IL16 Antibody - #DF2522

(1)   (3)  
Product: IL16 Antibody
Catalog: DF2522
Description: Rabbit polyclonal antibody to IL16
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 142 kDa; 142kD(Calculated).
Uniprot: Q14005
RRID: AB_2839728

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
IL16 Antibody detects endogenous levels of total IL16.
RRID:
AB_2839728
Cite Format: Affinity Biosciences Cat# DF2522, RRID:AB_2839728.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HGNC:5980; HsT19289; IL-16; IL16; IL16_HUMAN; Interleukin 16 precursor; Interleukin-16; LCF; Lymphocyte chemoattractant factor; Neuronal interleukin 16; NIL16; prIL 16; PrIL16; Prointerleukin 16;

Immunogens

Immunogen:
Uniprot:

Q14005(IL16_HUMAN) >>Visit HPA database.

Gene(ID):
IL16(3603)
Expression:
Q14005 IL16_HUMAN:

Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.

Description:
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.;Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.;Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells
Sequence:
MESHSRAGKSRKSAKFRSISRSLMLCNAKTSDDGSSPDEKYPDPFEISLAQGKEGIFHSSVQLADTSEAGPSSVPDLALASEAAQLQAAGNDRGKTCRRIFFMKESSTASSREKPGKLEAQSSNFLFPKACHQRARSNSTSVNPYCTREIDFPMTKKSAAPTDRQPYSLCSNRKSLSQQLDCPAGKAAGTSRPTRSLSTAQLVQPSGGLQASVISNIVLMKGQAKGLGFSIVGGKDSIYGPIGIYVKTIFAGGAAAADGRLQEGDEILELNGESMAGLTHQDALQKFKQAKKGLLTLTVRTRLTAPPSLCSHLSPPLCRSLSSSTCITKDSSSFALESPSAPISTAKPNYRIMVEVSLQKEAGVGLGIGLCSVPYFQCISGIFVHTLSPGSVAHLDGRLRCGDEIVEISDSPVHCLTLNEVYTILSHCDPGPVPIIVSRHPDPQVSEQQLKEAVAQAVENTKFGKERHQWSLEGVKRLESSWHGRPTLEKEREKNSAPPHRRAQKVMIRSSSDSSYMSGSPGGSPGSGSAEKPSSDVDISTHSPSLPLAREPVVLSIASSRLPQESPPLPESRDSHPPLRLKKSFEILVRKPMSSKPKPPPRKYFKSDSDPQKSLEERENSSCSSGHTPPTCGQEARELLPLLLPQEDTAGRSPSASAGCPGPGIGPQTKSSTEGEPGWRRASPVTQTSPIKHPLLKRQARMDYSFDTTAEDPWVRISDCIKNLFSPIMSENHGHMPLQPNASLNEEEGTQGHPDGTPPKLDTANGTPKVYKSADSSTVKKGPPVAPKPAWFRQSLKGLRNRASDPRGLPDPALSTQPAPASREHLGSHIRASSSSSSIRQRISSFETFGSSQLPDKGAQRLSLQPSSGEAAKPLGKHEEGRFSGLLGRGAAPTLVPQQPEQVLSSGSPAASEARDPGVSESPPPGRQPNQKTLPPGPDPLLRLLSTQAEESQGPVLKMPSQRARSFPLTRSQSCETKLLDEKTSKLYSISSQVSSAVMKSLLCLPSSISCAQTPCIPKEGASPTSSSNEDSAANGSAETSALDTGFSLNLSELREYTEGLTEAKEDDDGDHSSLQSGQSVISLLSSEELKKLIEEVKVLDEATLKQLDGIHVTILHKEEGAGLGFSLAGGADLENKVITVHRVFPNGLASQEGTIQKGNEVLSINGKSLKGTTHHDALAILRQAREPRQAVIVTRKLTPEAMPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
92
Zebrafish
83
Rabbit
83
Xenopus
75
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14005 As Substrate

Site PTM Type Enzyme
S139 Phosphorylation
S175 Phosphorylation
S177 Phosphorylation
T298 Phosphorylation
T301 Phosphorylation
S322 Phosphorylation
T325 Phosphorylation
S331 Phosphorylation
S471 Phosphorylation
S480 Phosphorylation
S481 Phosphorylation
S566 Phosphorylation
S572 Phosphorylation
S584 Phosphorylation
S614 Phosphorylation
T631 Phosphorylation
S683 Phosphorylation
S705 Phosphorylation
S743 Phosphorylation P68400 (CSNK2A1)
T757 Phosphorylation P06493 (CDK1)
Y771 Phosphorylation
K772 Ubiquitination
K788 Ubiquitination
S795 Phosphorylation
S804 Phosphorylation
S833 Phosphorylation
S834 Phosphorylation
S835 Phosphorylation
S836 Phosphorylation
S844 Phosphorylation
S845 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
T848 Phosphorylation
S851 Phosphorylation
S852 Phosphorylation
K857 Ubiquitination
S863 Phosphorylation
R882 Methylation
S884 Phosphorylation
R889 Methylation
S920 Phosphorylation
S922 Phosphorylation
T933 Phosphorylation
S946 Phosphorylation
T947 Phosphorylation
S952 Phosphorylation
S961 Phosphorylation
S966 Phosphorylation
T970 Phosphorylation
S972 Phosphorylation
S974 Phosphorylation
K978 Methylation
S989 Phosphorylation
S995 Phosphorylation
S996 Phosphorylation
S1001 Phosphorylation
S1074 Phosphorylation
S1247 Phosphorylation
S1254 Phosphorylation
K1259 Ubiquitination
K1305 Ubiquitination
S1320 Phosphorylation
K1324 Ubiquitination

Research Backgrounds

Function:

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.

Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.

Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

PTMs:

Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.

Subcellular Location:

Secreted.

Cytoplasm.

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.

Subunit Structure:

Homotetramer (Probable). According to the formation of a homotetrameric protein complex is not required for the chemo-attractant function. Isoform 3 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GRIN2A. Isoform 3 interacts with GABPB1. Isoform 3 interacts (via PDZ 3 domain) with HDAC3. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C (By similarity).

(Microbial infection) Isoform 3 interacts with HTLV-1 tax.

References

1). Single-cell RNA sequencing reveals cell-cell communication and potential biomarker in sepsis and septic shock patients. International immunopharmacology, 2024 (PubMed: 38593502) [IF=5.6]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.