MRGRD Antibody | Affinity Biosciences

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MRGRD Antibody - Western blot analysis of MRGRD expression in A431 whole cell lysates .

MRGRD Antibody - DF2820 at 1/100 staining human colorectal cancer by IHC-P.

Product:	MRGRD Antibody
Catalog:	DF2820
Description:	Rabbit polyclonal antibody to MRGRD
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	41 kDa; 36kD(Calculated).
Uniprot:	Q8TDS7
RRID:	AB_2840026

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

MRGRD Antibody detects endogenous levels of total MRGRD.

RRID:

AB_2840026
Cite Format: Affinity Biosciences Cat# DF2820, RRID:AB_2840026.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Beta alanine receptor; Beta-alanine receptor; G protein coupled receptor TGR7; G-protein coupled receptor TGR7; mas related G protein coupled MRGD; Mas related G protein coupled receptor member D; MAS related GPR member D; Mas-related G-protein coupled receptor member D; MRGD; Mrgprd; MRGRD_HUMAN; TGR7;

Immunogens

Immunogen:

Uniprot:

Q8TDS7(MRGRD_HUMAN) >>Visit HPA database.

Gene(ID):

MRGPRD(116512)

Description:

Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci.

Sequence:

Q8TDS7 MRGRD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNQTLNSSGTVESALNYSRGSTVHTAYLVLSSLAMFTCLCGMAGNSMVIWLLGFRMHRNPFCIYILNLAAADLLFLFSMASTLSLETQPLVNTTDKVHELMKRLMYFAYTVGLSLLTAISTQRCLSVLFPIWFKCHRPRHLSAWVCGLLWTLCLLMNGLTSSFCSKFLKFNEDRCFRVDMVQAALIMGVLTPVMTLSSLTLFVWVRRSSQQWRRQPTRLFVVVLASVLVFLICSLPLSIYWFVLYWLSLPPEMQVLCFSLSRLSSSVSSSANPVIYFLVGSRRSHRLPTRSLGTVLQQALREEPELEGGETPTVGTNEMGA

PTMs - Q8TDS7 As Substrate

Q8TDS7

Site	PTM Type	Enzyme	Source
Y17	Phosphorylation		Uniprot
S18	Phosphorylation		Uniprot

Research Backgrounds

Q8TDS7_MRGRD_HUMAN

Function:

May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin-stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Localized at the plasma membrane but internalized into the cytoplasm after treatment with beta-alanine.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Mas subfamily.

Research Fields

· Organismal Systems > Endocrine system > Renin-angiotensin system. (View pathway)

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MRGRD Antibody - #DF2820