Nkx2.5 Antibody | Affinity Biosciences

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Product:	Nkx2.5 Antibody
Catalog:	DF2699
Description:	Rabbit polyclonal antibody to Nkx2.5
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	50 kDa; 35kD(Calculated).
Uniprot:	P52952
RRID:	AB_2839905

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Horse(82%), Sheep(91%), Dog(82%)

Clonality:

Polyclonal

Specificity:

Nkx2.5 Antibody detects endogenous levels of total Nkx2.5.

RRID:

AB_2839905
Cite Format: Affinity Biosciences Cat# DF2699, RRID:AB_2839905.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

NKX2.5, mouse, homolog of; Cardiac-specific homeobox 1; Cardiac-specific homeobox; CHNG5; CSX; CSX1; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; HLHS2; Homeobox protein CSX; Homeobox protein NK-2 homolog E; Homeobox protein Nkx 2.5; Homeobox protein Nkx-2.5; NK2 homeobox 5; NK2 transcription factor related locus 5; NK2 transcription factor related, locus 5 (Drosophila); NK2, Drosophila, homolog of, E; NKX2-5; NKX2.5; NKX25_HUMAN; Nkx2E; NKX4-1; Tinman paralog; VSD3;

Immunogens

Immunogen:

Uniprot:

P52952(NKX25_HUMAN) >>Visit HPA database.

Gene(ID):

NKX2-5(1482)

Expression:

P52952 NKX25_HUMAN:

Expressed only in the heart.

Description:

Receptor for VEGFC. Has a tyrosine-protein kinase activity.;

Sequence:

P52952 NKX25_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKELCALQKAVELEKTEADNAERPRARRRRKPRVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELVGLPPPPPPPARRIAVPVLVRDGKPCLGDSAPYAPAYGVGLNPYGYNAYPAYPGYGGAACSPGYSCTAAYPAGPSPAQPATAAANNNFVNFGVGDLNAVQSPGIPQSNSGVSTLHGIRAW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Horse

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52952 As Substrate

P52952

Site	PTM Type	Source
S78	Phosphorylation	Uniprot
S164	Phosphorylation	Uniprot
T180	Phosphorylation	Uniprot
R225	Methylation	Uniprot

Research Backgrounds

P52952_NKX25_HUMAN

Function:

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). Binds to the core DNA motif of NPPA promoter. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.

Subcellular Location:

Nucleus.

Tissue Specificity:

Expressed only in the heart.

Subunit Structure:

Homodimer (via the homeobox); binds DNA as homodimer. Interacts (via the homeobox) with TBX5 (via the T-box); this complex binds DNA. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Interacts with TBX18 (By similarity).

Family&Domains:

The homeobox domain binds to double-stranded DNA (PubMed:22849347).

Belongs to the NK-2 homeobox family.

References

1). Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro. PLoS One, 2015 (PubMed: 25898012) [IF=3.7]

Application: WB Species: Sample: miR-20b cells

Fig 7. |Effects of miR-20b on the BMP signaling pathway, as assessed by by western blotting. a): in the miR-20b overexpression group; b): in miR-20b silenced group. (n = 3, *: P<0.05, **: P<0.01, ***: P<0.001)

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Nkx2.5 Antibody - #DF2699