BAG2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat brain, using BAG2 Antibody.

Product:	BAG2 Antibody
Catalog:	DF2650
Description:	Rabbit polyclonal antibody to BAG2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	24 kDa; 24kD(Calculated).
Uniprot:	O95816
RRID:	AB_2839856

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(90%), Sheep(90%), Rabbit(100%), Dog(80%)

Clonality:

Polyclonal

Specificity:

BAG2 Antibody detects endogenous levels of total BAG2.

RRID:

AB_2839856
Cite Format: Affinity Biosciences Cat# DF2650, RRID:AB_2839856.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BAG 2; BAG family molecular chaperone regulator 2; BAG-2; BAG-family molecular chaperone regulator-2; Bag2; BAG2_HUMAN; BCL 2 associated athanogene 2; Bcl-2-associated athanogene 2; BCL2 associated athanogene 2; BCL2-associated athanogene 2; dJ417I1.2 (BAG family molecular chaperone regulator 2); dJ417I1.2; KIAA0576; MGC149462; OTTHUMP00000016668;

Immunogens

Immunogen:

Uniprot:

O95816(BAG2_HUMAN) >>Visit HPA database.

Gene(ID):

BAG2(9532)

Description:

Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.;

Sequence:

O95816 BAG2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQAKINAKANEGRFCRSSSMADRSSRLLESLDQLELRVEALREAATAVEQEKEILLEMIHSIQNSQDMRQISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQQESLKHATRIIDEVVNKFLDDLGNAKSHLMSLYSACSSEVPHGPVDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSKTLQQNAESRFN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Bovine

Sheep

Dog

Horse

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95816 As Substrate

O95816

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
K5	Ubiquitination		Uniprot
K9	Ubiquitination		Uniprot
S20	Phosphorylation	P49137 (MAPKAPK2)	Uniprot
S31	Phosphorylation		Uniprot
S73	Phosphorylation		Uniprot
K111	Acetylation		Uniprot
K111	Ubiquitination		Uniprot
K123	Ubiquitination		Uniprot
S133	Phosphorylation		Uniprot
S137	Phosphorylation		Uniprot
Y139	Phosphorylation		Uniprot
S140	Phosphorylation		Uniprot
S143	Phosphorylation		Uniprot
S144	Phosphorylation		Uniprot
K154	Ubiquitination		Uniprot
C162	S-Nitrosylation		Uniprot
K168	Acetylation		Uniprot
K168	Ubiquitination		Uniprot
K169	Acetylation		Uniprot
T176	Phosphorylation		Uniprot
K186	Acetylation		Uniprot
K186	Ubiquitination		Uniprot
K189	Ubiquitination		Uniprot
K195	Ubiquitination		Uniprot
K200	Ubiquitination		Uniprot
T201	Phosphorylation		Uniprot

Research Backgrounds

O95816_BAG2_HUMAN

Function:

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.

Subunit Structure:

Binds to the ATPase domain of HSP/HSC70 chaperones. May interact with NWD1. Interacts with HSPA1A (via NBD), HSPA1B (via NBD) and HSPA8.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

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BAG2 Antibody - #DF2650