HTR3E Antibody | Affinity Biosciences

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FIGURE 2 Bilirubin increased 5-HT3A receptor expression and neuron activities in the spinal cord.

Product:	HTR3E Antibody
Catalog:	DF2708
Description:	Rabbit polyclonal antibody to HTR3E
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	51kDa; 51kD(Calculated).
Uniprot:	A5X5Y0
RRID:	AB_2839914

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

HTR3E Antibody detects endogenous levels of total HTR3E.

RRID:

AB_2839914
Cite Format: Affinity Biosciences Cat# DF2708, RRID:AB_2839914.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5-HT-3; 5-HT3 receptor subunit E splice variant HTR3Ea; 5-HT3c1; 5-HT3E; 5-HT3R; 5-hydroxytryptamine (serotonin) receptor 3, family member E; 5-hydroxytryptamine receptor 3 subunit E; 5-hydroxytryptamine receptor 3E; 5HT3c1; 5HT3E; 5HT3R; HTR3; MGC120035; MGC120036; MGC120037; SEROTONIN 5-HT-3E RECEPTOR; serotonin receptor 3 subunit E; Serotonin receptor 3E;

Immunogens

Immunogen:

Uniprot:

A5X5Y0(5HT3E_HUMAN) >>Visit HPA database.

Gene(ID):

HTR3E(285242)

Expression:

A5X5Y0 5HT3E_HUMAN:

Expressed in adult colon and intestine.

Sequence:

A5X5Y0 5HT3E_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEGSWFHRKRFSFYLLLGFLLQGRGVTFTINCSGFGQHGADPTALNSVFNRKPFRPVTNISVPTQVNISFAMSAILDVNEQLHLLSSFLWLEMVWDNPFISWNPEECEGITKMSMAAKNLWLPDIFIIELMDVDKTPKGLTAYVSNEGRIRYKKPMKVDSICNLDIFYFPFDQQNCTLTFSSFLYTVDSMLLDMEKEVWEITDASRNILQTHGEWELLGLSKATAKLSRGGNLYDQIVFYVAIRRRPSLYVINLLVPSGFLVAIDALSFYLPVKSGNRVPFKITLLLGYNVFLLMMSDLLPTSGTPLIGVYFALCLSLMVGSLLETIFITHLLHVATTQPPPLPRWLHSLLLHCNSPGRCCPTAPQKENKGPGLTPTHLPGVKEPEVSAGQMPGPAEAELTGGSEWTRAQREHEAQKQHSVELWLQFSHAMDAMLFRLYLLFMASSIITVICLWNT

PTMs - A5X5Y0 As Substrate

A5X5Y0

Site	PTM Type	Enzyme	Source
S228	Phosphorylation		Uniprot

Research Backgrounds

A5X5Y0_5HT3E_HUMAN

Function:

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.

Tissue Specificity:

Expressed in adult colon and intestine.

Subunit Structure:

Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex.

Family&Domains:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3E sub-subfamily.

Research Fields

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

References

1). Bilirubin Induces Pain Desensitization in Cholestasis by Activating 5-Hydroxytryptamine 3A Receptor in Spinal Cord. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 33869168) [IF=5.5]

Application: WB Species: Rat Sample: HEK293 cells

FIGURE 2 Bilirubin increased 5-HT3A receptor expression and neuron activities in the spinal cord. (A) 5-HT3A receptor expression increased gradually in spinal cord enlargement of BDL rats, whereas expression of 5-HT3B, 5-HT3C, 5-HT3D, and 5-HT3E receptors did not change significantly. (B) The quantitative analysis of 5-HT3A receptor expression in BDL rats. (C) Intrathecal administration of bilirubin increased 5-HT3A receptor expression without changing other subtypes. (D) The quantitative analysis of 5-HT3A receptor expression after intrathecal administration of bilirubin in normal rats. (E) SDHNs cultured with bilirubin showed increased 5-HT3A receptor expression without changing other subtypes. (F) The quantitative analysis of 5-HT3A receptor expression in SDHNs. (G) Immunofluorescence showed that 5-HT3A receptor expression increased in laminas I and II in the spinal cords of BDL rats. The increased C-Fos protein (marker of neuron activity) expression indicated that neuron activities were also enhanced in the spinal cords of BDL rats. *P < 0.05.

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HTR3E Antibody - #DF2708