Phospho-DYN1 (Ser778) Antibody | Affinity Biosciences

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Product:	Phospho-DYN1 (Ser778) Antibody
Catalog:	AF0051
Description:	Rabbit polyclonal antibody to Phospho-DYN1 (Ser778)
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	100kDa; 97kD(Calculated).
Uniprot:	Q05193
RRID:	AB_2834096

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Phospho-DYN1 (Ser778) Antibody detects endogenous levels of DYN1 only when phosphorylated at Sersine 778.

RRID:

AB_2834096
Cite Format: Affinity Biosciences Cat# AF0051, RRID:AB_2834096.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B dynamin; D100; DNM 1; DNM; DNM1; DYN1_HUMAN; Dynamin; Dynamin-1; Dynamin1;

Immunogens

Immunogen:

Uniprot:

Q05193(DYN1_HUMAN) >>Visit HPA database.

Gene(ID):

DNM1(1759)

Description:

Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. This gene is located on chromosome 9. More than sixty highly conserved copies of the 3' region of this gene are found on other chromosomes, particularly chromosomes Y and 15. These copies are referred to as DNM1DN duplicons. Multiple splice variants exist for this gene but only two alternatively spliced transcripts encoding distinct isoforms have been described completely.

Sequence:

Q05193 DYN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLVLQLVNATTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGMTKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKVAKEVDPQGQRTIGVITKLDLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKVLNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVKKQVKKIREPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNHEDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAPPGVPSRSGQASPSRPESPRPPFDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Pig

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05193 As Substrate

Q05193

Site	PTM Type	Enzyme	Source
S46	Phosphorylation		Uniprot
S61	Phosphorylation		Uniprot
K113	Ubiquitination		Uniprot
S116	Phosphorylation		Uniprot
K206	Ubiquitination		Uniprot
T214	Phosphorylation		Uniprot
R217	Methylation		Uniprot
K223	Ubiquitination		Uniprot
Y231	Phosphorylation	P12931 (SRC)	Uniprot
K257	Ubiquitination		Uniprot
S261	Phosphorylation		Uniprot
S264	Phosphorylation		Uniprot
R271	Methylation		Uniprot
S302	Phosphorylation		Uniprot
S306	Phosphorylation		Uniprot
S347	Phosphorylation		Uniprot
Y354	Phosphorylation		Uniprot
K376	Ubiquitination		Uniprot
T411	Phosphorylation		Uniprot
S433	Phosphorylation		Uniprot
S437	Phosphorylation		Uniprot
T438	Phosphorylation		Uniprot
T443	Phosphorylation		Uniprot
S512	Phosphorylation		Uniprot
K523	Ubiquitination		Uniprot
K598	Acetylation		Uniprot
S668	Phosphorylation		Uniprot
Y669	Phosphorylation		Uniprot
Y732	Phosphorylation		Uniprot
S774	Phosphorylation		Uniprot
T776	Phosphorylation		Uniprot
S777	Phosphorylation		Uniprot
S778	Phosphorylation	P17252 (PRKCA)	Uniprot
T780	Phosphorylation	Q00535 (CDK5)	Uniprot
S795	Phosphorylation	Q13627 (DYRK1A)	Uniprot
S822	Phosphorylation		Uniprot
S851	Phosphorylation		Uniprot
S857	Phosphorylation	Q13627 (DYRK1A)	Uniprot

Research Backgrounds

Q05193_DYN1_HUMAN

Function:

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton.
Note: Microtubule-associated.

Subunit Structure:

Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 (By similarity). Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with SNX9. Interacts with MYO1E (via SH3 domain). Interacts with SNX33 (via SH3 domain). Interacts with UNC119; leading to a decrease of DNM1 GTPase activity (By similarity). Interacts with DIAPH1. Interacts with AMPH, BIN1 AND SYNJ1 (By similarity).

Family&Domains:

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

Restrictive clause

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For Research Use Only.
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Phospho-DYN1 (Ser778) Antibody - #AF0051