Phospho-EPS15 (Tyr849) Antibody | Affinity Biosciences

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AF0058 at 1/100 staining Mouse muscle tissue by IHC-P.

Product:	Phospho-EPS15 (Tyr849) Antibody
Catalog:	AF0058
Description:	Rabbit polyclonal antibody to Phospho-EPS15 (Tyr849)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	98kDa; 99kD(Calculated).
Uniprot:	P42566
RRID:	AB_2834101

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Phospho-EPS15 (Tyr849) Antibody detects endogenous levels of EPS15 only when phosphorylated at Tyrosine 849.

RRID:

AB_2834101
Cite Format: Affinity Biosciences Cat# AF0058, RRID:AB_2834101.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AF 1P; AF 1p protein; AF1P; ALL1 fused gene from chromosome 1; Epidermal growth factor receptor pathway substrate 15; Epidermal growth factor receptor substrate 15; EPS 15; MLLT 5; MLLT5; Protein Eps 15; Protein Eps15;

Immunogens

Immunogen:

A synthesized peptide derived from human EPS15 around the phosphorylation site of Tyr849.

Uniprot:

P42566(EPS15_HUMAN) >>Visit HPA database.

Gene(ID):

EPS15(2060)

Expression:

P42566 EPS15_HUMAN:

Ubiquitously expressed.

Description:

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Sequence:

P42566 EPS15_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNKQEFFVALRLVACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKLPVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNIIGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQHLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPARSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA

PTMs - P42566 As Substrate

P42566

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
S8	Phosphorylation		Uniprot
T106	Phosphorylation		Uniprot
S107	Phosphorylation		Uniprot
S108	Phosphorylation		Uniprot
S140	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot
K227	Methylation		Uniprot
K227	Ubiquitination		Uniprot
K234	Methylation		Uniprot
K234	Ubiquitination		Uniprot
S244	Phosphorylation		Uniprot
T304	Phosphorylation		Uniprot
S315	Phosphorylation		Uniprot
K318	Ubiquitination		Uniprot
S323	Phosphorylation		Uniprot
S324	Phosphorylation		Uniprot
K333	Ubiquitination		Uniprot
K357	Acetylation		Uniprot
K359	Acetylation		Uniprot
K386	Ubiquitination		Uniprot
K391	Ubiquitination		Uniprot
K413	Ubiquitination		Uniprot
S435	Phosphorylation		Uniprot
Y443	Phosphorylation		Uniprot
K449	Ubiquitination		Uniprot
S455	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot
S470	Phosphorylation		Uniprot
K472	Ubiquitination		Uniprot
S485	Phosphorylation		Uniprot
K495	Ubiquitination		Uniprot
S551	Phosphorylation		Uniprot
S558	Phosphorylation		Uniprot
S562	Phosphorylation		Uniprot
S563	Phosphorylation		Uniprot
S587	Phosphorylation		Uniprot
S595	Phosphorylation		Uniprot
S605	Phosphorylation		Uniprot
S606	Phosphorylation		Uniprot
K632	Ubiquitination		Uniprot
K638	Ubiquitination		Uniprot
K648	Ubiquitination		Uniprot
S655	Phosphorylation		Uniprot
T663	Phosphorylation		Uniprot
S680	Phosphorylation		Uniprot
S681	Phosphorylation		Uniprot
S719	Phosphorylation		Uniprot
S741	Phosphorylation		Uniprot
S746	Phosphorylation		Uniprot
K754	Ubiquitination		Uniprot
K763	Ubiquitination		Uniprot
K774	Ubiquitination		Uniprot
T777	Phosphorylation		Uniprot
T779	Phosphorylation		Uniprot
K788	Acetylation		Uniprot
K788	Ubiquitination		Uniprot
S790	Phosphorylation		Uniprot
K793	Ubiquitination		Uniprot
S796	Phosphorylation	Q16539 (MAPK14)	Uniprot
K801	Ubiquitination		Uniprot
S814	Phosphorylation		Uniprot
K816	Ubiquitination		Uniprot
K818	Ubiquitination		Uniprot
K836	Ubiquitination		Uniprot
Y849	Phosphorylation	P00533 (EGFR)	Uniprot
Y850	Phosphorylation		Uniprot
S851	Phosphorylation		Uniprot
K860	Ubiquitination		Uniprot
K890	Ubiquitination		Uniprot

Research Backgrounds

P42566_EPS15_HUMAN

Function:

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

PTMs:

Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.

Ubiquitinated.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Clathrin-coated pit.
Note: Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes.

Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Colocalizes with HGS on bilayered clathrin coats on endosomes.

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with SGIP1. Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2. Interacts (via UIM repeats) with CORO7 (when ubiquitinated at 'Lys-472'). Interacts (via UIM domains) with UBQLN1 (via ubiquitin-like domain) and can interact with both the ubiquitinated and the non-ubiquitinated forms of UBQLN1. Interacts with UBQLN2 (By similarity).

(Microbial infection) Interacts with vaccinia virus protein A36.

Family&Domains:

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

The UIM (ubiquitin-interacting motif) repeats specifically bind 'Lys-33'-linked ubiquitin.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

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Phospho-EPS15 (Tyr849) Antibody - #AF0058