REV1 Antibody - #DF2581

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Product: REV1 Antibody
Catalog: DF2581
Description: Rabbit polyclonal antibody to REV1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 138 kDa; 138kD(Calculated).
Uniprot: Q9UBZ9
RRID: AB_2839787

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
REV1 Antibody detects endogenous levels of total REV1.
RRID:
AB_2839787
Cite Format: Affinity Biosciences Cat# DF2581, RRID:AB_2839787.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AIBP 80; AIBP80; Alpha integrin binding protein 80; Alpha integrin-binding protein 80; DNA repair protein REV 1; DNA repair protein REV1; FLJ21523; MGC163283; MGC26225; REV 1 (yeast homolog) like; REV 1; REV 1 homolog; REV 1 like; REV 1 protein; REV 1L; REV1 (yeast homolog) like; REV1; REV1 homolog (S. cerevisiae); REV1 homolog; REV1 like (yeast); REV1 like; Rev1 like terminal deoxycytidyl transferase; REV1 protein; Rev1-like terminal deoxycytidyl transferase; REV1_HUMAN; REV1L;

Immunogens

Immunogen:
Uniprot:

Q9UBZ9(REV1_HUMAN) >>Visit HPA database.

Gene(ID):
REV1(51455)
Expression:
Q9UBZ9 REV1_HUMAN:

Ubiquitous.

Description:
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
Sequence:
MRRGGWRKRAENDGWETWGGYMAAKVQKLEEQFRSDAAMQKDGTSSTIFSGVAIYVNGYTDPSAEELRKLMMLHGGQYHVYYSRSKTTHIIATNLPNAKIKELKGEKVIRPEWIVESIKAGRLLSYIPYQLYTKQSSVQKGLSFNPVCRPEDPLPGPSNIAKQLNNRVNHIVKKIETENEVKVNGMNSWNEEDENNDFSFVDLEQTSPGRKQNGIPHPRGSTAIFNGHTPSSNGALKTQDCLVPMVNSVASRLSPAFSQEEDKAEKSSTDFRDCTLQQLQQSTRNTDALRNPHRTNSFSLSPLHSNTKINGAHHSTVQGPSSTKSTSSVSTFSKAAPSVPSKPSDCNFISNFYSHSRLHHISMWKCELTEFVNTLQRQSNGIFPGREKLKKMKTGRSALVVTDTGDMSVLNSPRHQSCIMHVDMDCFFVSVGIRNRPDLKGKPVAVTSNRGTGRAPLRPGANPQLEWQYYQNKILKGKAADIPDSSLWENPDSAQANGIDSVLSRAEIASCSYEARQLGIKNGMFFGHAKQLCPNLQAVPYDFHAYKEVAQTLYETLASYTHNIEAVSCDEALVDITEILAETKLTPDEFANAVRMEIKDQTKCAASVGIGSNILLARMATRKAKPDGQYHLKPEEVDDFIRGQLVTNLPGVGHSMESKLASLGIKTCGDLQYMTMAKLQKEFGPKTGQMLYRFCRGLDDRPVRTEKERKSVSAEINYGIRFTQPKEAEAFLLSLSEEIQRRLEATGMKGKRLTLKIMVRKPGAPVETAKFGGHGICDNIARTVTLDQATDNAKIIGKAMLNMFHTMKLNISDMRGVGIHVNQLVPTNLNPSTCPSRPSVQSSHFPSGSYSVRDVFQVQKAKKSTEEEHKEVFRAAVDLEISSASRTCTFLPPFPAHLPTSPDTNKAESSGKWNGLHTPVSVQSRLNLSIEVPSPSQLDQSVLEALPPDLREQVEQVCAVQQAESHGDKKKEPVNGCNTGILPQPVGTVLLQIPEPQESNSDAGINLIALPAFSQVDPEVFAALPAELQRELKAAYDQRQRQGENSTHQQSASASVPKNPLLHLKAAVKEKKRNKKKKTIGSPKRIQSPLNNKLLNSPAKTLPGACGSPQKLIDGFLKHEGPPAEKPLEELSASTSGVPGLSSLQSDPAGCVRPPAPNLAGAVEFNDVKTLLREWITTISDPMEEDILQVVKYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFILDNVQVVLQQTYGSTLKVT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
90
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBZ9 As Substrate

Site PTM Type Enzyme
K28 Ubiquitination
K99 Sumoylation
K107 Ubiquitination
K119 Ubiquitination
K134 Ubiquitination
K140 Ubiquitination
S254 Phosphorylation
S297 Phosphorylation
S301 Phosphorylation
K442 Ubiquitination
K473 Ubiquitination
Y541 Phosphorylation
Y546 Phosphorylation
S607 Phosphorylation
K625 Ubiquitination
T675 Phosphorylation
T687 Phosphorylation
K770 Ubiquitination
S812 Phosphorylation
S901 Phosphorylation
K1033 Ubiquitination
S1082 Phosphorylation
S1088 Phosphorylation
S1097 Phosphorylation
T1101 Phosphorylation
S1108 Phosphorylation

Research Backgrounds

Function:

Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Monomer. Interacts with the DNA polymerase zeta which is composed of REV3L and MAD2L2; the interaction with MAD2L2 is direct and requires that REV3L is in its closed conformation. Interacts with POLH, POLI and POLK. May bind ITGA3. Interacts with FAAP20/C1orf86.

Family&Domains:

The C-terminal domain is necessary for protein interactions.

Belongs to the DNA polymerase type-Y family.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

References

1). Elevated expression of REV1 is a predictor of unfavorable prognosis in patients with prostate cancer. , 2021

Application: WB    Species: Human    Sample: Prostate cancer tissues

Figure 1. Immunostaining of REV1 protein in prostate cancer, normal prostate, and hyperplasia tissues. (A) Immunostaining of TMA from 80 samples. (B) IRSs of REV1 protein in Prostate cancer, normal prostate, and hyperplasia tissues. Expression levels of REV1 protein in Prostate cancer tissues were significantly higher than that in non-cancerous prostate tissues (IRS: Prostate cancer = 4.08±1.30 vs. Normal = 2.50±0.71, P < 0.001; Prostate cancer = 4.08±1.30 vs. Hyperplasia = 3.15±1.14, P = 0.007). **mean P < 0.01; (C, D) Representative immunohistochemical photographs of REV1 protein in Prostate cancer tissues. (E, F) Representative immunohistochemical photographs of REV1 protein in normal and hyperplasia prostate tissues. The red arrows in (C, D) show positive staining cells.

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