ORC5 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Mouse spleen, using ORC5 Antibody.

Product:	ORC5 Antibody
Catalog:	DF2603
Description:	Rabbit polyclonal antibody to ORC5
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	50 kDa; 50kD(Calculated).
Uniprot:	O43913
RRID:	AB_2839809

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%)

Clonality:

Polyclonal

Specificity:

ORC5 Antibody detects endogenous levels of total ORC5.

RRID:

AB_2839809
Cite Format: Affinity Biosciences Cat# DF2603, RRID:AB_2839809.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ORC5; ORC5_HUMAN; ORC5L; ORC5P; ORC5T; Origin recognition complex subunit 5; Origin recognition complex subunit 5 like;

Immunogens

Immunogen:

Uniprot:

O43913(ORC5_HUMAN) >>Visit HPA database.

Gene(ID):

ORC5(5001)

Expression:

O43913 ORC5_HUMAN:

Abundant in spleen, ovary, prostate, testis, and colon mucosa.

Description:

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

Sequence:

O43913 ORC5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPHLENVVLCRESQVSILQSLFGERHHFSFPSIFIYGHTASGKTYVTQTLLKTLELPHVFVNCVECFTLRLLLEQILNKLNHLSSSEDGCSTEITCETFNDFVRLFKQVTTAENLKDQTVYIVLDKAEYLRDMEANLLPGFLRLQELADRNVTVLFLSEIVWEKFRPNTGCFEPFVLYFPDYSIGNLQKILSHDHPPEYSADFYAAYINILLGVFYTVCRDLKELRHLAVLNFPKYCEPVVKGEASERDTRKLWRNIEPHLKKAMQTVYLREISSSQWEKLQKDDTDPGQLKGLSAHTHVELPYYSKFILIAAYLASYNPARTDKRFFLKHHGKIKKTNFLKKHEKTSNHLLGPKPFPLDRLLAILYSIVDSRVAPTANIFSQITSLVTLQLLTLVGHDDQLDGPKYKCTVSLDFIRAIARTVNFDIIKYLYDFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43913 As Substrate

O43913

Site	PTM Type	Source
S86	Phosphorylation	Uniprot
K107	Ubiquitination	Uniprot
K116	Ubiquitination	Uniprot
K126	Ubiquitination	Uniprot
K235	Ubiquitination	Uniprot
Y236	Phosphorylation	Uniprot
K242	Ubiquitination	Uniprot
K263	Ubiquitination	Uniprot
S274	Phosphorylation	Uniprot
K280	Ubiquitination	Uniprot
K283	Ubiquitination	Uniprot
K292	Ubiquitination	Uniprot
K330	Acetylation	Uniprot

Research Backgrounds

O43913_ORC5_HUMAN

Function:

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

PTMs:

Multi-mono-ubiquitinated by OBI1; ubiquitination is important for efficient DNA replication origin site activation. Ubiquitination levels are low in mitotic and early G1-phAse cells and are induced in late G1-/early S-phase, peaking in S-phase and decrease toward the end of the cell cycle.

Subcellular Location:

Nucleus. Chlorosome.

Tissue Specificity:

Abundant in spleen, ovary, prostate, testis, and colon mucosa.

Subunit Structure:

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.

Family&Domains:

Belongs to the ORC5 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

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ORC5 Antibody - #DF2603