Product: EBP2 Antibody
Catalog: DF2454
Description: Rabbit polyclonal antibody to EBP2
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: Q99848
RRID: AB_2839660

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%), Chicken(82%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
EBP2 Antibody detects endogenous levels of total EBP2.
RRID:
AB_2839660
Cite Format: Affinity Biosciences Cat# DF2454, RRID:AB_2839660.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EBNA1-binding protein 2; ebna1bp2; EBP2; EBP2_HUMAN; NOBP; nuclear FGF3 binding protein; Nucleolar protein p40; P40; Probable rRNA processing protein EBP2; Probable rRNA-processing protein ebp2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99848 EBP2_HUMAN:

Ubiquitous.

Description:
Required for the processing of the 27S pre-rRNA.
Sequence:
MDTPPLSDSESESDESLVTDRELQDAFSRGLLKPGLNVVLEGPKKAVNDVNGLKQCLAEFKRDLEWVERLDVTLGPVPEIGGSEAPAPQNKDQKAVDPEDDFQREMSFYRQAQAAVLAVLPRLHQLKVPTKRPTDYFAEMAKSDLQMQKIRQKLQTKQAAMERSEKAKQLRALRKYGKKVQTEVLQKRQQEKAHMMNAIKKYQKGFSDKLDFLEGDQKPLAQRKKAGAKGQQMRKGPSAKRRYKNQKFGFGGKKKGSKWNTRESYDDVSSFRAKTAHGRGLKRPGKKGSNKRPGKRTREKMKNRTH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Xenopus
90
Chicken
82
Zebrafish
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99848 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T3 Phosphorylation
S7 Phosphorylation
S9 Phosphorylation
S11 Phosphorylation
S13 Phosphorylation
S16 Phosphorylation
T19 Phosphorylation
K33 Ubiquitination
K54 Ubiquitination
K61 Acetylation
T73 Phosphorylation
K94 Ubiquitination
K131 Ubiquitination
Y136 Phosphorylation
S143 Phosphorylation
K149 Ubiquitination
K157 Ubiquitination
K166 Acetylation
K168 Acetylation
K175 Acetylation
K179 Acetylation
K179 Ubiquitination
T182 Phosphorylation O14757 (CHEK1)
K187 Acetylation
K187 Ubiquitination
K192 Ubiquitination
K201 Ubiquitination
S207 Phosphorylation
K209 Acetylation
K209 Ubiquitination
K218 Sumoylation
K218 Ubiquitination
K224 Methylation
K235 Acetylation
K247 Ubiquitination
K253 Acetylation
K258 Ubiquitination
S264 Phosphorylation
Y265 Phosphorylation
S270 Phosphorylation

Research Backgrounds

Function:

Required for the processing of the 27S pre-rRNA.

Subcellular Location:

Nucleus>Nucleolus.
Note: Associated with the nucleolus in an RNA-dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Specifically interacts with EBV EBNA1. The EBNA1-EBP2 interaction is important for the stable segregation of EBV episomes during cell division. Interacts with WDR46.

Family&Domains:

Belongs to the EBP2 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.