Phospho-FANCG (Ser383) Antibody - #AF0055

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Product: Phospho-FANCG (Ser383) Antibody
Catalog: AF0055
Description: Rabbit polyclonal antibody to Phospho-FANCG (Ser383)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 69kDa; 69kD(Calculated).
Uniprot: O15287
RRID: AB_2834098

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Phospho-FANCG (Ser383) Antibody detects endogenous levels of FANCG only when phosphorylated at Sersine 383.
RRID:
AB_2834098
Cite Format: Affinity Biosciences Cat# AF0055, RRID:AB_2834098.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA repair protein XRCC9; FAG; FANCG; FANCG_HUMAN; Fanconi anaemia complementation group G; Fanconi anemia group G protein; Protein FACG; X ray repair, complementing defective, in Chinese hamster cells 9; X-ray repair, complementing defective, in Chinese hamster, 9; XRCC9;

Immunogens

Immunogen:

A synthesized peptide derived from human FANCG around the phosphorylation site of Ser383.

Uniprot:

O15287(FANCG_HUMAN) >>Visit HPA database.

Gene(ID):
FANCG(2189)
Expression:
O15287 FANCG_HUMAN:

Highly expressed in testis and thymus. Found in lymphoblasts.

Description:
FANCG DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene. Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. Note: This description may include information from UniProtKB.
Sequence:
MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGLTLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLELTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLETQEQQGPRLEQGLRELWDSVLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHILASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPPPSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEELLSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSATHLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEEKEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWVASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKRLDRRDEATALWWRLEAQTKGSHEDALWSLPLYLESYLSWIRPSDRDAFLEEFRTSLPKSCDL

PTMs - O15287 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
K28 Ubiquitination
K182 Ubiquitination
K258 Ubiquitination
K347 Ubiquitination
S383 Phosphorylation P06493 (CDK1)
S387 Phosphorylation P06493 (CDK1)
K430 Ubiquitination
T487 Phosphorylation P06493 (CDK1)
K491 Ubiquitination
K504 Ubiquitination
T557 Phosphorylation
K559 Ubiquitination
K618 Ubiquitination

Research Backgrounds

Function:

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.

Subcellular Location:

Nucleus. Cytoplasm.
Note: The major form is nuclear. The minor form is cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis and thymus. Found in lymphoblasts.

Subunit Structure:

Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCA and FANCL, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. The complex with FANCC and FANCG may also include EIF2AK2 and HSP70. When phosphorylated at Ser-7, forms a complex with BRCA2, FANCD2 and XRCC3.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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