FLI1 Antibody - #AF0161

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Product: FLI1 Antibody
Catalog: AF0161
Description: Rabbit polyclonal antibody to FLI1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 50kDa; 51kD(Calculated).
Uniprot: Q01543
RRID: AB_2833342

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FLI1 Antibody detects endogenous levels of total FLI1.
RRID:
AB_2833342
Cite Format: Affinity Biosciences Cat# AF0161, RRID:AB_2833342.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ERGB transcription factor; Ewing Sarcoma breakpoint region 2; EWSR 2; EWSR2; FLI 1; FLI 1 proto oncogene; Fli-1 proto-oncogene, ETS transcription factor; FLI1; FLI1 EWS fusion gene; FLI1 proto oncogene; FLI1_HUMAN; Friend leukemia integration 1 (FLI1) transcription factor; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; Sarcoma breakpoint region 2 (EWSR2); SIC 1; SIC1; Transcription factor ERGB; Viral integration region FLI1; Viral integration region FLI1, mouse, homolog of;

Immunogens

Immunogen:
Uniprot:

Q01543(FLI1_HUMAN) >>Visit HPA database.

Gene(ID):
FLI1(2313)
Description:
FLI1 Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Belongs to the ETS family. Can form homodimers or heterodimers with ETV6/TEL1. 2 isoforms of the human protein are produced by alternative splicing.
Sequence:
MDGTIKEALSVVSDDQSLFDSAYGAAAHLPKADMTASGSPDYGQPHKINPLPPQQEWINQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01543 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S13 Phosphorylation
S21 Phosphorylation
T35 Phosphorylation
S37 Phosphorylation
S39 Phosphorylation
Y42 Phosphorylation
K47 Ubiquitination
K67 Sumoylation
Y70 Phosphorylation
S76 Phosphorylation
S79 Phosphorylation
S86 Phosphorylation
K87 Ubiquitination
S89 Phosphorylation
K172 Ubiquitination
K217 Ubiquitination
S221 Phosphorylation
Y222 Phosphorylation
S224 Phosphorylation
S236 Phosphorylation
K240 Acetylation
K240 Ubiquitination
S241 Phosphorylation
K252 Acetylation
Y263 Phosphorylation
T312 Phosphorylation Q05655 (PRKCD)
K325 Ubiquitination
Y332 Phosphorylation
K334 Ubiquitination
K350 Ubiquitination
K380 Acetylation
K380 Ubiquitination
K397 Acetylation
R438 Methylation
S450 Phosphorylation
Y451 Phosphorylation
Y452 Phosphorylation

Research Backgrounds

Function:

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Can form homodimers or heterodimers with ETV6/TEL1.

Family&Domains:

Belongs to the ETS family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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