PIK3C2B Antibody - #DF2624

(1)  
Product: PIK3C2B Antibody
Catalog: DF2624
Description: Rabbit polyclonal antibody to PIK3C2B
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 185 kDa; 185kD(Calculated).
Uniprot: O00750
RRID: AB_2839830

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Horse(82%), Sheep(80%), Rabbit(91%), Dog(82%)
Clonality:
Polyclonal
Specificity:
PIK3C2B Antibody detects endogenous levels of total PIK3C2B.
RRID:
AB_2839830
Cite Format: Affinity Biosciences Cat# DF2624, RRID:AB_2839830.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C2 PI3K; phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide; phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta; phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta; Phosphoinositide 3 Kinase C2 beta; Phosphoinositide 3-Kinase class 2, beta; phosphoinositide-3-kinase, class 2, beta polypeptide; PI3K C2beta; PtdIns 3 kinase C2 beta; PTDINS-3-kinase C2 beta; ptdIns-3-kinase C2 subunit beta;

Immunogens

Immunogen:
Uniprot:

O00750(P3C2B_HUMAN) >>Visit HPA database.

Gene(ID):
PIK3C2B(5287)
Expression:
O00750 P3C2B_HUMAN:

Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney.

Sequence:
MSSTQGNGEHWKSLESVGISRKELAMAEALQMEYDALSRLRHDKEENRAKQNADPSLISWDEPGVDFYSKPAGRRTDLKLLRGLSGSDPTLNYNSLSPQEGPPNHSTSQGPQPGSDPWPKGSLSGDYLYIFDGSDGGVSSSPGPGDIEGSCKKLSPPPLPPRASIWDTPPLPPRKGSPSSSKISQPSDINTFSLVEQLPGKLLEHRILEEEEVLGGGGQGRLLGSVDYDGINDAITRLNLKSTYDAEMLRDATRGWKEGRGPLDFSKDTSGKPVARSKTMPPQVPPRTYASRYGNRKNATPGKNRRISAAPVGSRPHTVANGHELFEVSEERDEEVAAFCHMLDILRSGSDIQDYFLTGYVWSAVTPSPEHLGDEVNLKVTVLCDRLQEALTFTCNCSSTVDLLIYQTLCYTHDDLRNVDVGDFVLKPCGLEEFLQNKHALGSHEYIQYCRKFDIDIRLQLMEQKVVRSDLARTVNDDQSPSTLNYLVHLQERPVKQTISRQALSLLFDTYHNEVDAFLLADGDFPLKADRVVQSVKAICNALAAVETPEITSALNQLPPCPSRMQPKIQKDPSVLAVRENREKVVEALTAAILDLVELYCNTFNADFQTAVPGSRKHDLVQEACHFARSLAFTVYATHRIPIIWATSYEDFYLSCSLSHGGKELCSPLQTRRAHFSKYLFHLIVWDQQICFPVQVNRLPRETLLCATLYALPIPPPGSSSEANKQRRVPEALGWVTTPLFNFRQVLTCGRKLLGLWPATQENPSARWSAPNFHQPDSVILQIDFPTSAFDIKFTSPPGDKFSPRYEFGSLREEDQRKLKDIMQKESLYWLTDADKKRLWEKRYYCHSEVSSLPLVLASAPSWEWACLPDIYVLLKQWTHMNHQDALGLLHATFPDQEVRRMAVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAVSDLRVTHYFFWLLKDGLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNALAKLAQQVREAAPSARQGILRTGLEEVKQFFALNGSCRLPLSPSLLVKGIVPRDCSYFNSNAVPLKLSFQNVDPLGENIRVIFKCGDDLRQDMLTLQMIRIMSKIWVQEGLDMRMVIFRCFSTGRGRGMVEMIPNAETLRKIQVEHGVTGSFKDRPLADWLQKHNPGEDEYEKAVENFIYSCAGCCVATYVLGICDRHNDNIMLKTTGHMFHIDFGRFLGHAQMFGNIKRDRAPFVFTSDMAYVINGGDKPSSRFHDFVDLCCQAYNLIRKHTHLFLNLLGLMLSCGIPELSDLEDLKYVYDALRPQDTEANATTYFTRLIESSLGSVATKLNFFIHNLAQMKFTGSDDRLTLSFASRTHTLKSSGRISDVFLCRHEKIFHPNKGYIYVVKVMRENTHEATYIQRTFEEFQELHNKLRLLFPSSHLPSFPSRFVIGRSRGEAVAERRREELNGYIWHLIHAPPEVAECDLVYTFFHPLPRDEKAMGTSPAPKSSDGTWARPVGKVGGEVKLSISYKNNKLFIMVMHIRGLQLLQDGNDPDPYVKIYLLPDPQKTTKRKTKVARKTCNPTYNEMLVYDGIPKGDLQQRELQLSVLSEQGFWENVLLGEVNIRLRELDLAQEKTGWFALGSRSHGTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Pig
82
Horse
82
Dog
82
Sheep
80
Bovine
73
Chicken
64
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00750 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
K12 Ubiquitination
S13 Phosphorylation
K22 Ubiquitination
Y34 Phosphorylation
K44 Ubiquitination
K50 Ubiquitination
Y68 Phosphorylation
S69 Phosphorylation
K70 Ubiquitination
K79 Ubiquitination
S115 Phosphorylation
Y127 Phosphorylation
S155 Phosphorylation
T168 Phosphorylation
S177 Phosphorylation
S179 Phosphorylation
K182 Ubiquitination
K201 Ubiquitination
S225 Phosphorylation
Y228 Phosphorylation
K241 Ubiquitination
K267 Ubiquitination
Y293 Phosphorylation
S308 Phosphorylation
K452 Ubiquitination
K465 Ubiquitination
S469 Phosphorylation
K496 Ubiquitination
K571 Ubiquitination
K617 Ubiquitination
T738 Phosphorylation
K752 Ubiquitination
K801 Ubiquitination
K818 Ubiquitination
K825 Ubiquitination
K836 Ubiquitination
K1027 Ubiquitination
K1047 Ubiquitination
K1065 Ubiquitination
K1140 Ubiquitination
T1148 Phosphorylation
S1150 Phosphorylation
K1152 Ubiquitination
K1162 Ubiquitination
K1228 Ubiquitination
K1377 Ubiquitination
K1383 Ubiquitination
Y1401 Phosphorylation
K1415 Ubiquitination
K1482 Ubiquitination
T1486 Phosphorylation
K1491 Ubiquitination
K1503 Ubiquitination
K1515 Ubiquitination
Y1541 Phosphorylation
K1552 Ubiquitination
K1563 Ubiquitination

Research Backgrounds

Function:

Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades.

Subcellular Location:

Microsome. Cell membrane. Cytoplasm>Cytosol. Nucleus. Endoplasmic reticulum.
Note: Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney.

Subunit Structure:

Part of a complex with ERBB2 and EGFR. Part of a complex with phosphorylated EGFR and GRB2. Interacts with phosphorylated EGFR and PDGFR, maybe indirectly. Interacts with GRB2 and SHC1.

Family&Domains:

Belongs to the PI3/PI4-kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.