CANT1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat brain, using CANT1 Antibody.

Product:	CANT1 Antibody
Catalog:	DF2590
Description:	Rabbit polyclonal antibody to CANT1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	45 kDa; 45kD(Calculated).
Uniprot:	Q8WVQ1
RRID:	AB_2839796

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(90%), Horse(90%), Rabbit(90%), Dog(90%), Chicken(100%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

CANT1 Antibody detects endogenous levels of total CANT1.

RRID:

AB_2839796
Cite Format: Affinity Biosciences Cat# DF2590, RRID:AB_2839796.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Apyrase; Apyrase homolog; Ca2+ dependent endoplasmic reticulum nucleoside diphosphatase; Calcium activated nucleotidase 1; CANT 1; CANT1; CANT1_HUMAN; Putative MAPK activating protein PM09; Putative MAPK-activating protein PM09; Putative NF kappa B activating protein 107; Putative NF-kappa-B-activating protein 107; SCAN 1; SCAN-1; SHAPY; Soluble Ca activated nucleotidase isozyme 1; Soluble calcium activated nucleotidase 1; Soluble calcium activated nucleotidase SCAN 1; Soluble calcium-activated nucleotidase 1;

Immunogens

Immunogen:

Uniprot:

Q8WVQ1(CANT1_HUMAN) >>Visit HPA database.

Gene(ID):

CANT1(124583)

Expression:

Q8WVQ1 CANT1_HUMAN:

Widely expressed.

Description:

Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP.

Sequence:

Q8WVQ1 CANT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPVQLSEHPEWNESMHSLRISVGGLPVLASMTKAADPRFRPRWKVILTFFVGAAILWLLCSHRPAPGRPPTHNAHNWRLGQAPANWYNDTYPLSPPQRTPAGIRYRIAVIADLDTESRAQEENTWFSYLKKGYLTLSDSGDKVAVEWDKDHGVLESHLAEKGRGMELSDLIVFNGKLYSVDDRTGVVYQIEGSKAVPWVILSDGDGTVEKGFKAEWLAVKDERLYVGGLGKEWTTTTGDVVNENPEWVKVVGYKGSVDHENWVSNYNALRAAAGIQPPGYLIHESACWSDTLQRWFFLPRRASQERYSEKDDERKGANLLLSASPDFGDIAVSHVGAVVPTHGFSSFKFIPNTDDQIIVALKSEEDSGRVASYIMAFTLDGRFLLPETKIGSVKYEGIEFI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Chicken

100

Pig

Horse

Bovine

Dog

Xenopus

Rabbit

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WVQ1 As Substrate

Q8WVQ1

Site	PTM Type	Source
S17	Phosphorylation	Uniprot
S21	Phosphorylation	Uniprot
Y133	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
S139	Phosphorylation	Uniprot
S156	Phosphorylation	Uniprot
K161	Ubiquitination	Uniprot
S202	Phosphorylation	Uniprot
S303	Phosphorylation	Uniprot

Research Backgrounds

Q8WVQ1_CANT1_HUMAN

Function:

PTMs:

N-glycosylated.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein. Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein. Cell membrane.
Note: Processed form: Secreted.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Monomer. Homodimer; dimerization is Ca(2+)-dependent. Homodimer; disulfide-linked (membrane form).

Family&Domains:

Belongs to the apyrase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CANT1 Antibody - #DF2590