POMC Antibody - #DF2655

(1)   (2)  
Product: POMC Antibody
Catalog: DF2655
Description: Rabbit polyclonal antibody to POMC
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Dog
Mol.Wt.: 29 kDa; 29kD(Calculated).
Uniprot: P01189
RRID: AB_2839861

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
POMC Antibody detects endogenous levels of total POMC.
RRID:
AB_2839861
Cite Format: Affinity Biosciences Cat# DF2655, RRID:AB_2839861.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACTH; Adrenocorticotropic hormone; Adrenocorticotropin; Alpha melanocyte stimulating hormone; alpha-MSH; alphaMSH; Beta LPH; Beta melanocyte stimulating hormone; Beta-endorphin; beta-MSH; CLIP; Corticotropin; Corticotropin lipotropin; Corticotropin-like intermediary peptide; Gamma LPH; gamma-MSH; Lipotropin beta; Lipotropin gamma; Lipotropin, included; LPH; Melanocyte-stimulating hormone, included; Melanotropin alpha; Melanotropin beta; Melanotropin gamma; Melanotropin, included; Met-enkephalin; MSH; NPP; Opiomelanocortin prepropeptide; POC; POMC; Pomc-1; Pomc1; Pomc2; Pro ACTH endorphin; Pro opiomelanocortin; Pro-opiomelanocortin-alpha; Proopiomelanocortin; Proopiomelanocortin preproprotein; Tetracosactide;

Immunogens

Immunogen:
Uniprot:

P01189(COLI_HUMAN) >>Visit HPA database.

Gene(ID):
POMC(5443)
Expression:
P01189 COLI_HUMAN:

ACTH and MSH are produced by the pituitary gland.

Sequence:
MPRSCCSRSGALLLALLLQASMEVRGWCLESSQCQDLTTESNLLECIRACKPDLSAETPMFPGNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSGSSGAGQKREDVSAGEDCGPLPEGGPEPRSDGAKPGPREGKRSYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEFKRELTGQRLREGDGPDGPADDGAGAQADLEHSLLVAAEKKDEGPYRMEHFRWGSPPKDKRYGGFMTSEKSQTPLVTLFKNAIIKNAYKKGE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
88
Sheep
88
Dog
88
Pig
75
Chicken
75
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01189 As Substrate

Site PTM Type Enzyme
T71 O-Glycosylation
S92 Phosphorylation
S95 Phosphorylation
S97 Phosphorylation
S108 Phosphorylation
S168 Phosphorylation
S230 Phosphorylation

Research Backgrounds

Function:

Stimulates the adrenal glands to release cortisol.

Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes.

Increases the pigmentation of skin by increasing melanin production in melanocytes.

Endogenous orexigenic opiate.

Endogenous opiate.

PTMs:

Specific enzymatic cleavages at paired basic residues yield the different active peptides.

O-glycosylated; reducing sugar is probably N-acetylgalactosamine.

Subcellular Location:

Secreted.
Note: Melanocyte-stimulating hormone alpha and beta-endorphin are stored in separate granules in hypothalamic POMC neurons, suggesting that secretion may be under the control of different regulatory mechanisms.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

ACTH and MSH are produced by the pituitary gland.

Family&Domains:

Belongs to the POMC family.

Research Fields

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

References

1). Sodium tanshinone IIA sulfonate suppresses microglia polarization and neuroinflammation possibly via regulating miR-125b-5p/STAT3 axis to ameliorate neuropathic pain. European journal of pharmacology, 2024 (PubMed: 38552937) [IF=5.0]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.