Product: KRT81 Antibody
Catalog: DF2559
Description: Rabbit polyclonal antibody to KRT81
Application: WB IHC
Reactivity: Human
Prediction: Horse
Mol.Wt.: 55 kDa.; 55kD(Calculated).
Uniprot: Q14533
RRID: AB_2839765

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(100%)
Clonality:
Polyclonal
Specificity:
KRT81 Antibody detects endogenous levels of total KRT81.
RRID:
AB_2839765
Cite Format: Affinity Biosciences Cat# DF2559, RRID:AB_2839765.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1; basic; ghHb 1; ghHb1; ghHkb 1; ghHKb1; hair; Hair keratin K2.9; Hard keratin type II 1; HB 1; HB1; hHAKB2 1; K2.9; K81; Keratin 81; Keratin; Keratin hair basic 1; Keratin type II cuticular Hb1; Keratin-81; Keratin81; KRT 81; KRT81; KRT81_HUMAN; KRTHB 1; KRTHB1; Metastatic lymph node 137 gene protein; MLN 137; MLN137; type II cuticular Hb1; Type II hair keratin Hb1; Type-II keratin Kb21;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q14533 KRT81_HUMAN:

Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue.

Sequence:
MTCGSGFGGRAFSCISACGPRPGRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFEGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVSLRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEILILQSHISDTSVVVKLDNSRDLNMDCIIAEIKAQYDDIVTRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGIGAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNVAVSTGLCAPCGQLNTTCGGGSCGVGSCGISSLGVGSCGSSCRKC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
78
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14533 As Substrate

Site PTM Type Enzyme
S47 Phosphorylation
S49 Phosphorylation
K132 Ubiquitination
T203 Phosphorylation
S252 Phosphorylation
S258 Phosphorylation
Y282 Phosphorylation
Y297 Phosphorylation
K300 Ubiquitination
K305 Ubiquitination
T307 Phosphorylation
T314 Phosphorylation
T318 Phosphorylation
K319 Ubiquitination
K340 Ubiquitination
K345 Ubiquitination
K367 Ubiquitination
K377 Ubiquitination
K379 Ubiquitination
S395 Phosphorylation

Research Backgrounds

Tissue Specificity:

Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue.

Subunit Structure:

Heterotetramer of two type I and two type II keratins.

Family&Domains:

Belongs to the intermediate filament family.

Restrictive clause

 

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For Research Use Only.
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