VSIG2 Antibody - #DF2446

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Product: VSIG2 Antibody
Catalog: DF2446
Description: Rabbit polyclonal antibody to VSIG2
Application: WB IHC
Reactivity: Human, Rat
Prediction: Horse, Sheep, Dog
Mol.Wt.: 34 kDa; 34kD(Calculated).
Uniprot: Q96IQ7
RRID: AB_2839652

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Horse(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
VSIG2 Antibody detects endogenous levels of total VSIG2.
RRID:
AB_2839652
Cite Format: Affinity Biosciences Cat# DF2446, RRID:AB_2839652.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2210413P10Rik; cortical thymocyte receptor (X. laevis CTX) like; Cortical thymocyte-like protein; CT-like protein; CTH; Ctm; CTXL; OTTHUMP00000197171; OTTHUMP00000197172; V set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; VSIG2; VSIG2_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q96IQ7(VSIG2_HUMAN) >>Visit HPA database.

Gene(ID):
VSIG2(23584)
Expression:
Q96IQ7 VSIG2_HUMAN:

Highly expressed in stomach, colon, prostate, trachea and thyroid glands and weakly in bladder and lung.

Sequence:
MAELPGPFLCGALLGFLCLSGLAVEVKVPTEPLSTPLGKTAELTCTYSTSVGDSFALEWSFVQPGKPISESHPILYFTNGHLYPTGSKSKRVSLLQNPPTVGVATLKLTDVHPSDTGTYLCQVNNPPDFYTNGLGLINLTVLVPPSNPLCSQSGQTSVGGSTALRCSSSEGAPKPVYNWVRLGTFPTPSPGSMVQDEVSGQLILTNLSLTSSGTYRCVATNQMGSASCELTLSVTEPSQGRVAGALIGVLLGVLLLSVAAFCLVRFQKERGKKPKETYGGSDLREDAIAPGISEHTCMRADSSKGFLERPSSASTVTTTKSKLPMVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Sheep
88
Dog
88
Pig
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96IQ7 As Substrate

Site PTM Type Enzyme
S302 Phosphorylation
S303 Phosphorylation
T318 Phosphorylation
T319 Phosphorylation

Research Backgrounds

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in stomach, colon, prostate, trachea and thyroid glands and weakly in bladder and lung.

References

1). Key Candidate Genes–VSIG2 of Colon Cancer Identified by Weighted Gene Co-Expression Network Analysis. Cancer Management and Research, 2021 (PubMed: 34290531) [IF=3.3]

Application: IHC    Species: Human    Sample: cancer tissues

Figure 6 (A) The expression of VSIG2 in normal tissues. (B) The expression of VSIG2 in cancer tissues. (C) The expression of LC3 in cancer tissues.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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