PDCD1LG2 Antibody | Affinity Biosciences

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Western blot analysis of PDCD1LG2 expression in ZR-75-1 cell lysates .

Product:	PDCD1LG2 Antibody
Catalog:	DF2561
Description:	Rabbit polyclonal antibody to PDCD1LG2
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Horse, Dog
Mol.Wt.:	31 kDa; 31kD(Calculated).
Uniprot:	Q9BQ51
RRID:	AB_2839767

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(92%), Horse(92%), Dog(83%)

Clonality:

Polyclonal

Specificity:

PDCD1LG2 Antibody detects endogenous levels of total PDCD1LG2.

RRID:

AB_2839767
Cite Format: Affinity Biosciences Cat# DF2561, RRID:AB_2839767.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B7 dendritic cell molecule; B7-DC; B7DC; bA574F11.2; Btdc; Butyrophilin B7 DC; Butyrophilin B7-DC; Butyrophilin B7DC; CD 273; CD273; CD273 antigen; MGC142238; MGC142240; PD 1 ligand 2; PD L2; PD-1 ligand 2; PD-L2; PD1 ligand 2; PD1L2_HUMAN; PDCD 1 ligand 2; PDCD1 ligand 2; PDCD1L2; Pdcd1lg2; PDL 2; PDL2; Programmed cell death 1 ligand 2; Programmed death ligand 2;

Immunogens

Immunogen:

Uniprot:

Q9BQ51(PD1L2_HUMAN) >>Visit HPA database.

Gene(ID):

PDCD1LG2(80380)

Expression:

Q9BQ51 PD1L2_HUMAN:

Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.

Description:

vInvolved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Sequence:

Q9BQ51 PD1L2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIFLLLMLSLELQLHQIAALFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPTWLLHIFIPFCIIAFIFIATVIALRKQLCQKLYSSKDTTKRPVTTTKREVNSAI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BQ51 As Substrate

Q9BQ51

Site	PTM Type	Source
Y114	Phosphorylation	Uniprot
T116	Phosphorylation	Uniprot
T128	Phosphorylation	Uniprot
S159	Phosphorylation	Uniprot
S165	Phosphorylation	Uniprot
S167	Phosphorylation	Uniprot

Research Backgrounds

Q9BQ51_PD1L2_HUMAN

Function:

Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).

Subcellular Location:

Secreted.

Endomembrane system>Single-pass type I membrane protein.

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus.

Subunit Structure:

Interacts with PDCD1.

Family&Domains:

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

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PDCD1LG2 Antibody - #DF2561