DOT1L Antibody - #DF2490

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Product: DOT1L Antibody
Catalog: DF2490
Description: Rabbit polyclonal antibody to DOT1L
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep
Mol.Wt.: 185 kDa.; 165kD(Calculated).
Uniprot: Q8TEK3
RRID: AB_2839696

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(90%), Sheep(90%)
Clonality:
Polyclonal
Specificity:
DOT1L Antibody detects endogenous levels of total DOT1L.
RRID:
AB_2839696
Cite Format: Affinity Biosciences Cat# DF2490, RRID:AB_2839696.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Disrupter of telomere silencing protein 1; DOT 1; DOT1; DOT1 like; DOT1 like histone H3 methyltransferase (S. cerevisiae); DOT1 like histone H3 methyltransferase; DOT1 like histone H3K79 methyltransferase; DOT1 like protein; DOT1, S. cerevisiae, homolog of; DOT1-like protein; DOT1L; DOT1L_HUMAN; EC 2.1.1.43; H3 K79 HMTase; H3 lysine-79 specific; H3-K79-HMTase; Histone H3 K79 methyltransferase; Histone H3 methyltransferase; Histone H3 methyltransferase DOT1-like; Histone H3-K79 methyltransferase; Histone lysine N methyltransferase H3 lysine 79 specific; Histone methyltransferase DOT1L; Histone-lysine N-methyltransferase; KIAA1814; KMT4; Lysine N methyltransferase 4; Lysine N-methyltransferase 4;

Immunogens

Immunogen:
Uniprot:

Q8TEK3(DOT1L_HUMAN) >>Visit HPA database.

Gene(ID):
DOT1L(84444)
Sequence:
MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTDDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKSNAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMNTANPERKPKKNQTALDALHAQTVSQTAASSPQDAYRSPHSPFYQLPPSVQRHSPNPLLVAPTPPALQKLLESFKIQYLQFLAYTKTPQYKASLQELLGQEKEKNAQLLGAAQQLLSHCQAQKEEIRRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQSEQLEQDNRALRGQSLQLLKARCEELQLDWATLSLEKLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDDALSLHLRGKGALGRELEPDASRLHLELDCTKFSLPHLSSMSPELSMNGQAAGYELCGVLSRPSSKQNTPQYLASPLDQEVVPCTPSHVGRPRLEKLSGLAAPDYTRLSPAKIVLRRHLSQDHTVPGRPAASELHSRAEHTKENGLPYQSPSVPGSMKLSPQDPRPLSPGALQLAGEKSSEKGLRERAYGSSGELITSLPISIPLSTVQPNKLPVSIPLASVVLPSRAERARSTPSPVLQPRDPSSTLEKQIGANAHGAGSRSLALAPAGFSYAGSVAISGALAGSPASLTPGAEPATLDESSSSGSLFATVGSRSSTPQHPLLLAQPRNSLPASPAHQLSSSPRLGGAAQGPLPEASKGDLPSDSGFSDPESEAKRRIVFTITTGAGSAKQSPSSKHSPLTASARGDCVPSHGQDSRRRGRRKRASAGTPSLSAGVSPKRRALPSVAGLFTQPSGSPLNLNSMVSNINQPLEITAISSPETSLKSSPVPYQDHDQPPVLKKERPLSQTNGAHYSPLTSDEEPGSEDEPSSARIERKIATISLESKSPPKTLENGGGLAGRKPAPAGEPVNSSKWKSTFSPISDIGLAKSADSPLQASSALSQNSLFTFRPALEEPSADAKLAAHPRKGFPGSLSGADGLSPGTNPANGCTFGGGLAADLSLHSFSDGASLPHKGPEAAGLSSPLSFPSQRGKEGSDANPFLSKRQLDGLAGLKGEGSRGKEAGEGGLPLCGPTDKTPLLSGKAAKARDREVDLKNGHNLFISAAAVPPGSLLSGPGLAPAASSAGGAASSAQTHRSFLGPFPPGPQFALGPMSLQANLGSVAGSSVLQSLFSSVPAAAGLVHVSSAATRLTNSHAMGSFSGVAGGTVGGN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
90
Sheep
90
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TEK3 As Substrate

Site PTM Type Enzyme
S67 Phosphorylation
Y78 Phosphorylation
T104 Phosphorylation
K270 Ubiquitination
S297 Phosphorylation
S302 Phosphorylation
S351 Phosphorylation
T355 Phosphorylation
S374 Phosphorylation
K387 Acetylation
S390 Phosphorylation
K397 Acetylation
K398 Acetylation
K401 Acetylation
S442 Phosphorylation
S448 Phosphorylation
S458 Phosphorylation
Y461 Phosphorylation
S471 Phosphorylation
T480 Phosphorylation
K508 Ubiquitination
K519 Ubiquitination
K521 Ubiquitination
K557 Ubiquitination
K569 Ubiquitination
S596 Phosphorylation
K601 Ubiquitination
S628 Phosphorylation
S631 Phosphorylation
K660 Ubiquitination
K676 Acetylation
S727 Phosphorylation
S731 Phosphorylation
K732 Ubiquitination
T735 Phosphorylation
Y738 Phosphorylation
S741 Phosphorylation
T751 Phosphorylation
S753 Phosphorylation
S764 Phosphorylation
Y771 Phosphorylation
T772 Phosphorylation
S775 Phosphorylation
S786 Phosphorylation
Y814 Phosphorylation
S822 Phosphorylation
S826 Phosphorylation
S834 Phosphorylation
S846 Phosphorylation
Y855 Phosphorylation
S857 Phosphorylation
S858 Phosphorylation
S882 Phosphorylation
S892 Phosphorylation
S899 Phosphorylation
T900 Phosphorylation
S902 Phosphorylation
S982 Phosphorylation
T984 Phosphorylation
S997 Phosphorylation
S1001 Phosphorylation
S1007 Phosphorylation
S1008 Phosphorylation
S1009 Phosphorylation
S1030 Phosphorylation
S1035 Phosphorylation
S1039 Phosphorylation
S1055 Phosphorylation
S1059 Phosphorylation
S1065 Phosphorylation
S1093 Phosphorylation
T1096 Phosphorylation
S1100 Phosphorylation
S1104 Phosphorylation
S1123 Phosphorylation
S1153 Phosphorylation
T1206 Phosphorylation
S1208 Phosphorylation
S1213 Phosphorylation
T1217 Phosphorylation
S1246 Phosphorylation
S1256 Phosphorylation
S1259 Phosphorylation
S1268 Phosphorylation
S1349 Phosphorylation
K1359 Methylation
S1362 Phosphorylation
S1384 Phosphorylation
T1403 Phosphorylation

Research Backgrounds

Function:

Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with MLLT10.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Metabolism > Amino acid metabolism > Lysine degradation.

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For Research Use Only.
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