MMP3 Antibody | Affinity Biosciences

Product:	MMP3 Antibody
Catalog:	AF0217
Description:	Rabbit polyclonal antibody to MMP3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Sheep, Rabbit, Dog
Mol.Wt.:	53kDa; 54kD(Calculated).
Uniprot:	P08254
RRID:	AB_2833347

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(100%), Sheep(87%), Rabbit(92%), Dog(93%)

Clonality:

Polyclonal

Specificity:

MMP3 Antibody detects endogenous levels of total MMP3.

RRID:

AB_2833347
Cite Format: Affinity Biosciences Cat# AF0217, RRID:AB_2833347.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP 3; MMP-3; MMP3; MMP3_HUMAN; Proteoglycanase; SL-1; SL1; STMY; STMY1; STR1; Stromelysin 1; Stromelysin-1; Transin 1; Transin-1;

Immunogens

Immunogen:

Uniprot:

P08254(MMP3_HUMAN) >>Visit HPA database.

Gene(ID):

MMP3(4314)

Description:

MMP3 Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Belongs to the peptidase M10A family. Note: This description may include information from UniProtKB.

Sequence:

P08254 MMP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKSLPILLLLCVAVCSAYPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

Rabbit

Sheep

Xenopus

Pig

Bovine

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08254 As Substrate

P08254

Site	PTM Type	Source
Y18	Phosphorylation	Uniprot
Y116	Phosphorylation	Uniprot
Y121	Phosphorylation	Uniprot
K127	Ubiquitination	Uniprot

Research Backgrounds

P08254_MMP3_HUMAN

Function:

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

References

1). Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation. PHYTOMEDICINE, 2021 (PubMed: 34371251) [IF=7.9]

Application: WB Species: Rat Sample: chondrocytes

Fig. 2. Effects of avicularin on ECM degradation. (A-D) The mRNA levels of ACAN, Collagen 2, MMP3 and MMP13 in rat and human chondrocytes were detected by qPCR. The dates were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the IL-1β-induced group, n = 3. (E-F) The expression of MMP3, MMP13, ADAMTS5, SOX9, ACAN and Collagen II was detected by western blots.

Application: IHC Species: Rat Sample: chondrocytes

Fig. 7. Avicularin attenuates ECM degradation in ACLT rats. (A) ACAN, (B) Collagen II, (C) MMP3, (D) MMP13 and (E) TRAF6 immunohistochemistry of knee joint medial compartment cartilage. (F-J) Quantitative analysis of ACAN, Collagen II, MMP3, MMP13 and TRAF6. The values were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the ACLT group, n = 6. (K) The working model of how avicularin protects against OA development.

2). Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota. Phytomedicine, 2022 (PubMed: 36162242) [IF=7.9]

3). Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stress. PHYTOMEDICINE, 2022 (PubMed: 35905566) [IF=7.9]

4). Vitamin K2 ameliorates osteoarthritis by suppressing ferroptosis and extracellular matrix degradation through activation GPX4's dual functions. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38759289) [IF=7.5]

Application: WB Species: Rat Sample:

Fig. 3. VK2 rescues rat primary chondrocytes from TBHP-Induced cell death and ECM degradation. (A) The working concentration of VK in vitro was screened by CCK8; (B) Flow cytometry detection of apoptosis; (C) Flow cytometry statistics for detection of apoptosis; (D) Representative pictures of chondrocytes of toluidine blue staining (Scale bar=200μm); (E-J) WB results for ACAN, COL2A1, SOX9, MMP3, MMP13 and Tubulin.

5). Biochanin A protects against iron overload associated knee osteoarthritis via regulating iron levels and NRF2/System xc-/GPX4 axis. Biomedicine & Pharmacotherapy, 2023 (PubMed: 36379122) [IF=7.5]

6). Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34311530) [IF=7.5]

7). White adipose tissue, a novel antirheumatic target: Clues from its secretory capability and adipectomy-based therapy. British journal of pharmacology, 2024 (PubMed: 38644540) [IF=7.3]

8). A non-retinol retinoic acid receptor-γ (RAR-γ/NR1B3) selective agonist, tectorigenin, can effectively inhibit the ultraviolet A-induced skin damage. British journal of pharmacology, 2022 (PubMed: 35731978) [IF=7.3]

9). Quercetin-3-O-β-D-glucuronide attenuates osteoarthritis by inhibiting cartilage extracellular matrix degradation and inflammation. Journal of orthopaedic translation, 2024 (PubMed: 38601200) [IF=6.6]

10). TGF-β induced PAR-1 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone. International Journal of Cancer, 2017 (PubMed: 28670703) [IF=6.4]

Application: IHC Species: human Sample: GCTB

Supplementary Figure 1 | The expression of MMP3 in GCT of bone. The expression level of MMP3 showed no significant correlation with TGF-β and PAR-1 expression.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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MMP3 Antibody - #AF0217