XRN1 Antibody - #DF2433

(2)  
Product: XRN1 Antibody
Catalog: DF2433
Description: Rabbit polyclonal antibody to XRN1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 194 kDa; 194kD(Calculated).
Uniprot: Q8IZH2
RRID: AB_2839639

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
XRN1 Antibody detects endogenous levels of total XRN1.
RRID:
AB_2839639
Cite Format: Affinity Biosciences Cat# DF2433, RRID:AB_2839639.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5'-3' exoribonuclease 1; Dhm2; mXRN1; SEP1; Strand-exchange protein 1 homolog;

Immunogens

Immunogen:
Uniprot:

Q8IZH2(XRN1_HUMAN) >>Visit HPA database.

Gene(ID):
XRN1(54464)
Expression:
Q8IZH2 XRN1_HUMAN:

Expressed in heart, brain, pancreas, spleen, testis, osteogenic sarcoma (OGS) biopsy and primary cell lines.

Sequence:
MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREHFSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLSDIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSPIIEYYPPDFKTDLNGKQQEWEAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFIYPSPWPEKFPAIERCCTRYKIISLDAWRVDINKNKITRIDQKALYFCGFPTLKHIRHKFFLKKSGVQVFQQSSRGENMMLEILVDAESDELTVENVASSVLGKSVFVNWPHLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKVVHLGDKEQSNWAKEVQGISEHYLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFDSRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDALIQNQHKYSIKYNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKKVGSEWMYSSAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLKGHPVSTLSRSSCDLQILDAAIVEKIEEEVEKCKQRKNNKKVRVTVKPHLLYRPLEQQHGVIPDRDAEFCLFDRVVNVRENFSVPVGLRGTIIGIKGANREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVKPQPAVHQHSSSSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVLPQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIENFLASLNISKENEVQSSHHGEPPSEEHLSPQSFAMGTRMLKEILKIDGSNTVDHKNEIKQIANEIPVSSNRRDEYGLPSQPKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVSTPVTELSRICSLVGMPQPDFSFLRMPQTMTVCQVKLSNGLLVHGPQCHSENEAKEKAALFALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPTGWDHYGSNYALGAANIMPSSSHLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKKRVANKKNFENKEAQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHHKSTPISSSRRKSRKLAVNFGVSKPSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
82
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IZH2 As Substrate

Site PTM Type Enzyme
K79 Ubiquitination
K118 Ubiquitination
K268 Acetylation
T270 Phosphorylation
Y273 Phosphorylation
K338 Ubiquitination
K342 Ubiquitination
K364 Ubiquitination
K368 Ubiquitination
Y384 Phosphorylation
K391 Ubiquitination
S396 Phosphorylation
K413 Ubiquitination
K572 Ubiquitination
S584 Phosphorylation
K586 Ubiquitination
K618 Ubiquitination
K652 Ubiquitination
K673 Ubiquitination
K736 Ubiquitination
K747 Ubiquitination
T753 Phosphorylation
K758 Ubiquitination
K765 Ubiquitination
K772 Ubiquitination
Y797 Phosphorylation
T802 Phosphorylation
K805 Ubiquitination
K818 Ubiquitination
Y829 Phosphorylation
S844 Phosphorylation
K847 Ubiquitination
K910 Ubiquitination
R939 Methylation
K950 Ubiquitination
K971 Ubiquitination
Y990 Phosphorylation
K1035 Ubiquitination
K1130 Ubiquitination
S1173 Phosphorylation
S1176 Phosphorylation
K1228 Ubiquitination
K1245 Ubiquitination
Y1248 Phosphorylation
K1256 Ubiquitination
K1272 Ubiquitination
S1279 Phosphorylation
K1281 Ubiquitination
Y1282 Phosphorylation
S1299 Phosphorylation
K1301 Ubiquitination
S1306 Phosphorylation
K1308 Ubiquitination
S1348 Phosphorylation
K1364 Acetylation
K1364 Ubiquitination
K1374 Ubiquitination
K1378 Ubiquitination
Y1394 Phosphorylation
S1398 Phosphorylation
K1401 Ubiquitination
K1412 Acetylation
Y1419 Phosphorylation
S1451 Phosphorylation
K1621 Ubiquitination
T1629 Phosphorylation
T1633 Phosphorylation
S1638 Phosphorylation
S1645 Phosphorylation
S1653 Phosphorylation
S1692 Phosphorylation

Research Backgrounds

Function:

Major 5'-3' exoribonuclease involved in mRNA decay. Required for the 5'-3'-processing of the G4 tetraplex-containing DNA and RNA substrates. The kinetic of hydrolysis is faster for G4 RNA tetraplex than for G4 DNA tetraplex and monomeric RNA tetraplex. Binds to RNA and DNA (By similarity). Plays a role in replication-dependent histone mRNA degradation. May act as a tumor suppressor protein in osteogenic sarcoma (OGS).

Subcellular Location:

Cytoplasm.
Note: Discrete foci at the inner surface of the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, brain, pancreas, spleen, testis, osteogenic sarcoma (OGS) biopsy and primary cell lines.

Subunit Structure:

Found in a mRNP complex with UPF1, UPF2, UPF3B and XRN1. Associates with alpha and beta tubulins (By similarity). Interacts with DIS3L2. Interacts with ZC3HAV1 in an RNA-dependent manner. Interacts with ZFP36L1. Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner. Interacts with YTHDC2 (via ANK repeats).

Family&Domains:

Belongs to the 5'-3' exonuclease family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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