BIN2 Antibody - #DF2479

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Product: BIN2 Antibody
Catalog: DF2479
Description: Rabbit polyclonal antibody to BIN2
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 62 kDa; 62kD(Calculated).
Uniprot: Q9UBW5
RRID: AB_2839685

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
BIN2 Antibody detects endogenous levels of total BIN2.
RRID:
AB_2839685
Cite Format: Affinity Biosciences Cat# DF2479, RRID:AB_2839685.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BIN2; BIN2_HUMAN; BRAP 1; Breast cancer associated protein BRAP1; Breast cancer-associated protein 1; Bridging integrator 2;

Immunogens

Immunogen:
Uniprot:

Q9UBW5(BIN2_HUMAN) >>Visit HPA database.

Gene(ID):
BIN2(51411)
Expression:
Q9UBW5 BIN2_HUMAN:

Detected in natural killer cells (at protein level). Highest level expression seen in spleen and peripheral blood leukocytes and is also expressed at high levels in thymus, colon and placenta, suggesting preferential expression in hematopoietic tissues.

Sequence:
MAEGKAGGAAGLFAKQVQKKFSRAQEKVLQKLGKAVETKDERFEQSASNFYQQQAEGHKLYKDLKNFLSAVKVMHESSKRVSETLQEIYSSEWDGHEELKAIVWNNDLLWEDYEEKLADQAVRTMEIYVAQFSEIKERIAKRGRKLVDYDSARHHLEAVQNAKKKDEAKTAKAEEEFNKAQTVFEDLNQELLEELPILYNSRIGCYVTIFQNISNLRDVFYREMSKLNHNLYEVMSKLEKQHSNKVFVVKGLSSSSRRSLVISPPVRTATVSSPLTSPTSPSTLSLKSESESVSATEDLAPDAAQGEDNSEIKELLEEEEIEKEGSEASSSEEDEPLPACNGPAQAQPSPTTERAKSQEEVLPSSTTPSPGGALSPSGQPSSSATEVVLRTRTASEGSEQPKKRASIQRTSAPPSRPPPPRATASPRPSSGNIPSSPTASGGGSPTSPRASLGTGTASPRTSLEVSPNPEPPEKPVRTPEAKENENIHNQNPEELCTSPTLMTSQVASEPGEAKKMEDKEKDNKLISANSSEGQDQLQVSMVPENNNLTAPEPQEEVSTSENPQL

PTMs - Q9UBW5 As Substrate

Site PTM Type Enzyme
K5 Acetylation
K5 Ubiquitination
K19 Ubiquitination
K20 Acetylation
K27 Acetylation
K59 Ubiquitination
K65 Ubiquitination
S69 Phosphorylation
S77 Phosphorylation
S78 Phosphorylation
K79 Ubiquitination
T124 Phosphorylation
K145 Ubiquitination
Y149 Phosphorylation
S151 Phosphorylation
K163 Ubiquitination
K164 Acetylation
K165 Acetylation
K169 Acetylation
S225 Phosphorylation
Y232 Phosphorylation
S236 Phosphorylation
K237 Ubiquitination
K250 Ubiquitination
S259 Phosphorylation
S263 Phosphorylation
T268 Phosphorylation
T270 Phosphorylation
S272 Phosphorylation
S273 Phosphorylation
T276 Phosphorylation
S277 Phosphorylation
T279 Phosphorylation
S280 Phosphorylation
S285 Phosphorylation
S310 Phosphorylation
S349 Phosphorylation
T352 Phosphorylation
S357 Phosphorylation
T366 Phosphorylation
S369 Phosphorylation
S375 Phosphorylation
S377 Phosphorylation
T385 Phosphorylation
T393 Phosphorylation
S395 Phosphorylation
S398 Phosphorylation
S406 Phosphorylation
T410 Phosphorylation
S411 Phosphorylation
S415 Phosphorylation
T423 Phosphorylation
S425 Phosphorylation
S429 Phosphorylation
S430 Phosphorylation
S435 Phosphorylation
S436 Phosphorylation
T438 Phosphorylation
S440 Phosphorylation
S444 Phosphorylation
T446 Phosphorylation
S447 Phosphorylation
S451 Phosphorylation
T454 Phosphorylation
T456 Phosphorylation
S458 Phosphorylation
T461 Phosphorylation
S462 Phosphorylation
S466 Phosphorylation
T478 Phosphorylation
S498 Phosphorylation

Research Backgrounds

Function:

Promotes cell motility and migration, probably via its interaction with the cell membrane and with podosome proteins that mediate interaction with the cytoskeleton. Modulates membrane curvature and mediates membrane tubulation. Plays a role in podosome formation. Inhibits phagocytosis.

Subcellular Location:

Cytoplasm. Cell projection>Podosome membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cell cortex. Cell projection>Phagocytic cup.
Note: Associates with membranes enriched in phosphoinositides. Detected in the actin-rich cell cortex at the leading edge of migrating cells. Detected at podosomes, at an actin-rich ring-like structure.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in natural killer cells (at protein level). Highest level expression seen in spleen and peripheral blood leukocytes and is also expressed at high levels in thymus, colon and placenta, suggesting preferential expression in hematopoietic tissues.

Subunit Structure:

Homodimer. Interacts with BIN1. Interacts with ARHGEF6 (via SH3 domain), ARHGEF7 (via SH3 domain), SH3GL1, SH3GL2 and SH3GL3. Identified in a complex with ARHGEF6 and GIT2.

Family&Domains:

The BAR domain mediates dimerization and interaction with membranes enriched in phosphatidylinositides.

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