ENPL_HUMAN

Function:

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).

Subcellular Location:

Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Family&Domains:

Belongs to the heat shock protein 90 family.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

References

1). C1QA and COMP: plasma-based biomarkers for early diagnosis of pancreatic neuroendocrine tumors. Scientific reports, 2023 (PubMed: 38030709) [IF=3.8]

Application: WB Species: Human Sample:

Figure 5 Validation of potential protein targets. Western blot profiling and densitometric analysis of final selected proteins (A). Densitometric analysis of (B) C1QA (p = 0.001, 0.03, 0.0013); (C) COMP protein (p = 0.011, 0.019), (D) HSP90B1, (E) ITGA2B/CD41, (F) FN1, (G, H) Sandwich ELISA for C1QA binding analysis (I) ROC curve analysis for plasma C1QA levels in Grade I PanNET vs controls (J, K) Sandwich ELISA for COMP analysis (I) ROC curve analysis for plasma COMP levels in Grade I PanNET vs controls.

2). Proteomics analysis of serum and urine identifies VCP and CTSA as potential biomarkers associated with multiple myeloma. Clinica chimica acta; international journal of clinical chemistry, 2024 (PubMed: 38081446) [IF=3.2]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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GRP94 Antibody - #AF0735