SAP130 Antibody - #DF2567

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Product: SAP130 Antibody
Catalog: DF2567
Description: Rabbit polyclonal antibody to SAP130
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 135 kDa; 110kD(Calculated).
Uniprot: Q9H0E3
RRID: AB_2839773

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
SAP130 Antibody detects endogenous levels of total SAP130.
RRID:
AB_2839773
Cite Format: Affinity Biosciences Cat# DF2567, RRID:AB_2839773.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

130 kDa Sin3-associated polypeptide; FLJ12761; Histone deacetylase complex subunit SAP130; KIAA0017; mSin3A associated protein 130; Pre mRNA splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; Sap130; SF3b 130; SF3b130; SF3B3; Sin3-associated polypeptide p130; Sin3A associated protein 130kDa; Sin3A associated protein; SP130_HUMAN; Spliceosome associated protein 130; Splicing factor 3b subunit 3 130kDa; Splicing factor 3b subunit 3; STAF 130; STAF130;

Immunogens

Immunogen:
Uniprot:

Q9H0E3(SP130_HUMAN) >>Visit HPA database.

Gene(ID):
SAP130(79595)
Expression:
Q9H0E3 SP130_HUMAN:

Expressed in various cancer cell ines.

Description:
Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
Sequence:
MGPPRHPQAGEIEAGGAGGGRRLQVEMSSQQFPRLGAPSTGLSQAPSQIANSGSAGLINPAATVNDESGRDSEVSAREHMSSSSSLQSREEKQEPVVVRPYPQVQMLSTHHAVASATPVAVTAPPAHLTPAVPLSFSEGLMKPPPKPTMPSRPIAPAPPSTLSLPPKVPGQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMSIIRSNAPGPPLHIGASHLPRGAAAAAVMSSSKVTTVLRPTSQLPNAATAQPAVQHIIHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSAAISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPILATNTIPSATTAGSVSHTQAPTSTIVTMTVPSHSSHATAVTTSNIPVAKVVPQQITHTSPRIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFLPTYPPSAYPLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQLMTVDASHARHIQGIQPAPISTQGIQPAPIGTPGIQPAPLGTQGIHSATPINTQGLQPAPMGTQQPQPEGKTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQHVISTEEGDMMETNSTDDEKSTAKSLLVKAEKRKSPPKEYIDEEGVRYVPVRPRPPITLLRHYRNPWKAAYHHFQRYSDVRVKEEKKAMLQEIANQKGVSCRAQGWKVHLCAAQLLQLTNLEHDVYERLTNLQEGIIPKKKAATDDDLHRINELIQGNMQRCKLVMDQISEARDSMLKVLDHKDRVLKLLNKNGTVKKVSKLKRKEKV

PTMs - Q9H0E3 As Substrate

Site PTM Type Enzyme
R5 Methylation
R34 Methylation
T63 Phosphorylation
S72 Phosphorylation
R232 Methylation
S300 Phosphorylation
R344 Methylation
S349 Phosphorylation
T355 Phosphorylation
T439 Phosphorylation
T441 Phosphorylation
S442 Phosphorylation
R444 Methylation
R453 Methylation
S454 Phosphorylation
S455 Phosphorylation
S460 Phosphorylation
R463 Methylation
S465 Phosphorylation
T677 Phosphorylation
K783 Sumoylation
K785 Sumoylation
S831 Phosphorylation
T853 Phosphorylation
S855 Phosphorylation
T856 Phosphorylation
K869 Methylation
K869 Sumoylation
S875 Phosphorylation
Y880 Phosphorylation
K937 Ubiquitination
K979 Ubiquitination
K981 Ubiquitination
K1023 Ubiquitination
K1028 Ubiquitination

Research Backgrounds

Function:

Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.

PTMs:

Acetylated.

Sumoylated with SUMO1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in various cancer cell ines.

Subunit Structure:

Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Interacts (released by dead or dying cells) with CLEC4E (By similarity).

Family&Domains:

The N-terminus may interact with a transcriptional coactivator.

The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.

Belongs to the SAP130 family.

Restrictive clause

 

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