HNF-6 Antibody | Affinity Biosciences

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Western blot analysis of extracts from RAW264.

Product:	HNF-6 Antibody
Catalog:	DF2478
Description:	Rabbit polyclonal antibody to HNF-6
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	51 kDa; 51kD(Calculated).
Uniprot:	Q9UBC0
RRID:	AB_2839684

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

HNF-6 Antibody detects endogenous levels of total HNF-6.

RRID:

AB_2839684
Cite Format: Affinity Biosciences Cat# DF2478, RRID:AB_2839684.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

hepatocyte nuclear factor 6 alpha; Hepatocyte nuclear factor 6; HNF 6; HNF-6; HNF6_HUMAN; HNF6A; One cut domain family member 1; One cut homeobox 1; Onecut1;

Immunogens

Immunogen:

Uniprot:

Q9UBC0(HNF6_HUMAN) >>Visit HPA database.

Gene(ID):

ONECUT1(3175)

Expression:

Q9UBC0 HNF6_HUMAN:

Highly expressed in liver; lower expression in testis and skin.

Description:

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.

Sequence:

Q9UBC0 HNF6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNAQLTMEAIGELHGVSHEPVPAPADLLGGSPHARSSVAHRGSHLPPAHPRSMGMASLLDGGSGGGDYHHHHRAPEHSLAGPLHPTMTMACETPPGMSMPTTYTTLTPLQPLPPISTVSDKFPHHHHHHHHHHHPHHHQRLAGNVSGSFTLMRDERGLASMNNLYTPYHKDVAGMGQSLSPLSSSGLGSIHNSQQGLPHYAHPGAAMPTDKMLTPNGFEAHHPAMLGRHGEQHLTPTSAGMVPINGLPPHHPHAHLNAQGHGQLLGTAREPNPSVTGAQVSNGSNSGQMEEINTKEVAQRITTELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMWKWLQEPEFQRMSALRLAACKRKEQEHGKDRGNTPKKPRLVFTDVQRRTLHAIFKENKRPSKELQITISQQLGLELSTVSNFFMNARRRSLDKWQDEGSSNSGNSSSSSSTCTKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Horse

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBC0 As Substrate

Q9UBC0

Site	PTM Type	Source
S31	Phosphorylation	Uniprot
Y68	Phosphorylation	Uniprot
Y168	Phosphorylation	Uniprot
Y308	Phosphorylation	Uniprot
T326	Phosphorylation	Uniprot
S440	Phosphorylation	Uniprot
S450	Phosphorylation	Uniprot
S455	Phosphorylation	Uniprot

Research Backgrounds

Q9UBC0_HNF6_HUMAN

Function:

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.

Subcellular Location:

Nucleus.

Tissue Specificity:

Highly expressed in liver; lower expression in testis and skin.

Subunit Structure:

Binds DNA as a monomer.

Family&Domains:

Belongs to the CUT homeobox family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

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HNF-6 Antibody - #DF2478