GAD1/2 Antibody - #AF0711
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0711, RRID:AB_2834138.
Fold/Unfold
67 kDa glutamic acid decarboxylase; CPSQ1; DCE1; DCE1_HUMAN; EC 4.1.1.15; FLJ45882; GAD 67; GAD; GAD-67; GAD1; Glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 1; Glutamate decarboxylase 1 brain 67kD; Glutamate decarboxylase 1 brain 67kDa; Glutamate decarboxylase 67 kDa isoform; Glutamate decarboxylase, brain, 67-KD; OTTHUMP00000041055; SCP; 65 kDa glutamic acid decarboxylase; DCE 2; DCE2; DCE2_HUMAN; GAD 2; GAD 65; GAD-2; GAD-65; Gad2; Glutamate decarboxylase 2 (pancreas); Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa); Glutamate decarboxylase 2; Glutamate Decarboxylase 65; Glutamate decarboxylase 65 kDa isoform; Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65; MGC161605; MGC161607;
Immunogens
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
- Q99259 DCE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASSTPSSSATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDARFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIVGWSSKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEQSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADFEAKILEAKQKGYVPFYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLNGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPQRREKLHKVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL
- Q05329 DCE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASPGSGFWSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGNKLCALLYGDAEKPAESGGSQPPRAAARKAACACDQKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99259/Q05329 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K60 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| T91 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y265 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S578 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| Y169 | Phosphorylation | Uniprot | |
| T296 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| T515 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| Y547 | Phosphorylation | Uniprot |
Research Backgrounds
Catalyzes the production of GABA.
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.
Homodimer.
Belongs to the group II decarboxylase family.
Catalyzes the production of GABA.
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.
Cytoplasm>Cytosol. Cytoplasmic vesicle. Cell junction>Synapse>Presynaptic cell membrane>Lipid-anchor. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
Homodimer.
Belongs to the group II decarboxylase family.
Research Fields
· Human Diseases > Endocrine and metabolic diseases > Type I diabetes mellitus.
· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.
· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.
· Metabolism > Metabolism of other amino acids > Taurine and hypotaurine metabolism.
· Metabolism > Carbohydrate metabolism > Butanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Nervous system > GABAergic synapse.
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