Claudin 7 Antibody - #AF0521

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Product: Claudin 7 Antibody
Catalog: AF0521
Description: Rabbit polyclonal antibody to Claudin 7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 22kDa; 22kD(Calculated).
Uniprot: O95471
RRID: AB_2834127

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Rabbit(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
Claudin 7 Antibody detects endogenous levels of total Claudin 7.
RRID:
AB_2834127
Cite Format: Affinity Biosciences Cat# AF0521, RRID:AB_2834127.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CEPTR L2; CEPTRL 2; CEPTRL2; Claudin 1; Claudin 9; Claudin-7; Claudin1; Claudin7; Claudin9; CLD7_HUMAN; CLDN 7; CLDN-7; CLDN7; Clostridium perfringens enterotoxin receptor like 2; CPETR L2; CPETRL 2; CPETRL2; Hs.84359;

Immunogens

Immunogen:
Uniprot:

O95471(CLD7_HUMAN) >>Visit HPA database.

Gene(ID):
CLDN7(1366)
Expression:
O95471 CLD7_HUMAN:

Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.

Description:
Claudins, such as CLDN7, are involved in the formation of tight junctions between epithelial cells. Tight junctions restrict lateral diffusion of lipids and membrane proteins, and thereby physically define the border between the apical and basolateral compartments of epithelial cells (Zheng et al., 2003).[supplied by OMIM]
Sequence:
MANSGLQLLGFSMALLGWVGLVACTAIPQWQMSSYAGDNIITAQAMYKGLWMDCVTQSTGMMSCKMYDSVLALSAALQATRALMVVSLVLGFLAMFVATMGMKCTRCGGDDKVKKARIAMGGGIIFIVAGLAALVACSWYGHQIVTDFYNPLIPTNIKYEFGPAIFIGWAGSALVILGGALLSCSCPGNESKAGYRVPRSYPKSNSSKEYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Horse
83
Bovine
83
Sheep
83
Dog
83
Rabbit
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95471 As Substrate

Site PTM Type Enzyme
Y67 Phosphorylation
T99 Phosphorylation
Y195 Phosphorylation
S200 Phosphorylation
K203 Ubiquitination
S204 Phosphorylation P17252 (PRKCA)
S206 Phosphorylation Q96J92 (WNK4)
S207 Phosphorylation
K208 Ubiquitination
Y210 Phosphorylation

Research Backgrounds

Function:

Plays a major role in tight junction-specific obliteration of the intercellular space.

PTMs:

Phosphorylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Basolateral cell membrane. Cell junction>Tight junction.
Note: Co-localizes with EPCAM at the basolateral cell membrane and tight junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.

Subunit Structure:

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity). The phosphorylated form interacts with EPCAM. Does not interact with CD81.

Family&Domains:

Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). Commensal cow Roseburia reduces gut-dysbiosis-induced mastitis through inhibiting bacterial translocation by producing butyrate in mice. Cell Reports, 2022 (PubMed: 36417859) [IF=8.8]

Application: WB    Species: Mouse    Sample:

Figure 5Commensal Roseburia ameliorates gut-dysbiosis-induced mastitis in mice

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