Phospho-5 Lipoxygenase (Ser523) Antibody - #AF0064

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Product: Phospho-5 Lipoxygenase (Ser523) Antibody
Catalog: AF0064
Description: Rabbit polyclonal antibody to Phospho-5 Lipoxygenase (Ser523)
Application: WB IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 77kDa; 78kD(Calculated).
Uniprot: P09917
RRID: AB_2834107

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(86%), Bovine(100%), Sheep(86%), Rabbit(100%), Dog(86%)
Clonality:
Polyclonal
Specificity:
Phospho-5 Lipoxygenase (Ser523) Antibody detects endogenous levels of 5 Lipoxygenase only when phosphorylated at Sersine 523.
RRID:
AB_2834107
Cite Format: Affinity Biosciences Cat# AF0064, RRID:AB_2834107.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5 LO; 5 LOX; 5-lipoxygenase; 5-LO; 5-LOX; 5LOX; 5LPG; ALOX 5; Alox5; Arachidonate 5 lipoxygenase; Arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; LOX5_HUMAN; MGC163204;

Immunogens

Immunogen:
Uniprot:

P09917(LOX5_HUMAN) >>Visit HPA database.

Gene(ID):
ALOX5(240)
Description:
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
Sequence:
MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
100
Pig
86
Sheep
86
Dog
86
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09917 As Substrate

Site PTM Type Enzyme
Y21 Phosphorylation
Y23 Phosphorylation
Y43 Phosphorylation
Y54 Phosphorylation
Y75 Phosphorylation
T87 Phosphorylation
Y95 Phosphorylation
Y101 Phosphorylation
T105 Phosphorylation
Y182 Phosphorylation
S183 Phosphorylation
T250 Phosphorylation
T259 Phosphorylation
T260 Phosphorylation
S266 Phosphorylation
S272 Phosphorylation Q9UQM7 (CAMK2A) , P17612 (PRKACA) , P49137 (MAPKAPK2)
T445 Phosphorylation
Y446 Phosphorylation
S448 Phosphorylation
T486 Phosphorylation
S523 Phosphorylation
S524 Phosphorylation P17612 (PRKACA)
K528 Acetylation
S532 Phosphorylation
S664 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
S671 Phosphorylation

Research Backgrounds

Function:

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

PTMs:

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

Subcellular Location:

Cytoplasm. Nucleus matrix. Nucleus membrane>Peripheral membrane protein.
Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity.

Family&Domains:

Belongs to the lipoxygenase family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

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