ZDHHC13 Antibody - #DF2600

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Product: ZDHHC13 Antibody
Catalog: DF2600
Description: Rabbit polyclonal antibody to ZDHHC13
Application: WB IHC
Reactivity: Human, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 58 kDa; 71kD(Calculated).
Uniprot: Q8IUH4
RRID: AB_2839806

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(90%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
ZDHHC13 Antibody detects endogenous levels of total ZDHHC13.
RRID:
AB_2839806
Cite Format: Affinity Biosciences Cat# DF2600, RRID:AB_2839806.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHHC 13; DHHC-13; FLJ10852; FLJ10941; HIP14 like; HIP14 related protein; HIP14-related protein; HIP14L; HIP3RP; Huntingtin interacting protein 14 related protein; Huntingtin interacting protein HIP3RP; Huntingtin-interacting protein 14-related protein; Huntingtin-interacting protein HIP3RP; Hypothetical protein similar to ankyrin repeat containing priotein AKR1; MGC64994; Palmitoyltransferase ZDHHC13; Probable palmitoyltransferase ZDHHC13; Putative MAPK activating protein PM03; Putative MAPK-activating protein PM03; Putative NF kappa B activating protein 209; Putative NF-kappa-B-activating protein 209; ZDH13_HUMAN; ZDHHC 13; ZDHHC13; Zinc finger DHHC domain containing 13; Zinc finger DHHC domain-containing protein 13;

Immunogens

Immunogen:
Uniprot:

Q8IUH4(ZDH13_HUMAN) >>Visit HPA database.

Gene(ID):
ZDHHC13(54503)
Description:
Palmitoyltransferase for HD and GAD2 (By similarity). Mediates Mg(2+) transport.
Sequence:
MEGPGLGSQCRNHSHGPHPPGFGRYGICAHENKELANAREALPLIEDSSNCDIVKATQYGIFERCKELVEAGYDVRQPDKENVSLLHWAAINNRLDLVKFYISKGAVVDQLGGDLNSTPLHWAIRQGHLPMVILLLQHGADPTLIDGEGFSSIHLAVLFQHMPIIAYLISKGQSVNMTDVNGQTPLMLSAHKVIGPEPTGFLLKFNPSLNVVDKIHQNTPLHWAVAAGNVNAVDKLLEAGSSLDIQNVKGETPLDMALQNKNQLIIHMLKTEAKMRANQKFRLWRWLQKCELFLLLMLSVITMWAIGYILDFNSDSWLLKGCLLVTLFFLTSLFPRFLVGYKNLVYLPTAFLLSSVFWIFMTWFILFFPDLAGAPFYFSFIFSIVAFLYFFYKTWATDPGFTKASEEEKKVNIITLAETGSLDFRTFCTSCLIRKPLRSLHCHVCNCCVARYDQHCLWTGRCIGFGNHHYYIFFLFFLSMVCGWIIYGSFIYLSSHCATTFKEDGLWTYLNQIVACSPWVLYILMLATFHFSWSTFLLLNQLFQIAFLGLTSHERISLQKQSKHMKQTLSLRKTPYNLGFMQNLADFFQCGCFGLVKPCVVDWTSQYTMVFHPAREKVLRSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Rabbit
90
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IUH4 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S8 Phosphorylation
Y25 Phosphorylation
K33 Ubiquitination
K55 Ubiquitination
Y59 Phosphorylation
K66 Ubiquitination
Y73 Phosphorylation
R76 Methylation
K80 Ubiquitination
K192 Ubiquitination
K214 Ubiquitination
K235 Ubiquitination
K249 Ubiquitination
K261 Ubiquitination
K566 Ubiquitination

Research Backgrounds

Function:

Palmitoyltransferase for HTT and GAD2. May play a role in Mg(2+) transport.

Subcellular Location:

Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane>Multi-pass membrane protein.
Note: Low extracellular Mg(2+) induces increase in Golgi and in post-Golgi vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via ANK repeats) with CLIP3. Interacts (via ANK repeats) with DNAJC5 (via C-terminus) (By similarity). Interacts (via ANK repeats) with HTT. Interacts (via ANK repeats) with MAP6. Interacts (via ANK repeats) with SNAP23. Interacts (via ANK repeats) with SNAP25. May interact (via ANK repeats) with SPRED2 (By similarity).

Family&Domains:

The DHHC domain is required for palmitoyltransferase activity.

Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.

References

1). A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chemical Biology, 2021 (PubMed: 34458782) [IF=4.1]

Application: WB    Species: Human    Sample: A375 cells

Fig. 3 PD-1 is palmitoylated by DHHC9. (A) Western blot showing the effect of two independent siRNAs targeting the indicated DHHC enzymes on DHHC protein levels in A375 cells with the specific antibody for each DHHC enzyme. (B) Western blot showing the expression of PD-1 in A375 cells treated by two independent siRNAs targeting the indicated DHHC enzymes. (C) Western blot showing the expression of PD-1 in RKO and HCT116 cells treated by two independent siRNAs targeting DHHC9. Statistics shown on the right, *** P o 0.001, ** P o 0.01, ANOVA test. n = 3 independent experiments. (D) Immunoblot showing the expression of PD-1 in different cells overexpressing DHHC9. Statistics shown on the right, *** P o 0.001, ** P o 0.01, ANOVA test. n = 3 independent experiments.

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