Product: K2C1B Antibody
Catalog: DF2555
Description: Rabbit polyclonal antibody to K2C1B
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 61kDa; 62kD(Calculated).
Uniprot: Q7Z794
RRID: AB_2839761

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(80%), Bovine(89%), Horse(80%), Sheep(90%)
Clonality:
Polyclonal
Specificity:
K2C1B Antibody detects endogenous levels of total K2C1B.
RRID:
AB_2839761
Cite Format: Affinity Biosciences Cat# DF2555, RRID:AB_2839761.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK-1B; Cytokeratin-1B; K1B; K2C1B_HUMAN; K77; KB39; Keratin 1B; Keratin, type II cytoskeletal 1b; Keratin-77; KRT1B; Krt77; Type-II keratin Kb39;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q7Z794 K2C1B_HUMAN:

Expressed exclusively in skin.

Sequence:
MSHQFSSQSAFSSMSRRVYSTSSSAGSGGGSPAVGSVCYARGRCGGGGYGIHGRGFGSRSLYNLGGSRSISINLMGRSTSGFCQGGGVGGFGGGRGFGVGSTGAGGFGGGGFGGAGFGTSNFGLGGFGPYCPPGGIQEVTINQSLLEPLHLEVDPEIQRIKTQEREQIMVLNNKFASFIDKVRFLEQQNQVLQTKWELLQQVNTSTGTNNLEPLLENYIGDLRRQVDLLSAEQMRQNAEVRSMQDVVEDYKSKYEDEINKRTGSENDFVVLKKDVDAAYVSKVDLESRVDTLTGEVNFLKYLFLTELSQVQTHISDTNVILSMDNNRSLDLDSIIDAVRTQYELIAQRSKDEAEALYQTKYQELQITAGRHGDDLKNSKMEIAELNRTVQRLQAEISNVKKQIEQMQSLISDAEERGEQALQDAWQKLQDLEEALQQSKEELARLLRDYQAMLGVKLSLDVEIATYRQLLEGEESRMSGELQSHVSISVQNSQVSVNGGAGGGGSYGSGGYGGGSGGGYGGGRSYRGGGARGRSGGGYGSGCGGGGGSYGGSGRSGRGSSRVQIIQTSTNTSHRRILE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
90
Bovine
89
Pig
80
Horse
80
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z794 As Substrate

Site PTM Type Enzyme
S23 Phosphorylation
S27 Phosphorylation
S60 Phosphorylation
S69 Phosphorylation
S71 Phosphorylation
K174 Ubiquitination
S177 Phosphorylation
K181 Ubiquitination
K253 Ubiquitination
S328 Phosphorylation
T465 Phosphorylation
Y466 Phosphorylation
S475 Phosphorylation
S534 Phosphorylation
Y549 Phosphorylation
S552 Phosphorylation

Research Backgrounds

PTMs:

Undergoes deimination of some arginine residues (citrullination).

Tissue Specificity:

Expressed exclusively in skin.

Family&Domains:

Belongs to the intermediate filament family.

Restrictive clause

 

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For Research Use Only.
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