IL15 Antibody - #DF2521

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Product: IL15 Antibody
Catalog: DF2521
Description: Rabbit polyclonal antibody to IL15
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Horse, Rabbit
Mol.Wt.: 18 kDa; 18kD(Calculated).
Uniprot: P40933
RRID: AB_2839727

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(100%), Rabbit(88%)
Clonality:
Polyclonal
Specificity:
IL15 Antibody detects endogenous levels of total IL15.
RRID:
AB_2839727
Cite Format: Affinity Biosciences Cat# DF2521, RRID:AB_2839727.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IL 15; IL-15; IL15; IL15_HUMAN; Interleukin 15; Interleukin-15; Interleukin15; MGC9721;

Immunogens

Immunogen:
Uniprot:

P40933(IL15_HUMAN) >>Visit HPA database.

Gene(ID):
IL15(3600)
Expression:
P40933 IL15_HUMAN:

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.

Description:
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
Sequence:
MRISKPHLRSISIQCYLCLLLNSHFLTEAGIHVFILGCFSAGLPKTEANWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Rabbit
88
Dog
75
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40933 As Substrate

Site PTM Type Enzyme
T46 Phosphorylation
S55 Phosphorylation

Research Backgrounds

Function:

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.

Subcellular Location:

Secreted.

Cytoplasm. Nucleus.
Note: IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.

Family&Domains:

Belongs to the IL-15/IL-21 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Intestinal immune network for IgA production.   (View pathway)

References

1). IL-15 Participates in the Pathogenesis of Polycystic Ovary Syndrome by Affecting the Activity of Granulosa Cells. Frontiers in Endocrinology, 2022 (PubMed: 35250857) [IF=5.2]

Application: IF/ICC    Species: Mice    Sample: ovarian tissues

Figure 3 Higher level of IL-15 in PCOS model mice. (A, B). ELISA detection of IL-15 in the serum and ovarian tissue homogenate of two groups of mice (n=3 per group, P=0.2146, 0.0026; R=0.3044, 0.9179 respectively); (C) PCR detection of IL-15 in ovarian tissues in the two groups (CON n=5, PCOS n=6, P=0.0032, R=0.5984); (D) PCR detection of IL-15 in adipose tissue in the two groups (n=6 per group, P=0.0103, R=0.5819); (E) IHC analysis of IL-15 in the ovaries of two groups of mice. The brown part represents the part of IL-15 expression. Bar=50um, 40X. Images are representative of three independent experiments with similar results (P=0.0055, R=0.5541); (F), Blue shows the nucleus (DAPI), green fluorescence shows ovarian granulosa cells marker FSHR (TSA 520, excitation and emission wavelengths similar to FITC), red shows IL-15(TSA 650, excitation and emission wavelengths similar to AF610). Bar=250um. *P < 0.05, **P < 0.01 versus the control.

Application: IHC    Species: Mice    Sample: ovarian tissues

Figure 3 Higher level of IL-15 in PCOS model mice. (A, B). ELISA detection of IL-15 in the serum and ovarian tissue homogenate of two groups of mice (n=3 per group, P=0.2146, 0.0026; R=0.3044, 0.9179 respectively); (C) PCR detection of IL-15 in ovarian tissues in the two groups (CON n=5, PCOS n=6, P=0.0032, R=0.5984); (D) PCR detection of IL-15 in adipose tissue in the two groups (n=6 per group, P=0.0103, R=0.5819); (E) IHC analysis of IL-15 in the ovaries of two groups of mice. The brown part represents the part of IL-15 expression. Bar=50um, 40X. Images are representative of three independent experiments with similar results (P=0.0055, R=0.5541); (F), Blue shows the nucleus (DAPI), green fluorescence shows ovarian granulosa cells marker FSHR (TSA 520, excitation and emission wavelengths similar to FITC), red shows IL-15(TSA 650, excitation and emission wavelengths similar to AF610). Bar=250um. *P < 0.05, **P < 0.01 versus the control.

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