CHRM3 Antibody - #DF2781

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Product: CHRM3 Antibody
Catalog: DF2781
Description: Rabbit polyclonal antibody to CHRM3
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 85 kDa,; 66kD(Calculated).
Uniprot: P20309
RRID: AB_2839987

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
CHRM3 Antibody detects endogenous levels of total CHRM3.
RRID:
AB_2839987
Cite Format: Affinity Biosciences Cat# DF2781, RRID:AB_2839987.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AChR; ACM3_HUMAN; cholinergic receptor muscarinic 3; CHRM 3; CHRM3; EGBRS; HM 3; HM3; m3 muscarinic acetylcholine receptor; M3 muscarinic receptor; muscarinic 3; Muscarinic acetylcholine receptor M3; muscarinic cholinergic m3 receptor; muscarinic m3 receptor;

Immunogens

Immunogen:
Uniprot:

P20309(ACM3_HUMAN) >>Visit HPA database.

Gene(ID):
CHRM3(1131)
Description:
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.;
Sequence:
MTLHNNSTTSPLFPNISSSWIHSPSDAGLPPGTVTHFGSYNVSRAAGNFSSPDGTTDDPLGGHTVWQVVFIAFLTGILALVTIIGNILVIVSFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAETENFVHPTGSSRSCSSYELQQQSMKRSNRRKYGRCHFWFTTKSWKPSSEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSTILNSTKLPSSDNLQVPEEELGMVDLERKADKLQAQKSVDDGGSFPKSFSKLPIQLESAVDTAKTSDVNSSVGKSTATLPLSFKEATLAKRFALKTRSQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTFWNLGYWLCYINSTVNPVCYALCNKTFRTTFKMLLLCQCDKKKRRKQQYQQRQSVIFHKRAPEQAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20309 As Substrate

Site PTM Type Enzyme
S291 Phosphorylation
Y365 Phosphorylation
S366 Phosphorylation
S413 Phosphorylation
S457 Phosphorylation
S578 Phosphorylation

Research Backgrounds

Function:

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: Colocalizes with TMEM147 in the endoplasmic reticulum (ER) membrane. TMEM147 impairs its trafficking to the cell membrane leading to its retention in the ER membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; the dimers can form tetramers. Interacts with NALCN (By similarity). Interacts with TMEM147.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein. VETERINARY MICROBIOLOGY, 2022 (PubMed: 35810687) [IF=3.3]

Application: WB    Species: piglets    Sample: 3D4 cells

Fig. 5. Interference with CHRM3 protein inhibited the activation of the pathway caused by P1 virus. (A)Schematic diagram of shRNA and sequencing results. Red and green boxes indicate the interference sites of shRNA-1. (B)Real-time PCR analysis of CHRM3 mRNA expression in 3D4 cells transfected with shRNA-N (empty vector control), shRNA-1, or shRNA-2 at 24 h and 48 h post-transfection (n = 3). Immunoblot analysis of CHRM3 protein in CHRM3 knockdown 3D4 cells. The right panel is the intensity of the CHRM3 protein level normalized to that of β-actin (n = 3). The 3D4 cells were transfected with shRNA-N or shRNA-1 for 48 h and then infected with P1 (shRNA-1 +P1) or uninfected (shRNA-N or shRNA-1). Real-time PCR (D) and Western blot (E) were used to detect the expression of the main components of the pancreatic secretory pathway (CHRM3, Gq, PLC-β2, PRKCA, Rab3D, RhoA) at 48 h post-infection (n = 3). The gray value of the Western blot band (F) was analyzed by ImageJ software. Student's t-test was used to examine the differences between groups (∗P 

Application: IF/ICC    Species: Human    Sample: HEK293 cells

Fig. 3. Both the VP1 protein of the virus and the CHRM3 protein upregulate main components of the pancreatic secretory pathway. (A) Amplification of VP1 and CHRM3 genes and restriction digestion identification of recombinant plasmids. Western blot (B) and Immunofluorescence (C) analysis of the expression of VP1 and CHRM3 protein in HEK293 cells transfected with pcDNA3.1-HA-CHRM3 or pcDNA3.1-VP1-Flag at 48 h post-transfection. (D) Western blot analysis of the expression of VP1 and CHRM3 proteins in 3D4 cells transfected with pcDNA3.1-HA-CHRM3 or pcDNA3.1-VP1-Flag at 24 h, 48 h, and 72 h post-transfection. The right panel is the intensity of the CHRM3 protein level normalized to that of β-actin (n = 3). (E) Real-time PCR analysis of CHRM3, Gq, PLC-β2, PRKCA, Rab3D, RhoA, Rac1, and amyA mRNA expression levels in 3D4 cells transfected with pcDNA3.1-VP1-Flag, pcDNA3.1-HA-CHRM3, or pcDNA 3.1 vector (control) at 24 h, 48 h, or 72 h post-transfection (n = 3). The differences between groups were analyzed by Student's t-test (∗P 

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