BECN1L1 Antibody | Affinity Biosciences

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Western blot analysis of BECN1L1 expression in HEK293 cells.

DF2696 at 1/100 staining Human gastric tissue by IHC-P.

Product:	BECN1L1 Antibody
Catalog:	DF2696
Description:	Rabbit polyclonal antibody to BECN1L1
Application:	WB IHC
Reactivity:	Human
Mol.Wt.:	48kDa; 48kD(Calculated).
Uniprot:	A8MW95
RRID:	AB_2839902

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

BECN1L1 Antibody detects endogenous levels of total BECN1L1.

RRID:

AB_2839902
Cite Format: Affinity Biosciences Cat# DF2696, RRID:AB_2839902.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

A8MW95(BECN2_HUMAN) >>Visit HPA database.

Gene(ID):

BECN2(441925)

Expression:

A8MW95 BECN2_HUMAN:

Present in fetal and adult brain (at protein level).

Sequence:

A8MW95 BECN2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSIRFLCQRCHQALKLSGSSESRSLPAAPAPTSGQAEPGDTREPGVTTREVTDAEEQQDGASSRSPPGDGSVSKGHANIFTLLGELGAMHMLSSIQKAAGDIFDIVSGQAVVDHPLCEECTDSLLEQLDIQLALTEADSQNYQRCLETGELATSEDEAAALRAELRDLELEEARLVQELEDVDRNNARAAADLQAAQAEAAELDQQERQHYRDYSALKRQQLELLDQLGNVENQLQYARVQRDRLKEINCFTATFEIWVEGPLGVINNFRLGRLPTVRVGWNEINTAWGQAALLLLTLANTIGLQFQRYRLIPCGNHSYLKSLTDDRTELPLFCYGGQDVFLNNKYDRAMVAFLDCMQQFKEEAEKGELGLSLPYGIQVETGLMEDVGGRGECYSIRTHLNTQELWTKALKFMLINFKWSLIWVASRYQK

PTMs - A8MW95 As Substrate

A8MW95

Site	PTM Type	Enzyme	Source
Y347	Phosphorylation		Uniprot

Research Backgrounds

A8MW95_BECN2_HUMAN

Function:

Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Present in fetal and adult brain (at protein level).

Subunit Structure:

Homodimer (via coiled-coil domain). Interacts (via coiled-coil domain) with ATG14 (via coiled-coil domain); this interaction is tighter than BECN2 self-association. Interacts with AMBRA1, UVRAG and PIK3C3/VPS34; these interactions are not disrupted by starvation. Does not interact with RUBCN. Interacts (via N-terminus) with GPRASP1/GASP1; the interaction is direct.

Family&Domains:

Belongs to the beclin family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

BECN1L1 Antibody - #DF2696