Product: FPRL1 Antibody
Catalog: DF2719
Description: Rabbit polyclonal antibody to FPRL1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Rat, Rabbit
Mol.Wt.: 38 kDa; 39kD(Calculated).
Uniprot: P25090
RRID: AB_2839925

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Rat(%), Rabbit(83%)
Clonality:
Polyclonal
Specificity:
FPRL1 Antibody detects endogenous levels of total FPRL1.
RRID:
AB_2839925
Cite Format: Affinity Biosciences Cat# DF2719, RRID:AB_2839925.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALXR; FMLP-R-I; FMLP-R-II; FMLP-related receptor I; FMLPX; Formyl peptide receptor 2; Formyl peptide receptor related; Formyl peptide receptor-like 1; FPR2; FPR2_HUMAN; FPR2A; FPRH1; FPRH2; FPRL1; HM63; Lipoxin A4 receptor; LXA4 receptor; LXA4R; N-formyl peptide receptor 2; RFP;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P25090 FPR2_HUMAN:

Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.

Description:
Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).;
Sequence:
METNFSTPLNEYEEVSYESAGYTVLRILPLVVLGVTFVLGVLGNGLVIWVAGFRMTRTVTTICYLNLALADFSFTATLPFLIVSMAMGEKWPFGWFLCKLIHIVVDINLFGSVFLIGFIALDRCICVLHPVWAQNHRTVSLAMKVIVGPWILALVLTLPVFLFLTTVTIPNGDTYCTFNFASWGGTPEERLKVAITMLTARGIIRFVIGFSLPMSIVAICYGLIAAKIHKKGMIKSSRPLRVLTAVVASFFICWFPFQLVALLGTVWLKEMLFYGKYKIIDILVNPTSSLAFFNSCLNPMLYVFVGQDFRERLIHSLPTSLERALSEDSAPTNDTAANSASPPAETELQAM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
83
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25090 As Substrate

Site PTM Type Enzyme
T196 Phosphorylation
S236 Phosphorylation
S237 Phosphorylation
Y302 Phosphorylation
S316 Phosphorylation
T319 Phosphorylation
S329 Phosphorylation
T332 Phosphorylation
T335 Phosphorylation

Research Backgrounds

Function:

Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophil chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). Receptor for the chemokine-like protein FAM19A5, mediating FAM19A5-stimulated macrophage chemotaxis and the inhibitory effect on TNFSF11/RANKL-induced osteoclast differentiation (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Associates with Amyloid-beta protein 42, product of APP, at the cell surface and the complex is then rapidly internalized.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.

Subunit Structure:

Interacts with Amyloid-beta protein 42, product of APP; the interaction takes place at the cell surface and the complex is then rapidly internalized.

(Microbial infection) Interacts with Staphylococcus aureus protein SSL13; this interaction leads to the activation of neutrophils.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

References

1). Lipoxin A4 regulates M1/M2 macrophage polarization via FPR2–IRF pathway. INFLAMMOPHARMACOLOGY (PubMed: 35235107) [IF=5.8]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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