PAX4 Antibody | Affinity Biosciences

IF/ICC
Cites

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Product:	PAX4 Antibody
Catalog:	DF2605
Description:	Rabbit polyclonal antibody to PAX4
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	42 kDa; 38kD(Calculated).
Uniprot:	O43316
RRID:	AB_2839811

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(92%), Sheep(83%), Rabbit(92%)

Clonality:

Polyclonal

Specificity:

PAX4 Antibody detects endogenous levels of total PAX4.

RRID:

AB_2839811
Cite Format: Affinity Biosciences Cat# DF2605, RRID:AB_2839811.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KPD; MGC129960; MODY9; Paired box 4; Paired box gene 4; paired box homeotic gene 4; Paired box protein Pax-4; Paired domain gene 4; Pax4; PAX4_HUMAN;

Immunogens

Immunogen:

Uniprot:

O43316(PAX4_HUMAN) >>Visit HPA database.

Gene(ID):

PAX4(5078)

Description:

Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.

Sequence:

O43316 PAX4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MHQDGISSMNQLGGLFVNGRPLPLDTRQQIVRLAVSGMRPCDISRILKVSNGCVSKILGRYYRTGVLEPKGIGGSKPRLATPPVVARIAQLKGECPALFAWEIQRQLCAEGLCTQDKTPSVSSINRVLRALQEDQGLPCTRLRSPAVLAPAVLTPHSGSETPRGTHPGTGHRNRTIFSPSQAEALEKEFQRGQYPDSVARGKLATATSLPEDTVRVWFSNRRAKWRRQEKLKWEMQLPGASQGLTVPRVAPGIISAQQSPGSVPTAALPALEPLGPSCYQLCWATAPERCLSDTPPKACLKPCWDCGSFLLPVIAPSCVDVAWPCLDASLAHHLIGGAGKATPTHFSHWP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Rabbit

Sheep

Zebrafish

Dog

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43316 As Substrate

O43316

Site	PTM Type	Enzyme	Source
T26	Phosphorylation		Uniprot
T64	Phosphorylation		Uniprot

Research Backgrounds

O43316_PAX4_HUMAN

Function:

Subcellular Location:

Nucleus.

Family&Domains:

Belongs to the paired homeobox family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

References

1). In utero exposure to phenanthrene induced islet cell dysfunction in adult mice: sex differences in the effects and potential causes. Science of The Total Environment (PubMed: 33513515) [IF=9.8]

Application: WB Species: mice Sample: islet cell

Fig. 4. The expression of pancreatic proteins that participate in islet cell function of adult F1 mice. The data were shown as the mean ± SE and analyzed with SPSS 19.0 software. Significant difference (p < 0.05) was tested using one-way ANOVA (Duncan's test), which are labeled by *. n = 4, 3 dams from each group, and 1–2 F1 mice from each dam.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PAX4 Antibody - #DF2605