GBP2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2 cells(H2O2 treatment), using GBP2 Antibody.

Product:	GBP2 Antibody
Catalog:	DF2499
Description:	Rabbit polyclonal antibody to GBP2
Application:	WB IHC
Reactivity:	Human, Mouse
Mol.Wt.:	67 kDa; 67kD(Calculated).
Uniprot:	P32456
RRID:	AB_2839705

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

GBP2 Antibody detects endogenous levels of total GBP2.

RRID:

AB_2839705
Cite Format: Affinity Biosciences Cat# DF2499, RRID:AB_2839705.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GBP 2; GTP binding protein 2; Guanine nucleotide binding protein 2; Guanylate binding protein 2 interferon inducible; HuGBP 2; HuGBP2; Interferon induced guanylate binding protein 2;

Immunogens

Immunogen:

Uniprot:

P32456(GBP2_HUMAN) >>Visit HPA database.

Gene(ID):

GBP2(2634)

Sequence:

P32456 GBP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPEINLPGPMSLIDNTKGQLVVNPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKKNGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDIEKGDNENDSWIFALAILLSSTFVYNSMGTINQQAMDQLHYVTELTDRIKANSSPGNNSVDDSADFVSFFPAFVWTLRDFTLELEVDGEPITADDYLELSLKLRKGTDKKSKSFNDPRLCIRKFFPKRKCFVFDWPAPKKYLAHLEQLKEEELNPDFIEQVAEFCSYILSHSNVKTLSGGIPVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLSEKEKAIEVERIKAESAEAAKKMLEEIQKKNEEMMEQKEKSYQEHVKQLTEKMERDRAQLMAEQEKTLALKLQEQERLLKEGFENESKRLQKDIWDIQMRSKSLEPICNIL

PTMs - P32456 As Substrate

P32456

Site	PTM Type	Source
S52	Phosphorylation	Uniprot
K242	Acetylation	Uniprot
K242	Ubiquitination	Uniprot
Y333	Phosphorylation	Uniprot
Y446	Phosphorylation	Uniprot
K483	Acetylation	Uniprot
K485	Acetylation	Uniprot
K501	Acetylation	Uniprot

Research Backgrounds

P32456_GBP2_HUMAN

Function:

Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP. Exhibits antiviral activity against influenza virus. Promotes oxidative killing and delivers antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes (By similarity).

PTMs:

Isoprenylation is required for proper subcellular location.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Golgi apparatus membrane. Membrane>Lipid-anchor.
Note: GBP2-GBP5 dimers localize to the Golgi apparatus.

Subunit Structure:

Homodimer; homodimerization occurs upon GTP-binding and is required for the association with membranous structures. Heterodimer with other family members, including GBP1, GBP3, GBP4 and GBP5.

Family&Domains:

Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GBP2 Antibody - #DF2499