F4/80 Antibody - #DF2789
(22)
(17)
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat
Prediction:
Mouse(%), Bovine(83%), Sheep(83%)
Clonality:
Polyclonal
Specificity:
F4/80 Antibody detects endogenous levels of total F4/80.
RRID:
AB_2839995
Cite Format: Affinity Biosciences Cat# DF2789, RRID:AB_2839995.
Cite Format: Affinity Biosciences Cat# DF2789, RRID:AB_2839995.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
ADGRE1; Adhesion G protein coupled receptor E1; Adhesion G protein-coupled receptor E1; AGRE1_HUMAN; Cell surface glycoprotein EMR1; Cell surface glycoprotein F4/80; DD7A5 7; Egf like module containing mucin like hormone receptor like 1; Egf like module containing mucin like hormone receptor like sequence 1; EGF like module receptor 1; EGF TM7; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGFTM7; EMR 1; EMR1 hormone receptor; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Description:
Could be involved in cell-cell interactions;
Sequence:
- Q14246 AGRE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRGFNLLLFWGCCVMHSWEGHIRPTRKPNTKGNNCRDSTLCPAYATCTNTVDSYYCACKQGFLSSNGQNHFKDPGVRCKDIDECSQSPQPCGPNSSCKNLSGRYKCSCLDGFSSPTGNDWVPGKPGNFSCTDINECLTSSVCPEHSDCVNSMGSYSCSCQVGFISRNSTCEDVDECADPRACPEHATCNNTVGNYSCFCNPGFESSSGHLSFQGLKASCEDIDECTEMCPINSTCTNTPGSYFCTCHPGFAPSNGQLNFTDQGVECRDIDECRQDPSTCGPNSICTNALGSYSCGCIAGFHPNPEGSQKDGNFSCQRVLFKCKEDVIPDNKQIQQCQEGTAVKPAYVSFCAQINNIFSVLDKVCENKTTVVSLKNTTESFVPVLKQISTWTKFTKEETSSLATVFLESVESMTLASFWKPSANITPAVRTEYLDIESKVINKECSEENVTLDLVAKGDKMKIGCSTIEESESTETTGVAFVSFVGMESVLNERFFKDHQAPLTTSEIKLKMNSRVVGGIMTGEKKDGFSDPIIYTLENIQPKQKFERPICVSWSTDVKGGRWTSFGCVILEASETYTICSCNQMANLAVIMASGELTMDFSLYIISHVGIIISLVCLVLAIATFLLCRSIRNHNTYLHLHLCVCLLLAKTLFLAGIHKTDNKMGCAIIAGFLHYLFLACFFWMLVEAVILFLMVRNLKVVNYFSSRNIKMLHICAFGYGLPMLVVVISASVQPQGYGMHNRCWLNTETGFIWSFLGPVCTVIVINSLLLTWTLWILRQRLSSVNAEVSTLKDTRLLTFKAFAQLFILGCSWVLGIFQIGPVAGVMAYLFTIINSLQGAFIFLIHCLLNGQVREEYKRWITGKTKPSSQSQTSRILLSSMPSASKTG
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Bovine
83
Sheep
83
Dog
67
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14246 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T50 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| Y736 | Phosphorylation | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| T770 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Subcellular Location:
Cell membrane>Multi-pass membrane protein.
Tissue Specificity:
Expression is restricted to eosinophils.
Family&Domains:
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
References
1). Zwitterionic hydrogel-coated heart valves with improved endothelialization and anti-calcification properties. Materials science & engineering-C, Materials for biological applications, 2021
(PubMed: 34474880)
[IF=7.9]
2). Xuanfei Baidu formula alleviates impaired mitochondrial dynamics and activated NLRP3 inflammasome by repressing NF-κB and MAPK pathways in LPS-induced ALI and inflammation models. Phytomedicine, 2023
(PubMed: 36423572)
[IF=7.9]
3). Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine–Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis. Cellular and Molecular Gastroenterology and Hepatology, 2022
(PubMed: 35202887)
[IF=7.2]
Application: IHC Species: Mice Sample:
Figure 1
LPS/D-GalN-induced ALF enhances apoptosis and ferroptosis. (A) The survival rate of WT mice treated with vehicle or LPS/D-GalN (LPS, 20 μg/kg; D-GalN, 700 mg/kg) (n = 10). (B) Representative pictures of WT mice treated with vehicle or LPS/D-GalN for 3, 5, and 6 hours (n = 10). (C) Serum levels of ALT and AST with or without LPS/D-GalN co-injection (n = 6). (D) H&E and TUNEL staining after LPS/D-GalN co-injection (n = 6). (E) Immunohistochemistry of TNFα and F4/80 staining after LPS/D-GalN co-injection (n = 6). (F) Transmission electron microscope of liver tissues (n = 3). (G) MDA assay of liver homogenates (n = 5). (H and J) Western blot analyses of TFR, DMT1, GPX4, and XCT from WT mice (H) with or without LPS/D-GalN and (I) quantitative results (n = 3–9). (I and K) FSP1, DHODH, and POR proteins. Western blot analyses of WT mice (I) with or without LPS/D-GalN and (K) quantitative results (n = 3–9). (L) Immunohistochemistry of DMT1 staining and immunofluorescence of Ptgs2 in livers with or without LPS/D-GalN (n = 6). (M) Quantitative results of DMT1 and Ptgs2. All data were obtained from WT mice. Scale bars: 100 μm. Data are presented as means ± SEM. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
4). Mammary Epithelial Cell-Derived Exosomal miR-221-3p Regulates Macrophage Polarization by Targeting Igf2bp2 during Mastitis. Journal of Agricultural and Food Chemistry, 2023
(PubMed: 37757458)
[IF=6.1]
5). Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus. Cells, 2022
(PubMed: 36359855)
[IF=6.0]
6). Caveolin-1 protects against DSS-induced colitis through inhibiting intestinal nitrosative stress and mucosal barrier damage in mice. BIOCHEMICAL PHARMACOLOGY, 2020
(PubMed: 32679126)
[IF=5.8]
Application: IHC Species: mouse Sample: colon
Fig. 6. |Pharmacologic Inhibition of iNOS Function Attenuates DSS Colitis. (a) Loss of body weight was recorded from day 0 to day 7. (b) Colon lengths were described and quantified in a bar graph. (c) Serum NO level was quantified in a bar graph. Immunohistochemistry analysis was utilized for the F4/80 (d) and Claudin-1 (e).
Application: IF/ICC Species: mouse Sample: colon
Fig. 2. |Down-regulation of Cav-1 in the colon tissue and accompany with the increase of INOS and NO levels after DSS administration. (a) Western blot analysis for detecting Cav-1, Bcl-2, P-Stat3 and Stat3 expression incolon tissues and quantified in a bar graph. (b) Immunofluorescence analysis for detecting Cav-1 and the macrophage-specific marker F4/80 expression in the colon tissue
7). Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury. Molecular medicine (Cambridge, Mass.), 2023
(PubMed: 37875838)
[IF=5.7]
Application: WB Species: Mouse Sample: kidney tissues
Fig. 5 CX3CL1 deficiency attenuated kidney inflammation and macrophage infiltration in cisplatin-induced model mice. A Western blotting was conducted to assess the expression profiles of IL-6, TNF-α, and F4/80 in kidney tissues. B, C Levels of IL-6 and TNF-α in mouse sera. D Image representations of TNF-α and F4/80 expression by immunohistochemistry. Scale bar = 20 μm. E podocytes were transfected with siRNA-CX3CL1 as indicated and treated with cisplatin (10 μM) for 24 h. CX3CL1, TNF-α, and IL-6 expression profiles were then measured by western blotting. F, G Levels of IL-6 and TNF-α in podocyte cell supernatant. H, I Representative micrographs of CX3CL1 and TNF-α staining in podocytes. Scale bar = 10 μm. (IL-6, interleukin 6; TNF-α, tumor necrosis factor-α; P value was calculated by one-way analysis of variance and Tukey’s test.
Application: IHC Species: Mouse Sample: kidney tissues
Fig. 5 CX3CL1 deficiency attenuated kidney inflammation and macrophage infiltration in cisplatin-induced model mice. A Western blotting was conducted to assess the expression profiles of IL-6, TNF-α, and F4/80 in kidney tissues. B, C Levels of IL-6 and TNF-α in mouse sera. D Image representations of TNF-α and F4/80 expression by immunohistochemistry. Scale bar = 20 μm. E podocytes were transfected with siRNA-CX3CL1 as indicated and treated with cisplatin (10 μM) for 24 h. CX3CL1, TNF-α, and IL-6 expression profiles were then measured by western blotting. F, G Levels of IL-6 and TNF-α in podocyte cell supernatant. H, I Representative micrographs of CX3CL1 and TNF-α staining in podocytes. Scale bar = 10 μm. (IL-6, interleukin 6; TNF-α, tumor necrosis factor-α; P value was calculated by one-way analysis of variance and Tukey’s test.
8). Extracts of Vine Tea Improve Diet-Induced Non-Alcoholic Steatohepatitis Through AMPK-LXRα Signaling. Frontiers in Pharmacology, 2021
(PubMed: 34393793)
[IF=5.6]
Application: IF/ICC Species: Mice Sample: liver tissue
FIGURE 4
VTE inhibits hepatic inflammation in MCDD-fed mice. Male C57BL/6J mice (20 ± 2 g) were fed an MCD diet in the presence and absence of VTE (1 g 100 g−1 diet) for 6 weeks (A) F4/80 immunofluorescence analysis in the livers from the MCS diet-, MCD diet- and MCD + VTE-fed mice. Scale bars, 50 μm (B) F4/80 mRNA levels in livers; n = 5 per group (C) ELISA analysis of serum TNFα levels; n = 5–6 per group (D) Relative mRNA expression of pro-inflammation genes in mouse liver; n = 3–5 per group. β-ACTIN was used as an internal control for normalizing the mRNA levels. Data are statistically analysed as means ± SEM. # p < 0.05, MCD compared with the MCS group of mice. *p < 0.05, MCD compared with the MCD + VTE group. NS, no significance.
9). Wu-Mei-Wan Ameliorates Murine Ulcerative Colitis by Regulating Macrophage Polarization. Frontiers in Pharmacology, 2022
(PubMed: 35387334)
[IF=5.6]
Application: IHC Species: mouse Sample: colitis
FIGURE 2 | Effect of WMW on DSS-induced acute colitis in mice.(E) Double immunofluorescent signals from F4/80 and TNF-α, F4/80 Immunohistochemistry in DSS-induced colitis tissue at different groups (original magnification, 400 ×). n = 7–10, means ± SEM.*p <0.05, **p < 0.01,***p < 0.001.
10). Prediction and verification of the active ingredients and potential targets of Erhuang Quzhi Granules on non-alcoholic fatty liver disease based on network pharmacology. Journal of Ethnopharmacology, 2023
(PubMed: 37023836)
[IF=5.4]
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