Somatostatin Receptor 5 Antibody | Affinity Biosciences

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Western blot analysis of SS5R expression in HEK293 cells .

Product:	Somatostatin Receptor 5 Antibody
Catalog:	DF2780
Description:	Rabbit polyclonal antibody to Somatostatin Receptor 5
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.:	42 kDa; 39kD(Calculated).
Uniprot:	P35346
RRID:	AB_2839986

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Zebrafish(89%), Bovine(91%), Horse(91%), Sheep(91%), Dog(91%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

Somatostatin Receptor 5 Antibody detects endogenous levels of total Somatostatin Receptor 5.

RRID:

AB_2839986
Cite Format: Affinity Biosciences Cat# DF2780, RRID:AB_2839986.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

SSTR5; Somatostatin receptor subtype 5; Somatostatin receptor type 5; SS 5R; SS-5-R; SS5 R; SS5-R; SS5R; SSR5_HUMAN; SST R5; SSTR 5; Sstr5;

Immunogens

Immunogen:

Uniprot:

P35346(SSR5_HUMAN) >>Visit HPA database.

Gene(ID):

SSTR5(6755)

Expression:

P35346 SSR5_HUMAN:

Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.

Description:

Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Sequence:

P35346 SSR5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPLFPASTPSWNASSPGAASGGGDNRTLVGPAPSAGARAVLVPVLYLLVCAAGLGGNTLVIYVVLRFAKMKTVTNIYILNLAVADVLYMLGLPFLATQNAASFWPFGPVLCRLVMTLDGVNQFTSVFCLTVMSVDRYLAVVHPLSSARWRRPRVAKLASAAAWVLSLCMSLPLLVFADVQEGGTCNASWPEPVGLWGAVFIIYTAVLGFFAPLLVICLCYLLIVVKVRAAGVRVGCVRRRSERKVTRMVLVVVLVFAGCWLPFFTVNIVNLAVALPQEPASAGLYFFVVILSYANSCANPVLYGFLSDNFRQSFQKVLCLRKGSGAKDADATEPRPDRIRQQQEATPPAHRAAANGLMQTSKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Zebrafish

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35346 As Substrate

P35346

Site	PTM Type	Source
R67	Methylation	Uniprot
S146	Phosphorylation	Uniprot
S147	Phosphorylation	Uniprot
S242	Phosphorylation	Uniprot
T247	Phosphorylation	Uniprot
T333	Phosphorylation	Uniprot
T347	Phosphorylation	Uniprot
K363	Ubiquitination	Uniprot

Research Backgrounds

P35346_SSR5_HUMAN

Function:

Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Increases cell growth inhibition activity of SSTR2 following heterodimerization.

PTMs:

Palmitoylated by ZDHHC5, but not ZDHHC3, nor ZDHHC8. Palmitoylation creates an additional intracellular loop which is thought to be important for efficient coupling to G-proteins and may target the protein to lipid rafts.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.

Subunit Structure:

Heterodimer with SSTR2. Heterodimerization with SSTR2 increases cell growth inhibition activity of SSTR2.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

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Somatostatin Receptor 5 Antibody - #DF2780