ZBTB16 Antibody | Affinity Biosciences

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FIGURE 13 Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot.

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot.

Product:	ZBTB16 Antibody
Catalog:	DF2611
Description:	Rabbit polyclonal antibody to ZBTB16
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Rabbit, Dog, Chicken
Mol.Wt.:	85 kDa; 74kD(Calculated).
Uniprot:	Q05516
RRID:	AB_2839817

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Rabbit(92%), Dog(92%), Chicken(82%)

Clonality:

Polyclonal

Specificity:

ZBTB16 Antibody detects endogenous levels of total ZBTB16.

RRID:

AB_2839817
Cite Format: Affinity Biosciences Cat# DF2611, RRID:AB_2839817.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AI467657; lu; PLZF; Promyelocytic leukemia zinc finger protein; ZBT16_HUMAN; ZBTB16; Zfp145; Zinc finger and BTB domain containing 16; zinc finger and BTB domain containing protein 16; Zinc finger and BTB domain-containing protein 16; Zinc finger protein 145 (Kruppel like expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; Znf145;

Immunogens

Immunogen:

Uniprot:

Q05516(ZBT16_HUMAN) >>Visit HPA database.

Gene(ID):

ZBTB16(7704)

Expression:

Q05516 ZBT16_HUMAN:

Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.

Description:

Probable transcription factor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Sequence:

Q05516 ZBT16_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGEKPFECKLCHQRSRDYSAMIKHLRTHNGASPYQCTICTEYCPSLSSMQKHMKGHKPEEIPPDWRIEKTYLYLCYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

Rabbit

Chicken

Horse

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05516 As Substrate

Q05516

Site	PTM Type	Enzyme	Source
K154	Ubiquitination		Uniprot
S184	Phosphorylation		Uniprot
S197	Phosphorylation	P24941 (CDK2)	Uniprot
K242	Sumoylation		Uniprot
T282	Phosphorylation	P24941 (CDK2)	Uniprot
Y334	Phosphorylation		Uniprot
K387	Methylation		Uniprot
K387	Sumoylation		Uniprot
K396	Methylation		Uniprot
K396	Sumoylation		Uniprot
T569	Phosphorylation		Uniprot
T597	Phosphorylation		Uniprot
Y630	Phosphorylation		Uniprot
K647	Acetylation		Uniprot
K650	Acetylation		Uniprot
K653	Acetylation		Uniprot

Research Backgrounds

Q05516_ZBT16_HUMAN

Function:

Acts as a transcriptional repressor. May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Subcellular Location:

Nucleus. Nucleus>Nuclear body.

Tissue Specificity:

Subunit Structure:

Binds EPN1. Interacts with ZBTB32 and CUL3. Interacts with ATP7B. Interacts (via C2H2-type zinc finger domains 1 and 2) with RNF112.

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

References

1). Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction. CELL PROLIFERATION, 2022 (PubMed: 35297519) [IF=8.5]

Application: WB Species: Rat Sample: testicular tissues

FIGURE 13 Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p‐Akt, caspase‐9, Bcl‐2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3. (B) Statistical analysis of band intensity by Student's t‐test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. NS, not significant; Sham, sham group; Surgical treatment, surgical treatment group; VC, varicocele group

Application: WB Species: rat Sample: Testicular

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p-Akt, caspase-9, Bcl-2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3.

2). JAK1, SKI, ZBTB16 as potential biomarkers mediate the inflammatory response in keratoconjunctivitis sicca. Gene, 2024 (PubMed: 38876403) [IF=3.5]

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ZBTB16 Antibody - #DF2611