KRT85/86 Antibody - #DF2558

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Product: KRT85/86 Antibody
Catalog: DF2558
Description: Rabbit polyclonal antibody to KRT85/86
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 56 kDa,54KDa; 56kD,54kD,55kD,29kD(Calculated).
Uniprot: P78386 | O43790 | P78385 | Q14533 | A6NCN2
RRID: AB_2839764

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KRT85/86 Antibody detects endogenous levels of total KRT85/86.
RRID:
AB_2839764
Cite Format: Affinity Biosciences Cat# DF2558, RRID:AB_2839764.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ECTD4; Hair keratin K2.12; Hard keratin type II 5; Hb 5; HB5; hHb5; K12.12; K85; Keratin 85; keratin hair basic 5; Keratin type II cuticular Hb5; keratin, hard, type II; KRTHB5; type II cuticular Hb5; Type II hair keratin Hb5; Type II keratin Kb25; Hair keratin K2.11; Hb6; K86; Keratin; Keratin-86; KRT86; KRT86_HUMAN; KRTHB6; MNX; type II cuticular Hb6; Type II hair keratin Hb6; Type-II keratin Kb26; Hair keratin K2.10; Hard keratin, type II, 3; HB 3; HB3; hHb3; K2.10; K83; Keratin 83; Keratin; Keratin, hair, basic, 3; Keratin-83; Krt83; KRT83_HUMAN; KRTHB3; type II cuticular Hb3; Type II hair keratin Hb3; Type-II keratin Kb23; 1; basic; ghHb 1; ghHb1; ghHkb 1; ghHKb1; hair; Hair keratin K2.9; Hard keratin type II 1; HB 1; HB1; hHAKB2 1; K2.9; K81; Keratin 81; Keratin; Keratin hair basic 1; Keratin type II cuticular Hb1; Keratin-81; Keratin81; KRT 81; KRT81; KRT81_HUMAN; KRTHB 1; KRTHB1; Metastatic lymph node 137 gene protein; MLN 137; MLN137; type II cuticular Hb1; Type II hair keratin Hb1; Type-II keratin Kb21;

Immunogens

Immunogen:
Uniprot:

P78386(KRT85_HUMAN) >>Visit HPA database.

O43790(KRT86_HUMAN) >>Visit HPA database.

P78385(KRT83_HUMAN) >>Visit HPA database.

Q14533(KRT81_HUMAN) >>Visit HPA database.

A6NCN2(KR87P_HUMAN) >>Visit HPA database.

Gene(ID):
KRT83(3889) | KRT85(3891) | KRT86(3892) | KRT81(3887)
Expression:
P78386 KRT85_HUMAN:

Synthesis occurs immediately above a small population of matrix cells at the base of the hair bulb and the trichocytes lining the dermal papilla and extends upward through the matrix and ends in the lower part of the cortex of the hair shaft.

O43790 KRT86_HUMAN:

Synthesis begins slightly higher in the hair shaft than HB1 and HB3 and continues much farther up, ending in the keratogeneous zone.

P78385 KRT83_HUMAN:

Synthesis begins in the cortex 10-15 cell layers above the apex of the dermal papilla and ends abruptly in the middle of the cortex.

Q14533 KRT81_HUMAN:

Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue.

Sequence:
MSCRSYRISSGCGVTRNFSSCSAVAPKTGNRCCISAAPYRGVSCYRGLTGFGSRSLCNLGSCGPRIAVGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKWQFYQNQRCCESNLEPLFSGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVVLKKDVDCAYLRKSDLEANVEALVEESSFLRRLYEEEIRVLQAHISDTSVIVKMDNSRDLNMDCIIAEIKAQYDDVASRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEIENAKCQRAKLEAAVAEAEQQGEAALSDARCKLAELEGALQKAKQDMACLLKEYQEVMNSKLGLDIEIATYRRLLEGEEHRLCEGVGSVNVCVSSSRGGVSCGGLSYSTTPGRQITSGPSAIGGSITVVAPDSCAPCQPRSSSFSCGSSRSVRFA

MTCGSYCGGRAFSCISACGPRPGRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFEGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVSLRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEIRVLQSHISDTSVVVKLDNSRDLNMDCIIAEIKAQYDDIVTRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGVGSVNVCVSSSRGGVVCGDLCASTTAPVVSTRVSSVPSNSNVVVGTTNACAPSARVGVCGGSCKRC

MTCGFNSIGCGFRPGNFSCVSACGPRPSRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFAGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEIRILQSHISDTSVVVKLDNSRDLNMDCIVAEIKAQYDDIATRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGVEAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNLVVSTGLCKPCGQLNTTCGGGSCGQGRH

MTCGSGFGGRAFSCISACGPRPGRCCITAAPYRGISCYRGLTGGFGSHSVCGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKLQFYQNRECCQSNLEPLFEGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVSLRATAENEFVALKKDVDCAYLRKSDLEANVEALIQEIDFLRRLYEEEILILQSHISDTSVVVKLDNSRDLNMDCIIAEIKAQYDDIVTRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGIGAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNVAVSTGLCAPCGQLNTTCGGGSCGVGSCGISSLGVGSCGSSCRKC

MEANSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVALKKDVDCAYLRKSDLEANVEALTQEIDFLRRLYEEEIRVLQSHISDTSVVVKMDNSRDLNMHCVITEIKAQYDDIATRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLAWTLRSPPTGACWRARSRGCVRALVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78386/O43790/P78385/Q14533/A6NCN2 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K149 Ubiquitination
K229 Sumoylation
K229 Ubiquitination
K230 Ubiquitination
Y260 Phosphorylation
Y314 Phosphorylation
K317 Ubiquitination
K322 Ubiquitination
T324 Phosphorylation
T331 Phosphorylation
T335 Phosphorylation
K336 Ubiquitination
K384 Ubiquitination
K394 Ubiquitination
K396 Ubiquitination
S412 Phosphorylation
S493 Phosphorylation
S494 Phosphorylation
Site PTM Type Enzyme
S47 Phosphorylation
S49 Phosphorylation
K132 Ubiquitination
K193 Methylation
T203 Phosphorylation
K222 Methylation
Y243 Phosphorylation
Y282 Phosphorylation
Y297 Phosphorylation
K300 Ubiquitination
K305 Ubiquitination
T307 Phosphorylation
T314 Phosphorylation
T318 Phosphorylation
K319 Ubiquitination
K340 Ubiquitination
K345 Ubiquitination
K367 Ubiquitination
K377 Methylation
K377 Ubiquitination
K379 Ubiquitination
S395 Phosphorylation
S455 Phosphorylation
S460 Phosphorylation
T466 Phosphorylation
S482 Phosphorylation
Site PTM Type Enzyme
S52 Phosphorylation
S54 Phosphorylation
K137 Ubiquitination
Y248 Phosphorylation
S257 Phosphorylation
S263 Phosphorylation
Y302 Phosphorylation
T312 Phosphorylation
T319 Phosphorylation
T323 Phosphorylation
K372 Ubiquitination
S400 Phosphorylation
Site PTM Type Enzyme
S47 Phosphorylation
S49 Phosphorylation
K132 Ubiquitination
T203 Phosphorylation
S252 Phosphorylation
S258 Phosphorylation
Y282 Phosphorylation
Y297 Phosphorylation
K300 Ubiquitination
K305 Ubiquitination
T307 Phosphorylation
T314 Phosphorylation
T318 Phosphorylation
K319 Ubiquitination
K340 Ubiquitination
K345 Ubiquitination
K367 Ubiquitination
K377 Ubiquitination
K379 Ubiquitination
S395 Phosphorylation
Site PTM Type Enzyme
K199 Ubiquitination

Research Backgrounds

Tissue Specificity:

Synthesis occurs immediately above a small population of matrix cells at the base of the hair bulb and the trichocytes lining the dermal papilla and extends upward through the matrix and ends in the lower part of the cortex of the hair shaft.

Subunit Structure:

Heterotetramer of two type I and two type II keratins.

Family&Domains:

Belongs to the intermediate filament family.

Tissue Specificity:

Synthesis begins slightly higher in the hair shaft than HB1 and HB3 and continues much farther up, ending in the keratogeneous zone.

Subunit Structure:

Heterotetramer of two type I and two type II keratins.

Family&Domains:

Belongs to the intermediate filament family.

Tissue Specificity:

Synthesis begins in the cortex 10-15 cell layers above the apex of the dermal papilla and ends abruptly in the middle of the cortex.

Subunit Structure:

Heterotetramer of two type I and two type II keratins.

Family&Domains:

Belongs to the intermediate filament family.

Tissue Specificity:

Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla. Expressed in malignant lymph node tissue in breast carcinoma tissue.

Subunit Structure:

Heterotetramer of two type I and two type II keratins.

Family&Domains:

Belongs to the intermediate filament family.

Subunit Structure:

Heterotetramer of two type I and two type II keratins.

Family&Domains:

Belongs to the intermediate filament family.

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