GPR92/GPR93 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of GPR92/GPR93 expression in A431 whole cell lysates .

Product:	GPR92/GPR93 Antibody
Catalog:	DF2774
Description:	Rabbit polyclonal antibody to GPR92/GPR93
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Bovine
Mol.Wt.:	Predicted band size : 41 kDa,Observed band size : 41 kDa; 41kD(Calculated).
Uniprot:	Q9H1C0
RRID:	AB_2839980

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(80%)

Clonality:

Polyclonal

Specificity:

GPR92/GPR93 Antibody detects endogenous levels of total GPR92/GPR93.

RRID:

AB_2839980
Cite Format: Affinity Biosciences Cat# DF2774, RRID:AB_2839980.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G protein coupled receptor 92; G-protein coupled receptor 92; G-protein coupled receptor 93; GPR 92; GPR 93; GPR92; GPR93; KPG 010; LPA receptor 5; LPA-5; Lpar5; LPAR5_HUMAN; Lysophosphatidic acid receptor 5; Probable G protein coupled receptor 92; Putative G protein coupled receptor 92;

Immunogens

Immunogen:

Uniprot:

Q9H1C0(LPAR5_HUMAN) >>Visit HPA database.

Gene(ID):

LPAR5(57121)

Expression:

Q9H1C0 LPAR5_HUMAN:

Not expressed in frontal cortex, basal forebrain, caudate putamen, thalamus, or hippocampus.

Description:

Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities.

Sequence:

Q9H1C0 LPAR5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLANSSSTNSSVLPCPDYRPTHRLHLVVYSLVLAAGLPLNALALWVFLRALRVHSVVSVYMCNLAASDLLFTLSLPVRLSYYALHHWPFPDLLCQTTGAIFQMNMYGSCIFLMLINVDRYAAIVHPLRLRHLRRPRVARLLCLGVWALILVFAVPAARVHRPSRCRYRDLEVRLCFESFSDELWKGRLLPLVLLAEALGFLLPLAAVVYSSGRVFWTLARPDATQSQRRRKTVRLLLANLVIFLLCFVPYNSTLAVYGLLRSKLVAASVPARDRVRGVLMVMVLLAGANCVLDPLVYYFSAEGFRNTLRGLGTPHRARTSATNGTRAALAQSERSAVTTDATRPDAASQGLLRPSDSHSLSSFTQCPQDSAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Pig

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H1C0 As Substrate

Q9H1C0

Site	PTM Type	Source
Y29	Phosphorylation	Uniprot
S268	Phosphorylation	Uniprot
S332	Phosphorylation	Uniprot

Research Backgrounds

Q9H1C0_LPAR5_HUMAN

Function:

Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Not expressed in frontal cortex, basal forebrain, caudate putamen, thalamus, or hippocampus.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

GPR92/GPR93 Antibody - #DF2774