MMP3 Antibody | Affinity Biosciences

Product:	MMP3 Antibody
Catalog:	AF0217
Description:	Rabbit polyclonal antibody to MMP3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Sheep, Rabbit, Dog
Mol.Wt.:	53kDa; 54kD(Calculated).
Uniprot:	P08254
RRID:	AB_2833347

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(100%), Sheep(87%), Rabbit(92%), Dog(93%)

Clonality:

Polyclonal

Specificity:

MMP3 Antibody detects endogenous levels of total MMP3.

RRID:

AB_2833347
Cite Format: Affinity Biosciences Cat# AF0217, RRID:AB_2833347.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CHDS6; Matrix metalloproteinase 3; Matrix metalloproteinase-3; MGC126102; MGC126103; MGC126104; MMP 3; MMP-3; MMP3; MMP3_HUMAN; Proteoglycanase; SL-1; SL1; STMY; STMY1; STR1; Stromelysin 1; Stromelysin-1; Transin 1; Transin-1;

Immunogens

Immunogen:

Uniprot:

P08254(MMP3_HUMAN) >>Visit HPA database.

Gene(ID):

MMP3(4314)

Description:

MMP3 Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. Belongs to the peptidase M10A family. Note: This description may include information from UniProtKB.

Sequence:

P08254 MMP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKSLPILLLLCVAVCSAYPLDGAARGEDTSMNLVQKYLENYYDLKKDVKQFVRRKDSGPVVKKIREMQKFLGLEVTGKLDSDTLEVMRKPRCGVPDVGHFRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

Rabbit

Sheep

Xenopus

Pig

Bovine

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08254 As Substrate

P08254

Site	PTM Type	Source
Y18	Phosphorylation	Uniprot
Y116	Phosphorylation	Uniprot
Y121	Phosphorylation	Uniprot
K127	Ubiquitination	Uniprot

Research Backgrounds

P08254_MMP3_HUMAN

Function:

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

Research Fields

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

References

1). Avicularin suppresses cartilage extracellular matrix degradation and inflammation via TRAF6/MAPK activation. PHYTOMEDICINE, 2021 (PubMed: 34371251) [IF=7.9]

Application: WB Species: Rat Sample: chondrocytes

Fig. 2. Effects of avicularin on ECM degradation. (A-D) The mRNA levels of ACAN, Collagen 2, MMP3 and MMP13 in rat and human chondrocytes were detected by qPCR. The dates were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the IL-1β-induced group, n = 3. (E-F) The expression of MMP3, MMP13, ADAMTS5, SOX9, ACAN and Collagen II was detected by western blots.

Application: IHC Species: Rat Sample: chondrocytes

Fig. 7. Avicularin attenuates ECM degradation in ACLT rats. (A) ACAN, (B) Collagen II, (C) MMP3, (D) MMP13 and (E) TRAF6 immunohistochemistry of knee joint medial compartment cartilage. (F-J) Quantitative analysis of ACAN, Collagen II, MMP3, MMP13 and TRAF6. The values were shown as the means ± S.D. ** p < 0.01 and *** p < 0.001 compared with the control group; # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the ACLT group, n = 6. (K) The working model of how avicularin protects against OA development.

2). Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota. Phytomedicine, 2022 (PubMed: 36162242) [IF=7.9]

3). Naringenin protects against iron overload-induced osteoarthritis by suppressing oxidative stress. PHYTOMEDICINE, 2022 (PubMed: 35905566) [IF=7.9]

4). Biochanin A protects against iron overload associated knee osteoarthritis via regulating iron levels and NRF2/System xc-/GPX4 axis. Biomedicine & Pharmacotherapy, 2023 (PubMed: 36379122) [IF=7.5]

5). Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. Biomedicine & Pharmacotherapy, 2021 (PubMed: 34311530) [IF=7.5]

6). TGF-β induced PAR-1 expression promotes tumor progression and osteoclast differentiation in giant cell tumor of bone. International Journal of Cancer, 2017 (PubMed: 28670703) [IF=6.4]

Application: IHC Species: human Sample: GCTB

Supplementary Figure 1 | The expression of MMP3 in GCT of bone. The expression level of MMP3 showed no significant correlation with TGF-β and PAR-1 expression.

7). PDLIM2 protects articular chondrocytes from lipopolysaccharide-induced apoptosis, degeneration and inflammatory injury through down-regulation of nuclear factor (NF)-κB signaling. International Immunopharmacology, 2020 (PubMed: 32805696) [IF=5.6]

Application: WB Species: Mouse Sample: chondrocytes

Fig. 2. Up-regulation of PDLIM2 attenuates LPS-induced injury in chondrocytes. Chondrocytes were transfected with empty vector (EV) or PDLIM2 expression vector for 48 h and then stimulated with 10 μg/ml of LPS for 6 h. (A) The relative mRNA expression of PDLIM2 was determined by RT-qPCR. (B, C) Protein expression of PDLIM2 was examined by Western blotting. (D) The effect of PDLIM2 overexpression on cell viability was detected by MTT assay. The effect of PDLIM2 overexpression on cell apoptosis was assessed by (E, F) TUNENL and (G, H) Annexin V-FITC/PI apoptosis assays. (I) The effect of PDLIM2 overexpression on MMP-3, MMP-13, COL2A1 and ACAN was evaluated by Western blotting. Quantitative analysis of protein expression of (J) MMP-3, (K) MMP-13, (L) COL2A1 and (M) ACAN. N = 3, **p < 0.01 and ***p < 0.001.

8). Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation. World Journal of Stem Cells, 2022 (PubMed: 36483847) [IF=4.1]

9). Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study. Experimental gerontology, 2024 (PubMed: 38570055) [IF=3.9]

Application: WB Species: Rat Sample:

Fig. 3. Emo mitigates cartilage damage in the rats of the OP-OA group (red arrows: degree of wear and tear of articular cartilage). (A) H&E and SO&FG staining. (B) ELISA assay results. (C, D) Immunoblot analysis of SOX9, COL2A1, MMP3, and MMP13. (E) Real-time PCR findings of SOX9, COL2A1, MMP3, and MMP13. Data are presented as mean ± standard deviation. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

10). Deciphering the pharmacological mechanisms of Fraxini Cortex for ulcerative colitis treatment based on network pharmacology and in vivo studies. BMC Complementary Medicine and Therapies, 2023 (PubMed: 37161415) [IF=3.9]

Application: WB Species: Mouse Sample:

Fig. 11 FC regulated the expression of key target genes. The protein expression levels of (A-B) IL-1β, COX2, (C-D) IL-17, RORγt, and (E-F) MMP1, MMP3 and MMP9 were measured by western blot. Data are shown as the mean ± SD, #P

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MMP3 Antibody - #AF0217