RASA2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse heart, using RASA2 Antibody.

Product:	RASA2 Antibody
Catalog:	DF2647
Description:	Rabbit polyclonal antibody to RASA2
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	96kDa; 97kD(Calculated).
Uniprot:	Q15283
RRID:	AB_2839853

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

RASA2 Antibody detects endogenous levels of total RASA2.

RRID:

AB_2839853
Cite Format: Affinity Biosciences Cat# DF2647, RRID:AB_2839853.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GAP1m; GTPase activating protein of RAS; Ras GTPase activating protein 2; Ras GTPase-activating protein 2; RAS p21 protein activator 2; Rasa2; RASA2_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q15283(RASA2_HUMAN) >>Visit HPA database.

Gene(ID):

RASA2(5922)

Description:

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4).

Sequence:

Q15283 RASA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAAAPAAAAASSEAPAASATAEPEAGDQDSREVRVLQSLRGKICEAKNLLPYLGPHKMRDCFCTINLDQEEVYRTQVVEKSLSPFFSEEFYFEIPRTFQYLSFYVYDKNVLQRDLRIGKVAIKKEDLCNHSGKETWFSLQPVDSNSEVQGKVHLELKLNELITENGTVCQQLVVHIKACHGLPLINGQSCDPYATVSLVGPSRNDQKKTKVKKKTSNPQFNEIFYFEVTRSSSYTRKSQFQVEEEDIEKLEIRIDLWNNGNLVQDVFLGEIKVPVNVLRTDSSHQAWYLLQPRDNGNKSSKTDDLGSLRLNICYTEDYVLPSEYYGPLKTLLLKSPDVQPISASAAYILSEICRDKNDAVLPLVRLLLHHDKLVPFATAVAELDLKDTQDANTIFRGNSLATRCLDEMMKIVGGHYLKVTLKPILDEICDSSKSCEIDPIKLKEGDNVENNKENLRYYVDKLFNTIVKSSMSCPTVMCDIFYSLRQMATQRFPNDPHVQYSAVSSFVFLRFFAVAVVSPHTFHLRPHHPDAQTIRTLTLISKTIQTLGSWGSLSKSKSSFKETFMCEFFKMFQEEGYIIAVKKFLDEISSTETKESSGTSEPVHLKEGEMYKRAQGRTRIGKKNFKKRWFCLTSRELTYHKQPGSKDAIYTIPVKNILAVEKLEESSFNKKNMFQVIHTEKPLYVQANNCVEANEWIDVLCRVSRCNQNRLSFYHPSVYLNGNWLCCQETGENTLGCKPCTAGVPADIQIDIDEDRETERIYSLFTLSLLKLQKMEEACGTIAVYQGPQKEPDDYSNFVIEDSVTTFKTIQQIKSIIEKLDEPHEKYRKKRSSSAKYGSKENPIVGKAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15283 As Substrate

Q15283

Site	PTM Type	Source
A2	Acetylation	Uniprot
K48	Sumoylation	Uniprot
K124	Acetylation	Uniprot
K134	Ubiquitination	Uniprot
K208	Acetylation	Uniprot
K209	Acetylation	Uniprot
K211	Acetylation	Uniprot
K213	Acetylation	Uniprot
K214	Acetylation	Uniprot
S217	Phosphorylation	Uniprot
Y226	Phosphorylation	Uniprot
K302	Ubiquitination	Uniprot
K357	Ubiquitination	Uniprot
K419	Ubiquitination	Uniprot
K423	Ubiquitination	Uniprot
K442	Ubiquitination	Uniprot
K462	Ubiquitination	Uniprot
T537	Phosphorylation	Uniprot
T544	Phosphorylation	Uniprot
S555	Phosphorylation	Uniprot
S557	Phosphorylation	Uniprot
S560	Phosphorylation	Uniprot
K584	Ubiquitination	Uniprot
K607	Ubiquitination	Uniprot
Y612	Phosphorylation	Uniprot
Y651	Phosphorylation	Uniprot
K656	Ubiquitination	Uniprot
T759	Phosphorylation	Uniprot
T782	Phosphorylation	Uniprot
Y786	Phosphorylation	Uniprot
Y796	Phosphorylation	Uniprot
K809	Ubiquitination	Uniprot
K815	Ubiquitination	Uniprot

Research Backgrounds

Q15283_RASA2_HUMAN

Function:

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4).

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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For Research Use Only.
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RASA2 Antibody - #DF2647