Product: KRT31/33A/33B Antibody
Catalog: DF2553
Description: Rabbit polyclonal antibody to KRT31/33A/33B
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 46 kDa; 46kD,47kD(Calculated).
Uniprot: O76009 | Q14525 | Q15323
RRID: AB_2839759

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Bovine(100%), Horse(100%), Dog(90%)
Clonality:
Polyclonal
Specificity:
KRT31/33A/33B Antibody detects endogenous levels of total KRT31/33A/33B.
RRID:
AB_2839759
Cite Format: Affinity Biosciences Cat# DF2553, RRID:AB_2839759.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ha-3I; HA3I; Hair keratin; Hair keratin, type I Ha3-l; Hard keratin, type I,3I; hHa3-I; HHA3-l; HKA3A; K33A; Keratin; Keratin, hair, acidic, 3A; Keratin, type I cuticular Ha3-I; Keratin-33A; Krt1-3; Krt33a; KRTHA3A; KT33A_HUMAN; type I cuticular Ha3-I; type I Ha3-I; HA 3II; HA3II; Hair keratin; Hair keratin type I Ha3 II; Hard keratin type I 3II; hHa3 II; hHa3II; HKA3B; K33B; Keratin 33B; Keratin; Keratin hair acidic 3B; Keratin hair acidic3B; Keratin type I cuticular Ha3 II; Keratin-33B; Keratin33B; KRT 33B; KRT33B; KRTHA 3B; KRTHA3A; KRTHA3B; KT33B_HUMAN; type I cuticular Ha3-II; type I Ha3-II; Type I hair keratin 3B; Ha-1; HA1; Hair Keratin acidic hHa1; Hair keratin; hard keratin, type I, 1; hHa1; HKA1; K1H1_HUMAN; K31; keratin 31; Keratin; keratin, hair, acidic,1; keratin, type I cuticular Ha1; Keratin-31; Krt31; KRTHA1; MGC138630; type I cuticular Ha1; type I Ha1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O76009 KT33A_HUMAN:

Expressed in the hair follicles.

Q15323 K1H1_HUMAN:

Present in scalp but not in hairless skin. Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla.

Sequence:
MSYSCGLPSLSCRTSCSSRPCVPPSCHGCTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELENLIRERSQQQEPLVCASYQSYFKTIEELQQKILCSKSENARLVVQIDNAKLASDDFRTKYETELSLRQLVESDINGLRRILDELTLCRSDLEAQVESLKEELLCLKQNHEQEVNTLRCQLGDRLNVEVDAAPTVDLNQVLNETRSQYEALVETNRREVEQWFATQTEELNKQVVSSSEQLQSYQAEIIELRRTVNALEIELQAQHNLRDSLENTLTESEARYSSQLSQVQRLITNVESQLAEIRSDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCKLPSNPCATTNACDKSTGPCISNPCGLRARCGPCNTFGY

MPYNFCLPSLSCRTSCSSRPCVPPSCHGYTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELENLIRERSQQQEPLLCPSYQSYFKTIEELQQKILCSKSENARLVVQIDNAKLAADDFRTKYQTEQSLRQLVESDINSLRRILDELTLCRSDLEAQMESLKEELLSLKQNHEQEVNTLRCQLGDRLNVEVDAAPAVDLNQVLNETRNQYEALVETNRREVEQWFATQTEELNKQVVSSSEQLQSYQAEIIELRRTVNALEIELQAQHNLRYSLENTLTESEARYSSQLSQVQSLITNVESQLAEIRSDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCKLPSNPCATTNACEKPIGSCVTNPCGPRSRCGPCNTFGY

MPYNFCLPSLSCRTSCSSRPCVPPSCHSCTLPGACNIPANVSNCNWFCEGSFNGSEKETMQFLNDRLASYLEKVRQLERDNAELENLIRERSQQQEPLLCPSYQSYFKTIEELQQKILCTKSENARLVVQIDNAKLAADDFRTKYQTELSLRQLVESDINGLRRILDELTLCKSDLEAQVESLKEELLCLKSNHEQEVNTLRCQLGDRLNVEVDAAPTVDLNRVLNETRSQYEALVETNRREVEQWFTTQTEELNKQVVSSSEQLQSYQAEIIELRRTVNALEIELQAQHNLRDSLENTLTESEARYSSQLSQVQSLITNVESQLAEIRSDLERQNQEYQVLLDVRARLECEINTYRSLLESEDCNLPSNPCATTNACSKPIGPCLSNPCTSCVPPAPCTPCAPRPRCGPCNSFVR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Pig
90
Dog
90
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O76009/Q14525/Q15323 As Substrate

Site PTM Type Enzyme
Y70 Phosphorylation
T109 Phosphorylation
Y268 Phosphorylation
T299 Phosphorylation
Site PTM Type Enzyme
Y70 Phosphorylation
T109 Phosphorylation
S157 Phosphorylation
Y268 Phosphorylation
Site PTM Type Enzyme
Y70 Phosphorylation
S92 Phosphorylation
T109 Phosphorylation
K116 Acetylation
K121 Acetylation
T218 Phosphorylation
Y268 Phosphorylation
T299 Phosphorylation

Research Backgrounds

Tissue Specificity:

Expressed in the hair follicles.

Family&Domains:

Belongs to the intermediate filament family.

Family&Domains:

Belongs to the intermediate filament family.

Tissue Specificity:

Present in scalp but not in hairless skin. Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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