Phospho-5 Lipoxygenase (Ser523) Antibody

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Product:	Phospho-5 Lipoxygenase (Ser523) Antibody
Catalog:	AF0064
Description:	Rabbit polyclonal antibody to Phospho-5 Lipoxygenase (Ser523)
Application:	WB IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	77kDa; 78kD(Calculated).
Uniprot:	P09917
RRID:	AB_2834107

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(86%), Bovine(100%), Sheep(86%), Rabbit(100%), Dog(86%)

Clonality:

Polyclonal

Specificity:

Phospho-5 Lipoxygenase (Ser523) Antibody detects endogenous levels of 5 Lipoxygenase only when phosphorylated at Sersine 523.

RRID:

AB_2834107
Cite Format: Affinity Biosciences Cat# AF0064, RRID:AB_2834107.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5 LO; 5 LOX; 5-lipoxygenase; 5-LO; 5-LOX; 5LOX; 5LPG; ALOX 5; Alox5; Arachidonate 5 lipoxygenase; Arachidonate 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; LOX5_HUMAN; MGC163204;

Immunogens

Immunogen:

Uniprot:

P09917(LOX5_HUMAN) >>Visit HPA database.

Gene(ID):

ALOX5(240)

Description:

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Sequence:

P09917 LOX5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWLLAKIWVRSSDFHVHQTITHLLRTHLVSEVFGIAMYRQLPAVHPIFKLLVAHVRFTIAINTKAREQLICECGLFDKANATGGGGHVQMVQRAMKDLTYASLCFPEAIKARGMESKEDIPYYFYRDDGLLVWEAIRTFTAEVVDIYYEGDQVVEEDPELQDFVNDVYVYGMRGRKSSGFPKSVKSREQLSEYLTVVIFTASAQHAAVNFGQYDWCSWIPNAPPTMRAPPPTAKGVVTIEQIVDTLPDRGRSCWHLGAVWALSQFQENELFLGMYPEEHFIEKPVKEAMARFRKNLEAIVSVIAERNKKKQLPYYYLSPDRIPNSVAI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Bovine

100

Pig

Sheep

Dog

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09917 As Substrate

P09917

Site	PTM Type	Enzyme	Source
Y21	Phosphorylation		Uniprot
Y23	Phosphorylation		Uniprot
Y43	Phosphorylation		Uniprot
Y54	Phosphorylation		Uniprot
Y75	Phosphorylation		Uniprot
T87	Phosphorylation		Uniprot
Y95	Phosphorylation		Uniprot
Y101	Phosphorylation		Uniprot
T105	Phosphorylation		Uniprot
Y182	Phosphorylation		Uniprot
S183	Phosphorylation		Uniprot
T250	Phosphorylation		Uniprot
T259	Phosphorylation		Uniprot
T260	Phosphorylation		Uniprot
S266	Phosphorylation		Uniprot
S272	Phosphorylation	Q9UQM7 (CAMK2A) , P17612 (PRKACA) , P49137 (MAPKAPK2)	Uniprot
T445	Phosphorylation		Uniprot
Y446	Phosphorylation		Uniprot
S448	Phosphorylation		Uniprot
T486	Phosphorylation		Uniprot
S523	Phosphorylation		Uniprot
S524	Phosphorylation	P17612 (PRKACA)	Uniprot
K528	Acetylation		Uniprot
S532	Phosphorylation		Uniprot
S664	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
S671	Phosphorylation		Uniprot

Research Backgrounds

P09917_LOX5_HUMAN

Function:

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

PTMs:

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

Subcellular Location:

Cytoplasm. Nucleus matrix. Nucleus membrane>Peripheral membrane protein.
Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-272. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association.

Subunit Structure:

Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity.

Family&Domains:

Belongs to the lipoxygenase family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-5 Lipoxygenase (Ser523) Antibody - #AF0064