SMARCAD1 Antibody - #DF2598

(1)  
Product: SMARCAD1 Antibody
Catalog: DF2598
Description: Rabbit polyclonal antibody to SMARCAD1
Application: WB IHC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 117 kDa; 117kD(Calculated).
Uniprot: Q9H4L7
RRID: AB_2839804

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
SMARCAD1 Antibody detects endogenous levels of total SMARCAD1.
RRID:
AB_2839804
Cite Format: Affinity Biosciences Cat# DF2598, RRID:AB_2839804.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent helicase 1; ATP-dependent helicase 1; ETL1; hHEL1; Smarcad1; SMRCD_HUMAN; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1;

Immunogens

Immunogen:
Uniprot:

Q9H4L7(SMRCD_HUMAN) >>Visit HPA database.

Gene(ID):
SMARCAD1(56916)
Expression:
Q9H4L7 SMRCD_HUMAN:

Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level) (PubMed:29409814).

Description:
Probable ATP-dependent DNA helicase.
Sequence:
MNLFNLDRFRFEKRNKIEEAPEATPQPSQPGPSSPISLSAEEENAEGEVSRANTPDSDITEKTEDSSVPETPDNERKASISYFKNQRGIQYIDLSSDSEDVVSPNCSNTVQEKTFNKDTVIIVSEPSEDEESQGLPTMARRNDDISELEDLSELEDLKDAKLQTLKELFPQRSDNDLLKLIESTSTMDGAIAAALLMFGDAGGGPRKRKLSSSSEPYEEDEFNDDQSIKKTRLDHGEESNESAESSSNWEKQESIVLKLQKEFPNFDKQELREVLKEHEWMYTEALESLKVFAEDQDMQYVSQSEVPNGKEVSSRSQNYPKNATKTKLKQKFSMKAQNGFNKKRKKNVFNPKRVVEDSEYDSGSDVGSSLDEDYSSGEEVMEDGYKGKILHFLQDASIGELTLIPQCSQKKAQKITELRPFNSWEALFTKMSKTNGLSEDLIWHCKTLIQERDVVIRLMNKCEDISNKLTKQVTMLTGNGGGWNIEQPSILNQSLSLKPYQKVGLNWLALVHKHGLNGILADEMGLGKTIQAIAFLAYLYQEGNNGPHLIVVPASTIDNWLREVNLWCPTLKVLCYYGSQEERKQIRFNIHSRYEDYNVIVTTYNCAISSSDDRSLFRRLKLNYAIFDEGHMLKNMGSIRYQHLMTINANNRLLLTGTPVQNNLLELMSLLNFVMPHMFSSSTSEIRRMFSSKTKSADEQSIYEKERIAHAKQIIKPFILRRVKEEVLKQLPPKKDRIELCAMSEKQEQLYLGLFNRLKKSINNLEKNTEMCNVMMQLRKMANHPLLHRQYYTAEKLKEMSQLMLKEPTHCEANPDLIFEDMEVMTDFELHVLCKQYRHINNFQLDMDLILDSGKFRVLGCILSELKQKGDRVVLFSQFTMMLDILEVLLKHHQHRYLRLDGKTQISERIHLIDEFNTDMDIFVFLLSTKAGGLGINLTSANVVILHDIDCNPYNDKQAEDRCHRVGQTKEVLVIKLISQGTIEESMLKINQQKLKLEQDMTTVDEGDEGSMPADIATLLKTSMGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H4L7 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S33 Phosphorylation
S34 Phosphorylation
S37 Phosphorylation
S39 Phosphorylation
S50 Phosphorylation
T54 Phosphorylation
S57 Phosphorylation
T60 Phosphorylation
T63 Phosphorylation
S66 Phosphorylation
S67 Phosphorylation
T71 Phosphorylation
K77 Sumoylation
K77 Ubiquitination
S79 Phosphorylation
Y82 Phosphorylation
K84 Sumoylation
S95 Phosphorylation
S96 Phosphorylation
S98 Phosphorylation
S103 Phosphorylation
T114 Phosphorylation
T119 Phosphorylation
S124 Phosphorylation
S127 Phosphorylation
S132 Phosphorylation
T137 Phosphorylation
S146 Phosphorylation
S152 Phosphorylation
K158 Ubiquitination
K161 Sumoylation
K161 Ubiquitination
S173 Phosphorylation
K209 Ubiquitination
S211 Phosphorylation
S212 Phosphorylation
S213 Phosphorylation
S214 Phosphorylation
Y217 Phosphorylation
S239 Phosphorylation
S242 Phosphorylation
S245 Phosphorylation
S254 Phosphorylation
K258 Sumoylation
K258 Ubiquitination
K261 Ubiquitination
K268 Methylation
K268 Ubiquitination
K276 Ubiquitination
S302 Phosphorylation
S304 Phosphorylation
K310 Ubiquitination
S313 Phosphorylation
S314 Phosphorylation
S316 Phosphorylation
Y319 Phosphorylation
K335 Sumoylation
K346 Sumoylation
K346 Ubiquitination
K352 Acetylation
K388 Ubiquitination
S397 Phosphorylation
K410 Ubiquitination
S423 Phosphorylation
K430 Ubiquitination
K446 Ubiquitination
K461 Ubiquitination
K468 Ubiquitination
K695 Sumoylation
K695 Ubiquitination
K705 Ubiquitination
K712 Ubiquitination
K716 Sumoylation
K724 Sumoylation
K724 Ubiquitination
K729 Acetylation
K760 Ubiquitination
K767 Ubiquitination
K780 Ubiquitination
K796 Ubiquitination
K798 Ubiquitination
S801 Phosphorylation
S853 Phosphorylation
T880 Phosphorylation
Y897 Phosphorylation
K903 Ubiquitination
K970 Ubiquitination
K989 Ubiquitination
K994 Acetylation

Research Backgrounds

Function:

DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing.

Subcellular Location:

Nucleus. Chromosome.
Note: Colocalizes with PCNA at replication forks during S phase. Recruited to double-strand breaks (DSBs) sites of DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is expressed ubiquitously. Isoform 3 is expressed mainly in skin and esophagus. Expressed in fibroblasts and keratinocytes (at protein level).

Subunit Structure:

Binds to DNA preferentially in the vicinity of transcriptional start sites. Interacts with MSH2 and TRIM28. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with PCNA.

Family&Domains:

Belongs to the SNF2/RAD54 helicase family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.