GPR81 Antibody - #DF2766

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Product: GPR81 Antibody
Catalog: DF2766
Description: Rabbit polyclonal antibody to GPR81
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Dog
Mol.Wt.: 39 kDa; 39kD(Calculated).
Uniprot: Q9BXC0
RRID: AB_2839972

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(83%), Dog(100%)
Clonality:
Polyclonal
Specificity:
GPR81 Antibody detects endogenous levels of total GPR81.
RRID:
AB_2839972
Cite Format: Affinity Biosciences Cat# DF2766, RRID:AB_2839972.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FKSG80; FKSG80 protein; G protein coupled receptor 104; G protein coupled receptor 81; G protein coupled receptor GPR81; G-protein coupled receptor 104; G-protein coupled receptor 81; GPR 104; GPR 81; GPR104; GPR81; GPR81_HUMAN; HCA1; HCAR1; hydroxy-carboxylic acid receptor 1; Hydroxycarboxylic acid receptor 1; LACR1; lactate receptor 1; Putative chemokine receptor; T cell activation G protein coupled receptor; TA GPCR;

Immunogens

Immunogen:
Uniprot:

Q9BXC0(HCAR1_HUMAN) >>Visit HPA database.

Gene(ID):
HCAR1(27198)
Expression:
Q9BXC0 HCAR1_HUMAN:

Expressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus.

Description:
Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.
Sequence:
MYNGSCCRIEGDTISQVMPPLLIVAFVLGALGNGVALCGFCFHMKTWKPSTVYLFNLAVADFLLMICLPFRTDYYLRRRHWAFGDIPCRVGLFTLAMNRAGSIVFLTVVAADRYFKVVHPHHAVNTISTRVAAGIVCTLWALVILGTVYLLLENHLCVQETAVSCESFIMESANGWHDIMFQLEFFMPLGIILFCSFKIVWSLRRRQQLARQARMKKATRFIMVVAIVFITCYLPSVSARLYFLWTVPSSACDPSVHGALHITLSFTYMNSMLDPLVYYFSSPSFPKFYNKLKICSLKPKQPGHSKTQRPEEMPISNLGRRSCISVANSFQSQSDGQWDPHIVEWH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
83
Bovine
67
Sheep
67
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

References

1). Lactate as a metabolite from probiotic Lactobacilli mitigates ethanol-induced gastric mucosal injury: an in vivo study. BMC Complementary and Alternative Medicine, 2021 (PubMed: 33430871)

Application: IHC    Species: mice    Sample: gastric tissues

Fig. 6 Expression of lactate receptor GPR81 in the various groups (n = 4 per group). a Total RNA in gastric tissues was extracted and qPCR was carried out to examine the expression of genes encoding GPR81. C: Control group; D: disease group; L: Group treated with lactate at a dose of 3 g/ kg. The data were expressed as the means ± SD (n = 6). ** p < 0.01. b IHC staining for GPR81. Gastric samples were fixed and sectioned for staining by the primary antibody of GPR81. Brown granules in cells were considered as positive results

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