BCL11B Antibody - #DF2464

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Product: BCL11B Antibody
Catalog: DF2464
Description: Rabbit polyclonal antibody to BCL11B
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 95 kDa; 96kD(Calculated).
Uniprot: Q9C0K0
RRID: AB_2839670

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
BCL11B Antibody detects endogenous levels of total BCL11B.
RRID:
AB_2839670
Cite Format: Affinity Biosciences Cat# DF2464, RRID:AB_2839670.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATL1 alpha; ATL1; ATL1 beta; ATL1 gamma; ATL1-delta; B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein; B cell lymphoma/leukemia 11B; B-cell CLL/lymphoma 11B (zinc finger protein); B-cell CLL/lymphoma 11B; B-cell lymphoma/leukemia 11B; BC11B_HUMAN; BCL-11B; Bcl11b; BCL11B/TRDC fusion; COUP TF interacting protein 2; COUP-TF interacting protein 2; COUP-TF-interacting protein 2; Ctip 2; CTIP-2; CTIP2; hRIT1 alpha; hRit1; Radiation induced tumor suppressor gene 1; Radiation induced tumor suppressor gene 1 protein; Radiation-induced tumor suppressor gene 1 protein; Rit 1; Rit1; zinc finger protein hRit1 alpha; ZNF856B;

Immunogens

Immunogen:
Uniprot:

Q9C0K0(BC11B_HUMAN) >>Visit HPA database.

Gene(ID):
BCL11B(64919)
Expression:
Q9C0K0 BC11B_HUMAN:

Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.

Description:
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
Sequence:
MSRRKQGNPQHLSQRELITPEADHVEAAILEEDEGLEIEEPSGLGLMVGGPDPDLLTCGQCQMNFPLGDILVFIEHKRKQCGGSLGACYDKALDKDSPPPSSRSELRKVSEPVEIGIQVTPDEDDHLLSPTKGICPKQENIAGPCRPAQLPAVAPIAASSHPHSSVITSPLRALGALPPCLPLPCCSARPVSGDGTQGEGQTEAPFGCQCQLSGKDEPSSYICTTCKQPFNSAWFLLQHAQNTHGFRIYLEPGPASSSLTPRLTIPPPLGPEAVAQSPLMNFLGDSNPFNLLRMTGPILRDHPGFGEGRLPGTPPLFSPPPRHHLDPHRLSAEEMGLVAQHPSAFDRVMRLNPMAIDSPAMDFSRRLRELAGNSSTPPPVSPGRGNPMHRLLNPFQPSPKSPFLSTPPLPPMPPGGTPPPQPPAKSKSCEFCGKTFKFQSNLIVHRRSHTGEKPYKCQLCDHACSQASKLKRHMKTHMHKAGSLAGRSDDGLSAASSPEPGTSELAGEGLKAADGDFRHHESDPSLGHEPEEEDEEEEEEEEELLLENESRPESSFSMDSELSRNRENGGGGVPGVPGAGGGAAKALADEKALVLGKVMENVGLGALPQYGELLADKQKRGAFLKRAAGGGDAGDDDDAGGCGDAGAGGAVNGRGGGFAPGTEPFPGLFPRKPAPLPSPGLNSAAKRIKVEKDLELPPAALIPSENVYSQWLVGYAASRHFMKDPFLGFTDARQSPFATSSEHSSENGSLRFSTPPGDLLDGGLSGRSGTASGGSTPHLGGPGPGRPSSKEGRRSDTCEYCGKVFKNCSNLTVHRRSHTGERPYKCELCNYACAQSSKLTRHMKTHGQIGKEVYRCDICQMPFSVYSTLEKHMKKWHGEHLLTNDVKIEQAERS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
91
Xenopus
82
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9C0K0 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
S13 Phosphorylation
K79 Ubiquitination
S84 Phosphorylation
Y89 Phosphorylation
K91 Ubiquitination
K95 Ubiquitination
S97 Phosphorylation
K108 Ubiquitination
T120 Phosphorylation
S129 Phosphorylation
T131 Phosphorylation
K132 Ubiquitination
S169 Phosphorylation
K215 Ubiquitination
S256 Phosphorylation
S257 Phosphorylation
S258 Phosphorylation
T260 Phosphorylation
R262 Methylation
S277 Phosphorylation
S286 Phosphorylation
R293 Methylation
R300 Methylation
T313 Phosphorylation
S318 Phosphorylation
R322 Methylation
S331 Phosphorylation
S358 Phosphorylation
S364 Phosphorylation
S374 Phosphorylation
S375 Phosphorylation
T376 Phosphorylation
S381 Phosphorylation
S398 Phosphorylation
S401 Phosphorylation
S405 Phosphorylation
T406 Phosphorylation
T417 Phosphorylation
K427 Ubiquitination
K434 Acetylation
K434 Ubiquitination
T450 Phosphorylation
K456 Ubiquitination
S465 Phosphorylation
K469 Ubiquitination
K480 Ubiquitination
S483 Phosphorylation
S488 Phosphorylation
S493 Phosphorylation
S496 Phosphorylation
S497 Phosphorylation
T502 Phosphorylation
S522 Phosphorylation
S550 Phosphorylation
S557 Phosphorylation
S560 Phosphorylation
K585 Ubiquitination
K591 Ubiquitination
K597 Ubiquitination
K617 Ubiquitination
K625 Ubiquitination
T662 Phosphorylation
R671 Methylation
K672 Ubiquitination
S678 Phosphorylation
K686 Ubiquitination
K689 Ubiquitination
K723 Ubiquitination
T730 Phosphorylation
S735 Phosphorylation
T739 Phosphorylation
S749 Phosphorylation
R751 Methylation
S753 Phosphorylation
T754 Phosphorylation
S765 Phosphorylation
S768 Phosphorylation
S772 Phosphorylation
S775 Phosphorylation
T776 Phosphorylation
S788 Phosphorylation
S789 Phosphorylation
K790 Ubiquitination
S795 Phosphorylation
T797 Phosphorylation
K803 Ubiquitination
K806 Ubiquitination
S809 Phosphorylation
T819 Phosphorylation
K825 Ubiquitination
K838 Ubiquitination
K844 Ubiquitination
K851 Acetylation
K851 Ubiquitination
K871 Ubiquitination
K875 Ubiquitination
K887 Ubiquitination
S894 Phosphorylation

Research Backgrounds

Function:

Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals. Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of the receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus. Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes. Tumor-suppressor that represses transcription through direct, TFCOUP2-independent binding to a GC-rich response element (By similarity). May also function in the P53-signaling pathway (By similarity).

PTMs:

Sumoylated with SUMO1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.

Subunit Structure:

Interacts with TFCOUP1, SIRT1, ARP1 and EAR2 (By similarity). Interacts with EP300; the interaction is detected in activated T-lymphocytes, but not under resting conditions.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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