K2C1 Antibody - #DF2554

(3)  
Product: K2C1 Antibody
Catalog: DF2554
Description: Rabbit polyclonal antibody to K2C1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Horse
Mol.Wt.: 67 kDa; 66kD(Calculated).
Uniprot: P04264
RRID: AB_2839760

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(83%)
Clonality:
Polyclonal
Specificity:
K2C1 Antibody detects endogenous levels of total K2C1.
RRID:
AB_2839760
Cite Format: Affinity Biosciences Cat# DF2554, RRID:AB_2839760.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

67 kDa cytokeratin; CK-1; CK1; Cytokeratin 1; Cytokeratin 19; Cytokeratin 8; Cytokeratin-1; EHK; EHK1; epidermolytic hyperkeratosis 1; EPPK; Hair alpha protein; K1; K2C1_HUMAN; KB1; Keratin 1; keratin 1, type II; Keratin 19; Keratin 8; Keratin; keratin, type II cytoskeletal 1; Keratin-1; Krt1; KRT19; KRT1A; KRT8; KRTA; NEPPK; type II cytoskeletal 1; Type-II keratin Kb1;

Immunogens

Immunogen:
Uniprot:

P04264(K2C1_HUMAN) >>Visit HPA database.

Gene(ID):
KRT1(3848)
Expression:
P04264 K2C1_HUMAN:

The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.

Description:
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK
Sequence:
MSRQFSSRSGYRSGGGFSSGSAGIINYQRRTTSSSTRRSGGGGGRFSSCGGGGGSFGAGGGFGSRSLVNLGGSKSISISVARGGGRGSGFGGGYGGGGFGGGGFGGGGFGGGGIGGGGFGGFGSGGGGFGGGGFGGGGYGGGYGPVCPPGGIQEVTINQSLLQPLNVEIDPEIQKVKSREREQIKSLNNQFASFIDKVRFLEQQNQVLQTKWELLQQVDTSTRTHNLEPYFESFINNLRRRVDQLKSDQSRLDSELKNMQDMVEDYRNKYEDEINKRTNAENEFVTIKKDVDGAYMTKVDLQAKLDNLQQEIDFLTALYQAELSQMQTQISETNVILSMDNNRSLDLDSIIAEVKAQYEDIAQKSKAEAESLYQSKYEELQITAGRHGDSVRNSKIEISELNRVIQRLRSEIDNVKKQISNLQQSISDAEQRGENALKDAKNKLNDLEDALQQAKEDLARLLRDYQELMNTKLALDLEIATYRTLLEGEESRMSGECAPNVSVSVSTSHTTISGGGSRGGGGGGYGSGGSSYGSGGGSYGSGGGGGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
83
Rabbit
58
Pig
50
Bovine
50
Sheep
45
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04264 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
S21 Phosphorylation
Y27 Phosphorylation
T31 Phosphorylation
S47 Phosphorylation
S48 Phosphorylation
S66 Phosphorylation
R82 Methylation
S193 Phosphorylation
K197 Acetylation
K197 Ubiquitination
K211 Methylation
S233 Phosphorylation
S254 Phosphorylation
K269 Methylation
K269 Ubiquitination
Y270 Phosphorylation
K276 Methylation
K276 Ubiquitination
Y295 Phosphorylation
T297 Phosphorylation
K298 Acetylation
S344 Phosphorylation
K355 Ubiquitination
Y358 Phosphorylation
K364 Ubiquitination
K366 Ubiquitination
S371 Phosphorylation
Y373 Phosphorylation
Y377 Phosphorylation
T383 Phosphorylation
S394 Phosphorylation
K395 Ubiquitination
S399 Phosphorylation
K417 Ubiquitination
S427 Phosphorylation
K443 Ubiquitination
S491 Phosphorylation
S494 Phosphorylation
S502 Phosphorylation
T507 Phosphorylation
S517 Phosphorylation
R518 Methylation
S530 Phosphorylation
Y532 Phosphorylation
S534 Phosphorylation
Y539 Phosphorylation
S551 Phosphorylation
Y552 Phosphorylation
S558 Phosphorylation
S568 Phosphorylation
Y578 Phosphorylation
Y587 Phosphorylation
S598 Phosphorylation
S599 Phosphorylation
R602 Methylation
S604 Phosphorylation
S608 Phosphorylation
S612 Phosphorylation
R616 Methylation
S618 Phosphorylation
S619 Phosphorylation
S636 Phosphorylation
T638 Phosphorylation
Y639 Phosphorylation

Research Backgrounds

Function:

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

PTMs:

Undergoes deimination of some arginine residues (citrullination).

Subcellular Location:

Cell membrane.
Note: Located on plasma membrane of neuroblastoma NMB7 cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Heterodimer with KRT10. Two heterodimers of KRT1 and KRT10 form a heterotetramer. Interacts with ITGB1 in the presence of RACK1 and SRC, and with RACK1. Interacts with C1QBP; the association represents a cell surface kininogen receptor. Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament.

Family&Domains:

Belongs to the intermediate filament family.

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