Product: PRKCH Antibody
Catalog: DF2675
Description: Rabbit polyclonal antibody to PRKCH
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Chicken, Xenopus
Mol.Wt.: 78 kDa; 78kD(Calculated).
Uniprot: P24723
RRID: AB_2839881

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
PRKCH Antibody detects endogenous levels of total PRKCH.
RRID:
AB_2839881
Cite Format: Affinity Biosciences Cat# DF2675, RRID:AB_2839881.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KPCL_HUMAN; MGC 5363; MGC26269; MGC5363; nPKC eta; nPKC-eta; PKC h; PKC L; PKC-L; PKCh; PKCL; Prkch; PRKCL; Protein kinase C eta; Protein kinase C eta type;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P24723 KPCL_HUMAN:

Most abundant in lung, less in heart and skin.

Description:
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.;PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.;
Sequence:
MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQINGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDSKIAEQRFGINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKESSKEGNGIGVNSSNRLGIDNFEFIRVLGKGSFGKVMLARVKETGDLYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHDKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHAILRHPFFKEIDWAQLNHRQIEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Xenopus
82
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24723 As Substrate

Site PTM Type Enzyme
T25 Phosphorylation
S28 Phosphorylation P24723 (PRKCH)
S32 Phosphorylation P24723 (PRKCH)
K36 Ubiquitination
Y94 Phosphorylation
K152 Ubiquitination
T183 Phosphorylation
K234 Ubiquitination
S249 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
K321 Ubiquitination
S324 Phosphorylation
S326 Phosphorylation
K337 Ubiquitination
K363 Ubiquitination
S365 Phosphorylation
K375 Ubiquitination
K384 Ubiquitination
K388 Ubiquitination
K404 Ubiquitination
K481 Ubiquitination
K494 Ubiquitination
T513 Phosphorylation
K591 Ubiquitination
S599 Phosphorylation
T601 Phosphorylation
K615 Ubiquitination
T656 Phosphorylation
S675 Phosphorylation
Y676 Phosphorylation

PTMs - P24723 As Enzyme

Substrate Site Source
P04083 (ANXA1) S27 Uniprot
P05107 (ITGB2) S745 Uniprot
P05107 (ITGB2) T758 Uniprot
P09211 (GSTP1) S43 Uniprot
P09211 (GSTP1) S185 Uniprot
P24723 (PRKCH) S28 Uniprot
P24723 (PRKCH) S32 Uniprot
P29474 (NOS3) T495 Uniprot
P49840 (GSK3A) S21 Uniprot
P49841 (GSK3B) S9 Uniprot
Q04206 (RELA) S536 Uniprot
Q06124-2 (PTPN11) S576 Uniprot
Q06124 (PTPN11) S580 Uniprot
Q06124 (PTPN11) S595 Uniprot
Q15139 (PRKD1) S738 Uniprot
Q15139 (PRKD1) S742 Uniprot
Q16625 (OCLN) T403 Uniprot
Q16625 (OCLN) T404 Uniprot
Q9BZL6 (PRKD2) S706 Uniprot
Q9BZL6 (PRKD2) S710 Uniprot
Q9BZL6 (PRKD2) S876 Uniprot

Research Backgrounds

Function:

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Most abundant in lung, less in heart and skin.

Subunit Structure:

Interacts with FYN and RALA (By similarity). Interacts with DGKQ.

Family&Domains:

The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Research Fields

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

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