SRRM1 Antibody - #DF2431

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Product: SRRM1 Antibody
Catalog: DF2431
Description: Rabbit polyclonal antibody to SRRM1
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Chicken
Mol.Wt.: 102 kDa; 102kD(Calculated).
Uniprot: Q8IYB3
RRID: AB_2839637

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(86%), Horse(90%), Rabbit(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
SRRM1 Antibody detects endogenous levels of total SRRM1.
RRID:
AB_2839637
Cite Format: Affinity Biosciences Cat# DF2431, RRID:AB_2839637.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ser/Arg related nuclear matrix protein; Ser/Arg-related nuclear matrix protein; Serine/arginine repetitive matrix protein 1; SR related nuclear matrix protein of 160 kDa; SR-related nuclear matrix protein of 160 kDa; SRm160; Srrm1; SRRM1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q8IYB3(SRRM1_HUMAN) >>Visit HPA database.

Gene(ID):
SRRM1(10250)
Description:
Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.
Sequence:
MDAGFFRGTSAEQDNRFSNKQKKLLKQLKFAECLEKKVDMSKVNLEVIKPWITKRVTEILGFEDDVVIEFIFNQLEVKNPDSKMMQINLTGFLNGKNAREFMGELWPLLLSAQENIAGIPSAFLELKKEEIKQRQIEQEKLASMKKQDEDKDKRDKEEKESSREKRERSRSPRRRKSRSPSPRRRSSPVRRERKRSHSRSPRHRTKSRSPSPAPEKKEKTPELPEPSVKVKEPSVQEATSTSDILKVPKPEPIPEPKEPSPEKNSKKEKEKEKTRPRSRSRSKSRSRTRSRSPSHTRPRRRHRSRSRSYSPRRRPSPRRRPSPRRRTPPRRMPPPPRHRRSRSPVRRRRRSSASLSGSSSSSSSSRSRSPPKKPPKRTSSPPRKTRRLSPSASPPRRRHRPSPPATPPPKTRHSPTPQQSNRTRKSRVSVSPGRTSGKVTKHKGTEKRESPSPAPKPRKVELSESEEDKGGKMAAADSVQQRRQYRRQNQQSSSDSGSSSSSEDERPKRSHVKNGEVGRRRRHSPSRSASPSPRKRQKETSPRGRRRRSPSPPPTRRRRSPSPAPPPRRRRTPTPPPRRRTPSPPPRRRSPSPRRYSPPIQRRYSPSPPPKRRTASPPPPPKRRASPSPPPKRRVSHSPPPKQRSSPVTKRRSPSLSSKHRKGSSPSRSTREARSPQPNKRHSPSPRPRAPQTSSSPPPVRRGASSSPQRRQSPSPSTRPIRRVSRTPEPKKIKKAASPSPQSVRRVSSSRSVSGSPEPAAKKPPAPPSPVQSQSPSTNWSPAVPVKKAKSPTPSPSPPRNSDQEGGGKKKKKKKDKKHKKDKKHKKHKKHKKEKAVAAAAAAAVTPAAIAAATTTLAQEEPVAAPEPKKETESEAEDNLDDLEKHLREKALRSMRKAQVSPQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Chicken
91
Horse
90
Bovine
86
Xenopus
70
Sheep
45
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IYB3 As Substrate

Site PTM Type Enzyme
M1 Acetylation
R7 Methylation
T9 Phosphorylation
S10 Phosphorylation
R16 Methylation
S18 Phosphorylation
K29 Acetylation
K29 Ubiquitination
K36 Acetylation
K36 Ubiquitination
K49 Ubiquitination
K54 Ubiquitination
K140 Acetylation
K140 Ubiquitination
S162 Phosphorylation
S177 Phosphorylation
S179 Phosphorylation
S181 Phosphorylation
S186 Phosphorylation
S187 Phosphorylation
S196 Phosphorylation
S198 Phosphorylation
S200 Phosphorylation
T205 Phosphorylation
S207 Phosphorylation
S209 Phosphorylation
S211 Phosphorylation
T220 Phosphorylation
S227 Phosphorylation
K231 Sumoylation
S234 Phosphorylation
T239 Phosphorylation
S240 Phosphorylation
T241 Phosphorylation
S242 Phosphorylation
K249 Sumoylation
S260 Phosphorylation
S265 Phosphorylation
S290 Phosphorylation
S292 Phosphorylation
S294 Phosphorylation
T296 Phosphorylation
S304 Phosphorylation
S306 Phosphorylation
S308 Phosphorylation
Y309 Phosphorylation
S310 Phosphorylation
S316 Phosphorylation
T327 Phosphorylation
S360 Phosphorylation
S367 Phosphorylation
S369 Phosphorylation
S379 Phosphorylation
S380 Phosphorylation
T385 Phosphorylation
S389 Phosphorylation
S391 Phosphorylation
S393 Phosphorylation
S402 Phosphorylation
T406 Phosphorylation
T411 Phosphorylation
S414 Phosphorylation
T416 Phosphorylation
S420 Phosphorylation
T423 Phosphorylation
S426 Phosphorylation
S429 Phosphorylation
S431 Phosphorylation
T435 Phosphorylation
S436 Phosphorylation
K438 Acetylation
T445 Phosphorylation
S450 Phosphorylation
S452 Phosphorylation
S463 Phosphorylation
S465 Phosphorylation
S478 Phosphorylation
S494 Phosphorylation
S500 Phosphorylation
S502 Phosphorylation
S524 Phosphorylation
S526 Phosphorylation
S528 Phosphorylation
S530 Phosphorylation
S532 Phosphorylation
S541 Phosphorylation
S549 Phosphorylation
S551 Phosphorylation
T555 Phosphorylation
S560 Phosphorylation
S562 Phosphorylation
T572 Phosphorylation P42345 (MTOR)
T574 Phosphorylation P42345 (MTOR)
T581 Phosphorylation
S583 Phosphorylation
S590 Phosphorylation
S592 Phosphorylation
Y596 Phosphorylation
S597 Phosphorylation
Y604 Phosphorylation
S605 Phosphorylation
S607 Phosphorylation
T614 Phosphorylation
S616 Phosphorylation
S626 Phosphorylation
S628 Phosphorylation
S636 Phosphorylation
S638 Phosphorylation
S645 Phosphorylation
S646 Phosphorylation
T649 Phosphorylation
S653 Phosphorylation
S655 Phosphorylation
S657 Phosphorylation
S658 Phosphorylation
S665 Phosphorylation
S667 Phosphorylation
S669 Phosphorylation
S675 Phosphorylation
S683 Phosphorylation
S685 Phosphorylation
T693 Phosphorylation
S694 Phosphorylation
S695 Phosphorylation
S696 Phosphorylation
S705 Phosphorylation
S706 Phosphorylation
S707 Phosphorylation
S713 Phosphorylation
S715 Phosphorylation
S717 Phosphorylation
T718 Phosphorylation
S725 Phosphorylation
T727 Phosphorylation
S738 Phosphorylation
S740 Phosphorylation
S743 Phosphorylation
S748 Phosphorylation
S749 Phosphorylation
S750 Phosphorylation
S752 Phosphorylation
S754 Phosphorylation
S756 Phosphorylation
S769 Phosphorylation P24941 (CDK2)
S773 Phosphorylation P24941 (CDK2)
S775 Phosphorylation
S777 Phosphorylation
T778 Phosphorylation
S781 Phosphorylation
S791 Phosphorylation
T793 Phosphorylation
S795 Phosphorylation
S797 Phosphorylation
S802 Phosphorylation
K811 Ubiquitination
K812 Ubiquitination
T846 Phosphorylation
T855 Phosphorylation
T856 Phosphorylation
T872 Phosphorylation
S874 Phosphorylation
S894 Phosphorylation
S901 Phosphorylation
S904 Phosphorylation

Research Backgrounds

Function:

Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates.

PTMs:

Phosphorylated on multiple serine and threonine residues by DYRK3 during the G2-to-M transition, after the nuclear-envelope breakdown. Phosphorylation by DYRK3 promotes disassembly of nuclear speckles.

Citrullinated by PADI4.

Subcellular Location:

Nucleus matrix. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in the spliceosome C complex. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Found in a mRNA splicing-dependent exon junction complex (EJC) with DEK, PRPF8, NCBP1, RBM8A, RNPS1, SRRM1 and ALYREF/THOC4. Interacts with BAT1, CPSF1, RBM8A, RNPS1, and ALYREF/THOC4. Seems to be a compound of RNA export complexes that are released from speckles in a ATP-dependent manner.

Family&Domains:

Belongs to the splicing factor SR family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

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